Cargando…

Sex Differences in Liver, Adipose Tissue, and Muscle Transcriptional Response to Fasting and Refeeding in Mice

Fasting is often used for obesity correction but the “refeeding syndrome” limits its efficiency, and molecular mechanisms underlying metabolic response to different food availability are under investigation. Sex was shown to affect hormonal and metabolic reactions to fasting/refeeding. The aim of th...

Descripción completa

Detalles Bibliográficos
Autores principales: Bazhan, Nadezhda, Jakovleva, Tatiana, Feofanova, Natalia, Denisova, Elena, Dubinina, Anastasia, Sitnikova, Natalia, Makarova, Elena
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6953068/
https://www.ncbi.nlm.nih.gov/pubmed/31783664
http://dx.doi.org/10.3390/cells8121529
_version_ 1783486566217285632
author Bazhan, Nadezhda
Jakovleva, Tatiana
Feofanova, Natalia
Denisova, Elena
Dubinina, Anastasia
Sitnikova, Natalia
Makarova, Elena
author_facet Bazhan, Nadezhda
Jakovleva, Tatiana
Feofanova, Natalia
Denisova, Elena
Dubinina, Anastasia
Sitnikova, Natalia
Makarova, Elena
author_sort Bazhan, Nadezhda
collection PubMed
description Fasting is often used for obesity correction but the “refeeding syndrome” limits its efficiency, and molecular mechanisms underlying metabolic response to different food availability are under investigation. Sex was shown to affect hormonal and metabolic reactions to fasting/refeeding. The aim of this study was to evaluate hormonal and transcriptional responses to fasting and refeeding in male and female C57Bl/6J mice. Sex asymmetry was observed both at the hormonal and transcriptional levels. Fasting (24 h) induced increase in hepatic Fgf21 gene expression, which was associated with elevation of plasma FGF21 and adiponectin levels, and the upregulation of expression of hepatic (Pparα, Cpt1α) and muscle (Cpt1β, Ucp3) genes involved in fatty acid oxidation. These changes were more pronounced in females. Refeeding (6 h) evoked hyperinsulinemia and increased hepatic expression of gene related to lipogenesis (Fasn) only in males and hyperleptinemia and increase in Fgf21 gene expression in muscles and adipose tissues only in females. The results suggest that in mice, one of the molecular mechanisms underlying sex asymmetry in hepatic Pparα, Cpt1α, muscle Cpt1β, and Ucp3 expression during fasting is hepatic Fgf21 expression, and the reason for sex asymmetry in hepatic Fasn expression during refeeding is male-specific hyperinsulinemia.
format Online
Article
Text
id pubmed-6953068
institution National Center for Biotechnology Information
language English
publishDate 2019
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-69530682020-01-23 Sex Differences in Liver, Adipose Tissue, and Muscle Transcriptional Response to Fasting and Refeeding in Mice Bazhan, Nadezhda Jakovleva, Tatiana Feofanova, Natalia Denisova, Elena Dubinina, Anastasia Sitnikova, Natalia Makarova, Elena Cells Article Fasting is often used for obesity correction but the “refeeding syndrome” limits its efficiency, and molecular mechanisms underlying metabolic response to different food availability are under investigation. Sex was shown to affect hormonal and metabolic reactions to fasting/refeeding. The aim of this study was to evaluate hormonal and transcriptional responses to fasting and refeeding in male and female C57Bl/6J mice. Sex asymmetry was observed both at the hormonal and transcriptional levels. Fasting (24 h) induced increase in hepatic Fgf21 gene expression, which was associated with elevation of plasma FGF21 and adiponectin levels, and the upregulation of expression of hepatic (Pparα, Cpt1α) and muscle (Cpt1β, Ucp3) genes involved in fatty acid oxidation. These changes were more pronounced in females. Refeeding (6 h) evoked hyperinsulinemia and increased hepatic expression of gene related to lipogenesis (Fasn) only in males and hyperleptinemia and increase in Fgf21 gene expression in muscles and adipose tissues only in females. The results suggest that in mice, one of the molecular mechanisms underlying sex asymmetry in hepatic Pparα, Cpt1α, muscle Cpt1β, and Ucp3 expression during fasting is hepatic Fgf21 expression, and the reason for sex asymmetry in hepatic Fasn expression during refeeding is male-specific hyperinsulinemia. MDPI 2019-11-27 /pmc/articles/PMC6953068/ /pubmed/31783664 http://dx.doi.org/10.3390/cells8121529 Text en © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Bazhan, Nadezhda
Jakovleva, Tatiana
Feofanova, Natalia
Denisova, Elena
Dubinina, Anastasia
Sitnikova, Natalia
Makarova, Elena
Sex Differences in Liver, Adipose Tissue, and Muscle Transcriptional Response to Fasting and Refeeding in Mice
title Sex Differences in Liver, Adipose Tissue, and Muscle Transcriptional Response to Fasting and Refeeding in Mice
title_full Sex Differences in Liver, Adipose Tissue, and Muscle Transcriptional Response to Fasting and Refeeding in Mice
title_fullStr Sex Differences in Liver, Adipose Tissue, and Muscle Transcriptional Response to Fasting and Refeeding in Mice
title_full_unstemmed Sex Differences in Liver, Adipose Tissue, and Muscle Transcriptional Response to Fasting and Refeeding in Mice
title_short Sex Differences in Liver, Adipose Tissue, and Muscle Transcriptional Response to Fasting and Refeeding in Mice
title_sort sex differences in liver, adipose tissue, and muscle transcriptional response to fasting and refeeding in mice
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6953068/
https://www.ncbi.nlm.nih.gov/pubmed/31783664
http://dx.doi.org/10.3390/cells8121529
work_keys_str_mv AT bazhannadezhda sexdifferencesinliveradiposetissueandmuscletranscriptionalresponsetofastingandrefeedinginmice
AT jakovlevatatiana sexdifferencesinliveradiposetissueandmuscletranscriptionalresponsetofastingandrefeedinginmice
AT feofanovanatalia sexdifferencesinliveradiposetissueandmuscletranscriptionalresponsetofastingandrefeedinginmice
AT denisovaelena sexdifferencesinliveradiposetissueandmuscletranscriptionalresponsetofastingandrefeedinginmice
AT dubininaanastasia sexdifferencesinliveradiposetissueandmuscletranscriptionalresponsetofastingandrefeedinginmice
AT sitnikovanatalia sexdifferencesinliveradiposetissueandmuscletranscriptionalresponsetofastingandrefeedinginmice
AT makarovaelena sexdifferencesinliveradiposetissueandmuscletranscriptionalresponsetofastingandrefeedinginmice