Establishment of sperm cryopreservation and in vitro fertilisation protocols for rats

Recently, genome-editing tools have come into common use in the field of rat research, and consequently, many genetically modified rat strains have been preserved and archived as frozen embryos. Although there have been many reports published on the topic of rat sperm cryopreservation, no report has...

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Autores principales: Nakagata, Naomi, Mikoda, Nobuyuki, Nakao, Satohiro, Nakatsukasa, Ena, Takeo, Toru
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2020
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6952447/
https://www.ncbi.nlm.nih.gov/pubmed/31919412
http://dx.doi.org/10.1038/s41598-019-57090-7
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author Nakagata, Naomi
Mikoda, Nobuyuki
Nakao, Satohiro
Nakatsukasa, Ena
Takeo, Toru
author_facet Nakagata, Naomi
Mikoda, Nobuyuki
Nakao, Satohiro
Nakatsukasa, Ena
Takeo, Toru
author_sort Nakagata, Naomi
collection PubMed
description Recently, genome-editing tools have come into common use in the field of rat research, and consequently, many genetically modified rat strains have been preserved and archived as frozen embryos. Although there have been many reports published on the topic of rat sperm cryopreservation, no report has yet provided satisfactory and acceptable protocols for the cryopreservation of rat sperm. In this study, we developed methods for both the cryopreservation of transgenic rat sperm and in vitro fertilisation using frozen sperm, which yielded high fertilisation rates.
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spelling pubmed-69524472020-01-14 Establishment of sperm cryopreservation and in vitro fertilisation protocols for rats Nakagata, Naomi Mikoda, Nobuyuki Nakao, Satohiro Nakatsukasa, Ena Takeo, Toru Sci Rep Article Recently, genome-editing tools have come into common use in the field of rat research, and consequently, many genetically modified rat strains have been preserved and archived as frozen embryos. Although there have been many reports published on the topic of rat sperm cryopreservation, no report has yet provided satisfactory and acceptable protocols for the cryopreservation of rat sperm. In this study, we developed methods for both the cryopreservation of transgenic rat sperm and in vitro fertilisation using frozen sperm, which yielded high fertilisation rates. Nature Publishing Group UK 2020-01-09 /pmc/articles/PMC6952447/ /pubmed/31919412 http://dx.doi.org/10.1038/s41598-019-57090-7 Text en © The Author(s) 2020 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
Nakagata, Naomi
Mikoda, Nobuyuki
Nakao, Satohiro
Nakatsukasa, Ena
Takeo, Toru
Establishment of sperm cryopreservation and in vitro fertilisation protocols for rats
title Establishment of sperm cryopreservation and in vitro fertilisation protocols for rats
title_full Establishment of sperm cryopreservation and in vitro fertilisation protocols for rats
title_fullStr Establishment of sperm cryopreservation and in vitro fertilisation protocols for rats
title_full_unstemmed Establishment of sperm cryopreservation and in vitro fertilisation protocols for rats
title_short Establishment of sperm cryopreservation and in vitro fertilisation protocols for rats
title_sort establishment of sperm cryopreservation and in vitro fertilisation protocols for rats
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6952447/
https://www.ncbi.nlm.nih.gov/pubmed/31919412
http://dx.doi.org/10.1038/s41598-019-57090-7
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