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Hyperglycemia compromises Rat Cortical Bone by Increasing Osteocyte Lacunar Density and Decreasing Vascular Canal Volume

Uncontrolled diabetes is associated with increased risk of bony fractures. However, the mechanisms have yet to be understood. Using high-resolution synchrotron micro-CT, we calculated the changes in the microstructure of femoral cortices of streptozotocin-induced hyperglycemic (STZ) Wistar Albino ra...

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Autores principales: Ay, Birol, Parolia, Kushagra, Liddell, Robert S., Qiu, Yusheng, Grasselli, Giovanni, Cooper, David M. L., Davies, John E.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6952406/
https://www.ncbi.nlm.nih.gov/pubmed/31925331
http://dx.doi.org/10.1038/s42003-019-0747-1
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author Ay, Birol
Parolia, Kushagra
Liddell, Robert S.
Qiu, Yusheng
Grasselli, Giovanni
Cooper, David M. L.
Davies, John E.
author_facet Ay, Birol
Parolia, Kushagra
Liddell, Robert S.
Qiu, Yusheng
Grasselli, Giovanni
Cooper, David M. L.
Davies, John E.
author_sort Ay, Birol
collection PubMed
description Uncontrolled diabetes is associated with increased risk of bony fractures. However, the mechanisms have yet to be understood. Using high-resolution synchrotron micro-CT, we calculated the changes in the microstructure of femoral cortices of streptozotocin-induced hyperglycemic (STZ) Wistar Albino rats and tested the mechanical properties of the mineralized matrix by nanoindentation. Total lacunar volume of femoral cortices increased in STZ group due to a 9% increase in lacunar density. However, total vascular canal volume decreased in STZ group due to a remarkable decrease in vascular canal diameter (7 ± 0.3 vs. 8.5 ± 0.4 µm). Osteocytic territorial matrix volume was less in the STZ group (14,908 ± 689 µm(3)) compared with healthy controls (16,367 ± 391 µm(3)). In conclusion, hyperglycemia increased cellularity and lacunar density, decreased osteocyte territorial matrix, and reduced vascular girth, in addition to decreasing matrix mechanical properties in the STZ group when compared with euglycemic controls.
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spelling pubmed-69524062020-01-13 Hyperglycemia compromises Rat Cortical Bone by Increasing Osteocyte Lacunar Density and Decreasing Vascular Canal Volume Ay, Birol Parolia, Kushagra Liddell, Robert S. Qiu, Yusheng Grasselli, Giovanni Cooper, David M. L. Davies, John E. Commun Biol Article Uncontrolled diabetes is associated with increased risk of bony fractures. However, the mechanisms have yet to be understood. Using high-resolution synchrotron micro-CT, we calculated the changes in the microstructure of femoral cortices of streptozotocin-induced hyperglycemic (STZ) Wistar Albino rats and tested the mechanical properties of the mineralized matrix by nanoindentation. Total lacunar volume of femoral cortices increased in STZ group due to a 9% increase in lacunar density. However, total vascular canal volume decreased in STZ group due to a remarkable decrease in vascular canal diameter (7 ± 0.3 vs. 8.5 ± 0.4 µm). Osteocytic territorial matrix volume was less in the STZ group (14,908 ± 689 µm(3)) compared with healthy controls (16,367 ± 391 µm(3)). In conclusion, hyperglycemia increased cellularity and lacunar density, decreased osteocyte territorial matrix, and reduced vascular girth, in addition to decreasing matrix mechanical properties in the STZ group when compared with euglycemic controls. Nature Publishing Group UK 2020-01-09 /pmc/articles/PMC6952406/ /pubmed/31925331 http://dx.doi.org/10.1038/s42003-019-0747-1 Text en © The Author(s) 2020 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
Ay, Birol
Parolia, Kushagra
Liddell, Robert S.
Qiu, Yusheng
Grasselli, Giovanni
Cooper, David M. L.
Davies, John E.
Hyperglycemia compromises Rat Cortical Bone by Increasing Osteocyte Lacunar Density and Decreasing Vascular Canal Volume
title Hyperglycemia compromises Rat Cortical Bone by Increasing Osteocyte Lacunar Density and Decreasing Vascular Canal Volume
title_full Hyperglycemia compromises Rat Cortical Bone by Increasing Osteocyte Lacunar Density and Decreasing Vascular Canal Volume
title_fullStr Hyperglycemia compromises Rat Cortical Bone by Increasing Osteocyte Lacunar Density and Decreasing Vascular Canal Volume
title_full_unstemmed Hyperglycemia compromises Rat Cortical Bone by Increasing Osteocyte Lacunar Density and Decreasing Vascular Canal Volume
title_short Hyperglycemia compromises Rat Cortical Bone by Increasing Osteocyte Lacunar Density and Decreasing Vascular Canal Volume
title_sort hyperglycemia compromises rat cortical bone by increasing osteocyte lacunar density and decreasing vascular canal volume
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6952406/
https://www.ncbi.nlm.nih.gov/pubmed/31925331
http://dx.doi.org/10.1038/s42003-019-0747-1
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