Cargando…

A Foldable Chip Array for the Continuous Investigation of Seed Germination and the Subsequent Root Development of Seedlings

Seed germination and seedling root development are important indicators of plant development. This work designed and fabricated a foldable microfluidic chip array for conducting nondestructive and continuous evaluation of seed germination and subsequent seedling development in situ. Each plant chamb...

Descripción completa

Detalles Bibliográficos
Autores principales: Song, Zhao Xi, Chai, Hui Hui, Chen, Feng, Yu, Ling, Fang, Can
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6953092/
https://www.ncbi.nlm.nih.gov/pubmed/31861063
http://dx.doi.org/10.3390/mi10120884
_version_ 1783486571889033216
author Song, Zhao Xi
Chai, Hui Hui
Chen, Feng
Yu, Ling
Fang, Can
author_facet Song, Zhao Xi
Chai, Hui Hui
Chen, Feng
Yu, Ling
Fang, Can
author_sort Song, Zhao Xi
collection PubMed
description Seed germination and seedling root development are important indicators of plant development. This work designed and fabricated a foldable microfluidic chip array for conducting nondestructive and continuous evaluation of seed germination and subsequent seedling development in situ. Each plant chamber has two functional units: seed germination part and root-growth part. The root-growth parts are themselves connected to a single channel designed to provide a uniform culture medium for plant growth. The individual chips are connected into an array using elastic hinges that facilitate the folding and unfolding of the array to accommodate different viewing purposes. In the folded state, the seed germination chambers form a closely spaced array platform to facilitate the comparison of seed germination and plant development characteristics. Unfolding the array facilitates a clear examination of root development within the root-growth parts. The observation window of an individual chip facilitates either the direct examination of the developing seedling (e.g., stems and leaves) or the use of a microscope for examining microscale features (e.g., root tips and root hairs). The potential of the proposed foldable chip array as a new cultivation platform for botanic studies is demonstrated by examining the seed germination and seedling development of tobacco (Nicotiana tabacum) under different cultivation conditions.
format Online
Article
Text
id pubmed-6953092
institution National Center for Biotechnology Information
language English
publishDate 2019
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-69530922020-01-23 A Foldable Chip Array for the Continuous Investigation of Seed Germination and the Subsequent Root Development of Seedlings Song, Zhao Xi Chai, Hui Hui Chen, Feng Yu, Ling Fang, Can Micromachines (Basel) Article Seed germination and seedling root development are important indicators of plant development. This work designed and fabricated a foldable microfluidic chip array for conducting nondestructive and continuous evaluation of seed germination and subsequent seedling development in situ. Each plant chamber has two functional units: seed germination part and root-growth part. The root-growth parts are themselves connected to a single channel designed to provide a uniform culture medium for plant growth. The individual chips are connected into an array using elastic hinges that facilitate the folding and unfolding of the array to accommodate different viewing purposes. In the folded state, the seed germination chambers form a closely spaced array platform to facilitate the comparison of seed germination and plant development characteristics. Unfolding the array facilitates a clear examination of root development within the root-growth parts. The observation window of an individual chip facilitates either the direct examination of the developing seedling (e.g., stems and leaves) or the use of a microscope for examining microscale features (e.g., root tips and root hairs). The potential of the proposed foldable chip array as a new cultivation platform for botanic studies is demonstrated by examining the seed germination and seedling development of tobacco (Nicotiana tabacum) under different cultivation conditions. MDPI 2019-12-17 /pmc/articles/PMC6953092/ /pubmed/31861063 http://dx.doi.org/10.3390/mi10120884 Text en © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Song, Zhao Xi
Chai, Hui Hui
Chen, Feng
Yu, Ling
Fang, Can
A Foldable Chip Array for the Continuous Investigation of Seed Germination and the Subsequent Root Development of Seedlings
title A Foldable Chip Array for the Continuous Investigation of Seed Germination and the Subsequent Root Development of Seedlings
title_full A Foldable Chip Array for the Continuous Investigation of Seed Germination and the Subsequent Root Development of Seedlings
title_fullStr A Foldable Chip Array for the Continuous Investigation of Seed Germination and the Subsequent Root Development of Seedlings
title_full_unstemmed A Foldable Chip Array for the Continuous Investigation of Seed Germination and the Subsequent Root Development of Seedlings
title_short A Foldable Chip Array for the Continuous Investigation of Seed Germination and the Subsequent Root Development of Seedlings
title_sort foldable chip array for the continuous investigation of seed germination and the subsequent root development of seedlings
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6953092/
https://www.ncbi.nlm.nih.gov/pubmed/31861063
http://dx.doi.org/10.3390/mi10120884
work_keys_str_mv AT songzhaoxi afoldablechiparrayforthecontinuousinvestigationofseedgerminationandthesubsequentrootdevelopmentofseedlings
AT chaihuihui afoldablechiparrayforthecontinuousinvestigationofseedgerminationandthesubsequentrootdevelopmentofseedlings
AT chenfeng afoldablechiparrayforthecontinuousinvestigationofseedgerminationandthesubsequentrootdevelopmentofseedlings
AT yuling afoldablechiparrayforthecontinuousinvestigationofseedgerminationandthesubsequentrootdevelopmentofseedlings
AT fangcan afoldablechiparrayforthecontinuousinvestigationofseedgerminationandthesubsequentrootdevelopmentofseedlings
AT songzhaoxi foldablechiparrayforthecontinuousinvestigationofseedgerminationandthesubsequentrootdevelopmentofseedlings
AT chaihuihui foldablechiparrayforthecontinuousinvestigationofseedgerminationandthesubsequentrootdevelopmentofseedlings
AT chenfeng foldablechiparrayforthecontinuousinvestigationofseedgerminationandthesubsequentrootdevelopmentofseedlings
AT yuling foldablechiparrayforthecontinuousinvestigationofseedgerminationandthesubsequentrootdevelopmentofseedlings
AT fangcan foldablechiparrayforthecontinuousinvestigationofseedgerminationandthesubsequentrootdevelopmentofseedlings