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Otoprotection Mechanisms Against Oxidative Stress Caused by Cisplatin
Introduction Cisplatin damages the auditory system and is related to the generation of free radicals. Glutathione peroxidase is an endogenous free radicals remover. Objective To investigate the mechanisms involved in otoprotection by N-acetylcysteine through the expression of glutathione peroxidas...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Thieme Revinter Publicações Ltda
2020
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6952293/ https://www.ncbi.nlm.nih.gov/pubmed/31929833 http://dx.doi.org/10.1055/s-0039-1698782 |
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author | Gonçalves, Maiara Santos Silveira, Aron Ferreira da Murashima, Adriana de Andrade Batista Rossato, Maria Hippolito, Miguel Angelo |
author_facet | Gonçalves, Maiara Santos Silveira, Aron Ferreira da Murashima, Adriana de Andrade Batista Rossato, Maria Hippolito, Miguel Angelo |
author_sort | Gonçalves, Maiara Santos |
collection | PubMed |
description | Introduction Cisplatin damages the auditory system and is related to the generation of free radicals. Glutathione peroxidase is an endogenous free radicals remover. Objective To investigate the mechanisms involved in otoprotection by N-acetylcysteine through the expression of glutathione peroxidase in outer hair cells from rats treated with cisplatin. Methods Male Wistar rats were intraperitoneally injected with cisplatin (8 mg/Kg) and/or received oral administration by gavage of N-acetylcysteine (300 mg/Kg) for 3 consecutive days. On the 4 (th) day, the animals were euthanized and beheaded. The tympanic bullae were removed and prepared for scanning electron microscopy and immunofluorescence. Results Among the groups exposed to ototoxic doses of cisplatin, there was an increase in glutathione peroxidase immunostaining in two groups, the one exposed to cisplatin alone, and the group exposed to both cisplatin and N-acetylcysteine. Conclusion The expression of glutathione peroxidase in the outer hair cells of rats exposed to cisplatin showed the synthesis of this enzyme under cellular toxicity conditions. |
format | Online Article Text |
id | pubmed-6952293 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | Thieme Revinter Publicações Ltda |
record_format | MEDLINE/PubMed |
spelling | pubmed-69522932020-01-10 Otoprotection Mechanisms Against Oxidative Stress Caused by Cisplatin Gonçalves, Maiara Santos Silveira, Aron Ferreira da Murashima, Adriana de Andrade Batista Rossato, Maria Hippolito, Miguel Angelo Int Arch Otorhinolaryngol Introduction Cisplatin damages the auditory system and is related to the generation of free radicals. Glutathione peroxidase is an endogenous free radicals remover. Objective To investigate the mechanisms involved in otoprotection by N-acetylcysteine through the expression of glutathione peroxidase in outer hair cells from rats treated with cisplatin. Methods Male Wistar rats were intraperitoneally injected with cisplatin (8 mg/Kg) and/or received oral administration by gavage of N-acetylcysteine (300 mg/Kg) for 3 consecutive days. On the 4 (th) day, the animals were euthanized and beheaded. The tympanic bullae were removed and prepared for scanning electron microscopy and immunofluorescence. Results Among the groups exposed to ototoxic doses of cisplatin, there was an increase in glutathione peroxidase immunostaining in two groups, the one exposed to cisplatin alone, and the group exposed to both cisplatin and N-acetylcysteine. Conclusion The expression of glutathione peroxidase in the outer hair cells of rats exposed to cisplatin showed the synthesis of this enzyme under cellular toxicity conditions. Thieme Revinter Publicações Ltda 2020-01 2020-01-09 /pmc/articles/PMC6952293/ /pubmed/31929833 http://dx.doi.org/10.1055/s-0039-1698782 Text en https://creativecommons.org/licenses/by-nc-nd/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivatives License, which permits unrestricted reproduction and distribution, for non-commercial purposes only; and use and reproduction, but not distribution, of adapted material for non-commercial purposes only, provided the original work is properly cited. |
spellingShingle | Gonçalves, Maiara Santos Silveira, Aron Ferreira da Murashima, Adriana de Andrade Batista Rossato, Maria Hippolito, Miguel Angelo Otoprotection Mechanisms Against Oxidative Stress Caused by Cisplatin |
title | Otoprotection Mechanisms Against Oxidative Stress Caused by Cisplatin |
title_full | Otoprotection Mechanisms Against Oxidative Stress Caused by Cisplatin |
title_fullStr | Otoprotection Mechanisms Against Oxidative Stress Caused by Cisplatin |
title_full_unstemmed | Otoprotection Mechanisms Against Oxidative Stress Caused by Cisplatin |
title_short | Otoprotection Mechanisms Against Oxidative Stress Caused by Cisplatin |
title_sort | otoprotection mechanisms against oxidative stress caused by cisplatin |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6952293/ https://www.ncbi.nlm.nih.gov/pubmed/31929833 http://dx.doi.org/10.1055/s-0039-1698782 |
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