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Sheathless Shape-Based Separation of Candida Albicans Using a Viscoelastic Non-Newtonian Fluid

Rapid and accurate identification of Candida albicans from among other candida species is critical for cost-effective treatment and antifungal drug assays. Shape is a critical biomarker indicating cell type, cell cycle, and environmental conditions; however, most microfluidic techniques have been fo...

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Autores principales: Nam, Jeonghun, Jee, Hyunseul, Jang, Woong Sik, Yoon, Jung, Park, Borae G., Lee, Seong Jae, Lim, Chae Seung
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6952941/
https://www.ncbi.nlm.nih.gov/pubmed/31779188
http://dx.doi.org/10.3390/mi10120817
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author Nam, Jeonghun
Jee, Hyunseul
Jang, Woong Sik
Yoon, Jung
Park, Borae G.
Lee, Seong Jae
Lim, Chae Seung
author_facet Nam, Jeonghun
Jee, Hyunseul
Jang, Woong Sik
Yoon, Jung
Park, Borae G.
Lee, Seong Jae
Lim, Chae Seung
author_sort Nam, Jeonghun
collection PubMed
description Rapid and accurate identification of Candida albicans from among other candida species is critical for cost-effective treatment and antifungal drug assays. Shape is a critical biomarker indicating cell type, cell cycle, and environmental conditions; however, most microfluidic techniques have been focused only on size-based particle/cell manipulation. This study demonstrates a sheathless shape-based separation of particles/cells using a viscoelastic non-Newtonian fluid. The size of C. albicans was measured at 37 °C depending on the incubation time (0 h, 1 h, and 2 h). The effects of flow rates on the flow patterns of candida cells with different shapes were examined. Finally, 2-h-incubated candida cells with germ tube formations (≥26 μm) were separated from spherical candida cells and shorter candida cells with a separation efficiency of 80.9% and a purity of 91.2% at 50 μL/min.
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spelling pubmed-69529412020-01-23 Sheathless Shape-Based Separation of Candida Albicans Using a Viscoelastic Non-Newtonian Fluid Nam, Jeonghun Jee, Hyunseul Jang, Woong Sik Yoon, Jung Park, Borae G. Lee, Seong Jae Lim, Chae Seung Micromachines (Basel) Article Rapid and accurate identification of Candida albicans from among other candida species is critical for cost-effective treatment and antifungal drug assays. Shape is a critical biomarker indicating cell type, cell cycle, and environmental conditions; however, most microfluidic techniques have been focused only on size-based particle/cell manipulation. This study demonstrates a sheathless shape-based separation of particles/cells using a viscoelastic non-Newtonian fluid. The size of C. albicans was measured at 37 °C depending on the incubation time (0 h, 1 h, and 2 h). The effects of flow rates on the flow patterns of candida cells with different shapes were examined. Finally, 2-h-incubated candida cells with germ tube formations (≥26 μm) were separated from spherical candida cells and shorter candida cells with a separation efficiency of 80.9% and a purity of 91.2% at 50 μL/min. MDPI 2019-11-26 /pmc/articles/PMC6952941/ /pubmed/31779188 http://dx.doi.org/10.3390/mi10120817 Text en © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Nam, Jeonghun
Jee, Hyunseul
Jang, Woong Sik
Yoon, Jung
Park, Borae G.
Lee, Seong Jae
Lim, Chae Seung
Sheathless Shape-Based Separation of Candida Albicans Using a Viscoelastic Non-Newtonian Fluid
title Sheathless Shape-Based Separation of Candida Albicans Using a Viscoelastic Non-Newtonian Fluid
title_full Sheathless Shape-Based Separation of Candida Albicans Using a Viscoelastic Non-Newtonian Fluid
title_fullStr Sheathless Shape-Based Separation of Candida Albicans Using a Viscoelastic Non-Newtonian Fluid
title_full_unstemmed Sheathless Shape-Based Separation of Candida Albicans Using a Viscoelastic Non-Newtonian Fluid
title_short Sheathless Shape-Based Separation of Candida Albicans Using a Viscoelastic Non-Newtonian Fluid
title_sort sheathless shape-based separation of candida albicans using a viscoelastic non-newtonian fluid
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6952941/
https://www.ncbi.nlm.nih.gov/pubmed/31779188
http://dx.doi.org/10.3390/mi10120817
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