Cargando…

Critical Impact of Human Amniotic Membrane Tension on Mitochondrial Function and Cell Viability In Vitro

Amniotic cells show exciting stem cell features, which has led to the idea of using living cells of human amniotic membranes (hAMs) in toto for clinical applications. However, under common cell culture conditions, viability of amniotic cells decreases rapidly, whereby reasons for this decrease are u...

Descripción completa

Detalles Bibliográficos
Autores principales: Poženel, Laura, Lindenmair, Andrea, Schmidt, Katy, Kozlov, Andrey V., Grillari, Johannes, Wolbank, Susanne, Banerjee, Asmita, Weidinger, Adelheid
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6953074/
https://www.ncbi.nlm.nih.gov/pubmed/31847452
http://dx.doi.org/10.3390/cells8121641
_version_ 1783486567616086016
author Poženel, Laura
Lindenmair, Andrea
Schmidt, Katy
Kozlov, Andrey V.
Grillari, Johannes
Wolbank, Susanne
Banerjee, Asmita
Weidinger, Adelheid
author_facet Poženel, Laura
Lindenmair, Andrea
Schmidt, Katy
Kozlov, Andrey V.
Grillari, Johannes
Wolbank, Susanne
Banerjee, Asmita
Weidinger, Adelheid
author_sort Poženel, Laura
collection PubMed
description Amniotic cells show exciting stem cell features, which has led to the idea of using living cells of human amniotic membranes (hAMs) in toto for clinical applications. However, under common cell culture conditions, viability of amniotic cells decreases rapidly, whereby reasons for this decrease are unknown so far. Recently, it has been suggested that loss of tissue tension in vivo leads to apoptosis. Therefore, the aim of this study was to investigate the effect of tissue distention on the viability of amniotic cells in vitro. Thereby, particular focus was put on vital mitochondria-linked parameters, such as respiration and ATP synthesis. Biopsies of hAMs were incubated for 7–21 days either non-distended or distended. We observed increased B-cell lymphoma 2-associated X protein (BAX)/B-cell lymphoma (BCL)-2 ratios in non-distended hAMs at day seven, followed by increased caspase 3 expression at day 14, and, consequently, loss of viability at day 21. In contrast, under distention, caspase 3 expression increased only slightly, and mitochondrial function and cellular viability were largely maintained. Our data suggest that a mechano-sensing pathway may control viability of hAM cells by triggering mitochondria-mediated apoptosis upon loss of tension in vitro. Further studies are required to elucidate the underlying molecular mechanisms between tissue distention and viability of hAM cells.
format Online
Article
Text
id pubmed-6953074
institution National Center for Biotechnology Information
language English
publishDate 2019
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-69530742020-01-23 Critical Impact of Human Amniotic Membrane Tension on Mitochondrial Function and Cell Viability In Vitro Poženel, Laura Lindenmair, Andrea Schmidt, Katy Kozlov, Andrey V. Grillari, Johannes Wolbank, Susanne Banerjee, Asmita Weidinger, Adelheid Cells Communication Amniotic cells show exciting stem cell features, which has led to the idea of using living cells of human amniotic membranes (hAMs) in toto for clinical applications. However, under common cell culture conditions, viability of amniotic cells decreases rapidly, whereby reasons for this decrease are unknown so far. Recently, it has been suggested that loss of tissue tension in vivo leads to apoptosis. Therefore, the aim of this study was to investigate the effect of tissue distention on the viability of amniotic cells in vitro. Thereby, particular focus was put on vital mitochondria-linked parameters, such as respiration and ATP synthesis. Biopsies of hAMs were incubated for 7–21 days either non-distended or distended. We observed increased B-cell lymphoma 2-associated X protein (BAX)/B-cell lymphoma (BCL)-2 ratios in non-distended hAMs at day seven, followed by increased caspase 3 expression at day 14, and, consequently, loss of viability at day 21. In contrast, under distention, caspase 3 expression increased only slightly, and mitochondrial function and cellular viability were largely maintained. Our data suggest that a mechano-sensing pathway may control viability of hAM cells by triggering mitochondria-mediated apoptosis upon loss of tension in vitro. Further studies are required to elucidate the underlying molecular mechanisms between tissue distention and viability of hAM cells. MDPI 2019-12-15 /pmc/articles/PMC6953074/ /pubmed/31847452 http://dx.doi.org/10.3390/cells8121641 Text en © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Communication
Poženel, Laura
Lindenmair, Andrea
Schmidt, Katy
Kozlov, Andrey V.
Grillari, Johannes
Wolbank, Susanne
Banerjee, Asmita
Weidinger, Adelheid
Critical Impact of Human Amniotic Membrane Tension on Mitochondrial Function and Cell Viability In Vitro
title Critical Impact of Human Amniotic Membrane Tension on Mitochondrial Function and Cell Viability In Vitro
title_full Critical Impact of Human Amniotic Membrane Tension on Mitochondrial Function and Cell Viability In Vitro
title_fullStr Critical Impact of Human Amniotic Membrane Tension on Mitochondrial Function and Cell Viability In Vitro
title_full_unstemmed Critical Impact of Human Amniotic Membrane Tension on Mitochondrial Function and Cell Viability In Vitro
title_short Critical Impact of Human Amniotic Membrane Tension on Mitochondrial Function and Cell Viability In Vitro
title_sort critical impact of human amniotic membrane tension on mitochondrial function and cell viability in vitro
topic Communication
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6953074/
https://www.ncbi.nlm.nih.gov/pubmed/31847452
http://dx.doi.org/10.3390/cells8121641
work_keys_str_mv AT pozenellaura criticalimpactofhumanamnioticmembranetensiononmitochondrialfunctionandcellviabilityinvitro
AT lindenmairandrea criticalimpactofhumanamnioticmembranetensiononmitochondrialfunctionandcellviabilityinvitro
AT schmidtkaty criticalimpactofhumanamnioticmembranetensiononmitochondrialfunctionandcellviabilityinvitro
AT kozlovandreyv criticalimpactofhumanamnioticmembranetensiononmitochondrialfunctionandcellviabilityinvitro
AT grillarijohannes criticalimpactofhumanamnioticmembranetensiononmitochondrialfunctionandcellviabilityinvitro
AT wolbanksusanne criticalimpactofhumanamnioticmembranetensiononmitochondrialfunctionandcellviabilityinvitro
AT banerjeeasmita criticalimpactofhumanamnioticmembranetensiononmitochondrialfunctionandcellviabilityinvitro
AT weidingeradelheid criticalimpactofhumanamnioticmembranetensiononmitochondrialfunctionandcellviabilityinvitro