Cargando…

Impedance-Based Biosensing of Pseudomonas putida via Solution Blow Spun PLA: MWCNT Composite Nanofibers

Quantifiable sensing of common microbes in chronic wounds has the potential to enable an objective assessment of wound healing for diagnostic applications. Sensing platforms should be robust, simple, and flexible to provide clinicians with a point-of-care tool. In this work, solution blow spun poly...

Descripción completa

Detalles Bibliográficos
Autores principales: Miller, Craig, Stiglich, Madison, Livingstone, Mark, Gilmore, Jordon
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6952954/
https://www.ncbi.nlm.nih.gov/pubmed/31847091
http://dx.doi.org/10.3390/mi10120876
_version_ 1783486539285659648
author Miller, Craig
Stiglich, Madison
Livingstone, Mark
Gilmore, Jordon
author_facet Miller, Craig
Stiglich, Madison
Livingstone, Mark
Gilmore, Jordon
author_sort Miller, Craig
collection PubMed
description Quantifiable sensing of common microbes in chronic wounds has the potential to enable an objective assessment of wound healing for diagnostic applications. Sensing platforms should be robust, simple, and flexible to provide clinicians with a point-of-care tool. In this work, solution blow spun poly (lactic acid)/multiwalled carbon nanotube nanofiber composites are used to detect the presence and concentration of Pseudomonas putida in vitro using changes in impedance. Impedance microbiology (IM) is a well-documented diagnostic technique used in many applications, including cancer detection, tuberculosis screening and pregnancy tests. Twenty-four hour real-time measurements of the equivalent circuit of three culture media were taken with an inductance, capacitance, and resistance (LCR) meter. Variations in impedance were calculated to correspond to the growth of P. putida. Additionally, instantaneous measurements of bacterial cultures were taken over a one-minute time point to display the fast sensing of bacterial load via IM. This proof-of-concept shows that conductive solution blow spun fiber mats is a valid fabrication technique to develop in situ wound dressing impedance sensors. Study results indicate successful measurement and quantification of bacterial growth in this proof-of-concept study.
format Online
Article
Text
id pubmed-6952954
institution National Center for Biotechnology Information
language English
publishDate 2019
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-69529542020-01-23 Impedance-Based Biosensing of Pseudomonas putida via Solution Blow Spun PLA: MWCNT Composite Nanofibers Miller, Craig Stiglich, Madison Livingstone, Mark Gilmore, Jordon Micromachines (Basel) Article Quantifiable sensing of common microbes in chronic wounds has the potential to enable an objective assessment of wound healing for diagnostic applications. Sensing platforms should be robust, simple, and flexible to provide clinicians with a point-of-care tool. In this work, solution blow spun poly (lactic acid)/multiwalled carbon nanotube nanofiber composites are used to detect the presence and concentration of Pseudomonas putida in vitro using changes in impedance. Impedance microbiology (IM) is a well-documented diagnostic technique used in many applications, including cancer detection, tuberculosis screening and pregnancy tests. Twenty-four hour real-time measurements of the equivalent circuit of three culture media were taken with an inductance, capacitance, and resistance (LCR) meter. Variations in impedance were calculated to correspond to the growth of P. putida. Additionally, instantaneous measurements of bacterial cultures were taken over a one-minute time point to display the fast sensing of bacterial load via IM. This proof-of-concept shows that conductive solution blow spun fiber mats is a valid fabrication technique to develop in situ wound dressing impedance sensors. Study results indicate successful measurement and quantification of bacterial growth in this proof-of-concept study. MDPI 2019-12-13 /pmc/articles/PMC6952954/ /pubmed/31847091 http://dx.doi.org/10.3390/mi10120876 Text en © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Miller, Craig
Stiglich, Madison
Livingstone, Mark
Gilmore, Jordon
Impedance-Based Biosensing of Pseudomonas putida via Solution Blow Spun PLA: MWCNT Composite Nanofibers
title Impedance-Based Biosensing of Pseudomonas putida via Solution Blow Spun PLA: MWCNT Composite Nanofibers
title_full Impedance-Based Biosensing of Pseudomonas putida via Solution Blow Spun PLA: MWCNT Composite Nanofibers
title_fullStr Impedance-Based Biosensing of Pseudomonas putida via Solution Blow Spun PLA: MWCNT Composite Nanofibers
title_full_unstemmed Impedance-Based Biosensing of Pseudomonas putida via Solution Blow Spun PLA: MWCNT Composite Nanofibers
title_short Impedance-Based Biosensing of Pseudomonas putida via Solution Blow Spun PLA: MWCNT Composite Nanofibers
title_sort impedance-based biosensing of pseudomonas putida via solution blow spun pla: mwcnt composite nanofibers
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6952954/
https://www.ncbi.nlm.nih.gov/pubmed/31847091
http://dx.doi.org/10.3390/mi10120876
work_keys_str_mv AT millercraig impedancebasedbiosensingofpseudomonasputidaviasolutionblowspunplamwcntcompositenanofibers
AT stiglichmadison impedancebasedbiosensingofpseudomonasputidaviasolutionblowspunplamwcntcompositenanofibers
AT livingstonemark impedancebasedbiosensingofpseudomonasputidaviasolutionblowspunplamwcntcompositenanofibers
AT gilmorejordon impedancebasedbiosensingofpseudomonasputidaviasolutionblowspunplamwcntcompositenanofibers