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A Fully Integrated In Vitro Diagnostic Microsystem for Pathogen Detection Developed Using a “3D Extensible” Microfluidic Design Paradigm
Microfluidics is facing critical challenges in the quest of miniaturizing, integrating, and automating in vitro diagnostics, including the increasing complexity of assays, the gap between the macroscale world and the microscale devices, and the diverse throughput demands in various clinical settings...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6953088/ https://www.ncbi.nlm.nih.gov/pubmed/31842384 http://dx.doi.org/10.3390/mi10120873 |
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author | Geng, Zhi Gu, Yin Li, Shanglin Lin, Baobao Liu, Peng |
author_facet | Geng, Zhi Gu, Yin Li, Shanglin Lin, Baobao Liu, Peng |
author_sort | Geng, Zhi |
collection | PubMed |
description | Microfluidics is facing critical challenges in the quest of miniaturizing, integrating, and automating in vitro diagnostics, including the increasing complexity of assays, the gap between the macroscale world and the microscale devices, and the diverse throughput demands in various clinical settings. Here, a “3D extensible” microfluidic design paradigm that consists of a set of basic structures and unit operations was developed for constructing any application-specific assay. Four basic structures—check valve (in), check valve (out), double-check valve (in and out), and on–off valve—were designed to mimic basic acts in biochemical assays. By combining these structures linearly, a series of unit operations can be readily formed. We then proposed a “3D extensible” architecture to fulfill the needs of the function integration, the adaptive “world-to-chip” interface, and the adjustable throughput in the X, Y, and Z directions, respectively. To verify this design paradigm, we developed a fully integrated loop-mediated isothermal amplification microsystem that can directly accept swab samples and detect Chlamydia trachomatis automatically with a sensitivity one order higher than that of the conventional kit. This demonstration validated the feasibility of using this paradigm to develop integrated and automated microsystems in a less risky and more consistent manner. |
format | Online Article Text |
id | pubmed-6953088 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-69530882020-01-23 A Fully Integrated In Vitro Diagnostic Microsystem for Pathogen Detection Developed Using a “3D Extensible” Microfluidic Design Paradigm Geng, Zhi Gu, Yin Li, Shanglin Lin, Baobao Liu, Peng Micromachines (Basel) Article Microfluidics is facing critical challenges in the quest of miniaturizing, integrating, and automating in vitro diagnostics, including the increasing complexity of assays, the gap between the macroscale world and the microscale devices, and the diverse throughput demands in various clinical settings. Here, a “3D extensible” microfluidic design paradigm that consists of a set of basic structures and unit operations was developed for constructing any application-specific assay. Four basic structures—check valve (in), check valve (out), double-check valve (in and out), and on–off valve—were designed to mimic basic acts in biochemical assays. By combining these structures linearly, a series of unit operations can be readily formed. We then proposed a “3D extensible” architecture to fulfill the needs of the function integration, the adaptive “world-to-chip” interface, and the adjustable throughput in the X, Y, and Z directions, respectively. To verify this design paradigm, we developed a fully integrated loop-mediated isothermal amplification microsystem that can directly accept swab samples and detect Chlamydia trachomatis automatically with a sensitivity one order higher than that of the conventional kit. This demonstration validated the feasibility of using this paradigm to develop integrated and automated microsystems in a less risky and more consistent manner. MDPI 2019-12-12 /pmc/articles/PMC6953088/ /pubmed/31842384 http://dx.doi.org/10.3390/mi10120873 Text en © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Geng, Zhi Gu, Yin Li, Shanglin Lin, Baobao Liu, Peng A Fully Integrated In Vitro Diagnostic Microsystem for Pathogen Detection Developed Using a “3D Extensible” Microfluidic Design Paradigm |
title | A Fully Integrated In Vitro Diagnostic Microsystem for Pathogen Detection Developed Using a “3D Extensible” Microfluidic Design Paradigm |
title_full | A Fully Integrated In Vitro Diagnostic Microsystem for Pathogen Detection Developed Using a “3D Extensible” Microfluidic Design Paradigm |
title_fullStr | A Fully Integrated In Vitro Diagnostic Microsystem for Pathogen Detection Developed Using a “3D Extensible” Microfluidic Design Paradigm |
title_full_unstemmed | A Fully Integrated In Vitro Diagnostic Microsystem for Pathogen Detection Developed Using a “3D Extensible” Microfluidic Design Paradigm |
title_short | A Fully Integrated In Vitro Diagnostic Microsystem for Pathogen Detection Developed Using a “3D Extensible” Microfluidic Design Paradigm |
title_sort | fully integrated in vitro diagnostic microsystem for pathogen detection developed using a “3d extensible” microfluidic design paradigm |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6953088/ https://www.ncbi.nlm.nih.gov/pubmed/31842384 http://dx.doi.org/10.3390/mi10120873 |
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