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Synovial-Fluid miRNA Signature for Diagnosis of Juvenile Idiopathic Arthritis

Juvenile idiopathic arthritis (JIA) is the most common chronic inflammatory rheumatism in childhood; microRNAs (miRNAs) have been proposed as diagnostic biomarkers. Although joints are the primary targets for JIA, a synovial fluid-based miRNA signature has never been studied. We aim to identify miRN...

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Autores principales: Nziza, Nadége, Jeziorski, Eric, Delpont, Marion, Cren, Maïlys, Chevassus, Hugues, Carbasse, Aurélia, Mahe, Perrine, Abassi, Hamouda, Joly-Monrigal, Pauline, Schordan, Eric, Mangé, Alain, Jorgensen, Christian, Apparailly, Florence, Duroux-Richard, Isabelle
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6952798/
https://www.ncbi.nlm.nih.gov/pubmed/31779271
http://dx.doi.org/10.3390/cells8121521
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author Nziza, Nadége
Jeziorski, Eric
Delpont, Marion
Cren, Maïlys
Chevassus, Hugues
Carbasse, Aurélia
Mahe, Perrine
Abassi, Hamouda
Joly-Monrigal, Pauline
Schordan, Eric
Mangé, Alain
Jorgensen, Christian
Apparailly, Florence
Duroux-Richard, Isabelle
author_facet Nziza, Nadége
Jeziorski, Eric
Delpont, Marion
Cren, Maïlys
Chevassus, Hugues
Carbasse, Aurélia
Mahe, Perrine
Abassi, Hamouda
Joly-Monrigal, Pauline
Schordan, Eric
Mangé, Alain
Jorgensen, Christian
Apparailly, Florence
Duroux-Richard, Isabelle
author_sort Nziza, Nadége
collection PubMed
description Juvenile idiopathic arthritis (JIA) is the most common chronic inflammatory rheumatism in childhood; microRNAs (miRNAs) have been proposed as diagnostic biomarkers. Although joints are the primary targets for JIA, a synovial fluid-based miRNA signature has never been studied. We aim to identify miRNA biomarkers in JIA by comparing synovial fluid and serum samples from children with JIA and K. kingae septic arthritis (SA). With next-generation high-throughput sequencing, we measured the absolute levels of 2083 miRNAs in synovial fluid and serum from an exploratory cohort of children and validated differentially expressed miRNAs in a replication study by using RT-qPCR. We identified a 19-miRNA signature only in synovial fluid samples that was significantly deregulated, with at least 2-fold change in expression, in JIA versus SA (p < 0.01). The combination of miR-6764-5p, miR-155, and miR-146a-5p expression in synovial fluid yielded an area under the receiver operating characteristic curve of 1 (95% CI 0.978 to 1), thereby perfectly differentiating JIA from SA in children. We propose, for the first time, a synovial fluid-specific miRNA signature for JIA and associated signaling pathways that may indicate potential biomarkers to assist in the classification and differential diagnosis of JIA and help in understanding JIA pathogenesis.
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spelling pubmed-69527982020-01-23 Synovial-Fluid miRNA Signature for Diagnosis of Juvenile Idiopathic Arthritis Nziza, Nadége Jeziorski, Eric Delpont, Marion Cren, Maïlys Chevassus, Hugues Carbasse, Aurélia Mahe, Perrine Abassi, Hamouda Joly-Monrigal, Pauline Schordan, Eric Mangé, Alain Jorgensen, Christian Apparailly, Florence Duroux-Richard, Isabelle Cells Article Juvenile idiopathic arthritis (JIA) is the most common chronic inflammatory rheumatism in childhood; microRNAs (miRNAs) have been proposed as diagnostic biomarkers. Although joints are the primary targets for JIA, a synovial fluid-based miRNA signature has never been studied. We aim to identify miRNA biomarkers in JIA by comparing synovial fluid and serum samples from children with JIA and K. kingae septic arthritis (SA). With next-generation high-throughput sequencing, we measured the absolute levels of 2083 miRNAs in synovial fluid and serum from an exploratory cohort of children and validated differentially expressed miRNAs in a replication study by using RT-qPCR. We identified a 19-miRNA signature only in synovial fluid samples that was significantly deregulated, with at least 2-fold change in expression, in JIA versus SA (p < 0.01). The combination of miR-6764-5p, miR-155, and miR-146a-5p expression in synovial fluid yielded an area under the receiver operating characteristic curve of 1 (95% CI 0.978 to 1), thereby perfectly differentiating JIA from SA in children. We propose, for the first time, a synovial fluid-specific miRNA signature for JIA and associated signaling pathways that may indicate potential biomarkers to assist in the classification and differential diagnosis of JIA and help in understanding JIA pathogenesis. MDPI 2019-11-26 /pmc/articles/PMC6952798/ /pubmed/31779271 http://dx.doi.org/10.3390/cells8121521 Text en © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Nziza, Nadége
Jeziorski, Eric
Delpont, Marion
Cren, Maïlys
Chevassus, Hugues
Carbasse, Aurélia
Mahe, Perrine
Abassi, Hamouda
Joly-Monrigal, Pauline
Schordan, Eric
Mangé, Alain
Jorgensen, Christian
Apparailly, Florence
Duroux-Richard, Isabelle
Synovial-Fluid miRNA Signature for Diagnosis of Juvenile Idiopathic Arthritis
title Synovial-Fluid miRNA Signature for Diagnosis of Juvenile Idiopathic Arthritis
title_full Synovial-Fluid miRNA Signature for Diagnosis of Juvenile Idiopathic Arthritis
title_fullStr Synovial-Fluid miRNA Signature for Diagnosis of Juvenile Idiopathic Arthritis
title_full_unstemmed Synovial-Fluid miRNA Signature for Diagnosis of Juvenile Idiopathic Arthritis
title_short Synovial-Fluid miRNA Signature for Diagnosis of Juvenile Idiopathic Arthritis
title_sort synovial-fluid mirna signature for diagnosis of juvenile idiopathic arthritis
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6952798/
https://www.ncbi.nlm.nih.gov/pubmed/31779271
http://dx.doi.org/10.3390/cells8121521
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