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Impedance-Based Biosensing of Pseudomonas putida via Solution Blow Spun PLA: MWCNT Composite Nanofibers
Quantifiable sensing of common microbes in chronic wounds has the potential to enable an objective assessment of wound healing for diagnostic applications. Sensing platforms should be robust, simple, and flexible to provide clinicians with a point-of-care tool. In this work, solution blow spun poly...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6952954/ https://www.ncbi.nlm.nih.gov/pubmed/31847091 http://dx.doi.org/10.3390/mi10120876 |
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author | Miller, Craig Stiglich, Madison Livingstone, Mark Gilmore, Jordon |
author_facet | Miller, Craig Stiglich, Madison Livingstone, Mark Gilmore, Jordon |
author_sort | Miller, Craig |
collection | PubMed |
description | Quantifiable sensing of common microbes in chronic wounds has the potential to enable an objective assessment of wound healing for diagnostic applications. Sensing platforms should be robust, simple, and flexible to provide clinicians with a point-of-care tool. In this work, solution blow spun poly (lactic acid)/multiwalled carbon nanotube nanofiber composites are used to detect the presence and concentration of Pseudomonas putida in vitro using changes in impedance. Impedance microbiology (IM) is a well-documented diagnostic technique used in many applications, including cancer detection, tuberculosis screening and pregnancy tests. Twenty-four hour real-time measurements of the equivalent circuit of three culture media were taken with an inductance, capacitance, and resistance (LCR) meter. Variations in impedance were calculated to correspond to the growth of P. putida. Additionally, instantaneous measurements of bacterial cultures were taken over a one-minute time point to display the fast sensing of bacterial load via IM. This proof-of-concept shows that conductive solution blow spun fiber mats is a valid fabrication technique to develop in situ wound dressing impedance sensors. Study results indicate successful measurement and quantification of bacterial growth in this proof-of-concept study. |
format | Online Article Text |
id | pubmed-6952954 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-69529542020-01-23 Impedance-Based Biosensing of Pseudomonas putida via Solution Blow Spun PLA: MWCNT Composite Nanofibers Miller, Craig Stiglich, Madison Livingstone, Mark Gilmore, Jordon Micromachines (Basel) Article Quantifiable sensing of common microbes in chronic wounds has the potential to enable an objective assessment of wound healing for diagnostic applications. Sensing platforms should be robust, simple, and flexible to provide clinicians with a point-of-care tool. In this work, solution blow spun poly (lactic acid)/multiwalled carbon nanotube nanofiber composites are used to detect the presence and concentration of Pseudomonas putida in vitro using changes in impedance. Impedance microbiology (IM) is a well-documented diagnostic technique used in many applications, including cancer detection, tuberculosis screening and pregnancy tests. Twenty-four hour real-time measurements of the equivalent circuit of three culture media were taken with an inductance, capacitance, and resistance (LCR) meter. Variations in impedance were calculated to correspond to the growth of P. putida. Additionally, instantaneous measurements of bacterial cultures were taken over a one-minute time point to display the fast sensing of bacterial load via IM. This proof-of-concept shows that conductive solution blow spun fiber mats is a valid fabrication technique to develop in situ wound dressing impedance sensors. Study results indicate successful measurement and quantification of bacterial growth in this proof-of-concept study. MDPI 2019-12-13 /pmc/articles/PMC6952954/ /pubmed/31847091 http://dx.doi.org/10.3390/mi10120876 Text en © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Miller, Craig Stiglich, Madison Livingstone, Mark Gilmore, Jordon Impedance-Based Biosensing of Pseudomonas putida via Solution Blow Spun PLA: MWCNT Composite Nanofibers |
title | Impedance-Based Biosensing of Pseudomonas putida via Solution Blow Spun PLA: MWCNT Composite Nanofibers |
title_full | Impedance-Based Biosensing of Pseudomonas putida via Solution Blow Spun PLA: MWCNT Composite Nanofibers |
title_fullStr | Impedance-Based Biosensing of Pseudomonas putida via Solution Blow Spun PLA: MWCNT Composite Nanofibers |
title_full_unstemmed | Impedance-Based Biosensing of Pseudomonas putida via Solution Blow Spun PLA: MWCNT Composite Nanofibers |
title_short | Impedance-Based Biosensing of Pseudomonas putida via Solution Blow Spun PLA: MWCNT Composite Nanofibers |
title_sort | impedance-based biosensing of pseudomonas putida via solution blow spun pla: mwcnt composite nanofibers |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6952954/ https://www.ncbi.nlm.nih.gov/pubmed/31847091 http://dx.doi.org/10.3390/mi10120876 |
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