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A Foldable Chip Array for the Continuous Investigation of Seed Germination and the Subsequent Root Development of Seedlings
Seed germination and seedling root development are important indicators of plant development. This work designed and fabricated a foldable microfluidic chip array for conducting nondestructive and continuous evaluation of seed germination and subsequent seedling development in situ. Each plant chamb...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6953092/ https://www.ncbi.nlm.nih.gov/pubmed/31861063 http://dx.doi.org/10.3390/mi10120884 |
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author | Song, Zhao Xi Chai, Hui Hui Chen, Feng Yu, Ling Fang, Can |
author_facet | Song, Zhao Xi Chai, Hui Hui Chen, Feng Yu, Ling Fang, Can |
author_sort | Song, Zhao Xi |
collection | PubMed |
description | Seed germination and seedling root development are important indicators of plant development. This work designed and fabricated a foldable microfluidic chip array for conducting nondestructive and continuous evaluation of seed germination and subsequent seedling development in situ. Each plant chamber has two functional units: seed germination part and root-growth part. The root-growth parts are themselves connected to a single channel designed to provide a uniform culture medium for plant growth. The individual chips are connected into an array using elastic hinges that facilitate the folding and unfolding of the array to accommodate different viewing purposes. In the folded state, the seed germination chambers form a closely spaced array platform to facilitate the comparison of seed germination and plant development characteristics. Unfolding the array facilitates a clear examination of root development within the root-growth parts. The observation window of an individual chip facilitates either the direct examination of the developing seedling (e.g., stems and leaves) or the use of a microscope for examining microscale features (e.g., root tips and root hairs). The potential of the proposed foldable chip array as a new cultivation platform for botanic studies is demonstrated by examining the seed germination and seedling development of tobacco (Nicotiana tabacum) under different cultivation conditions. |
format | Online Article Text |
id | pubmed-6953092 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-69530922020-01-23 A Foldable Chip Array for the Continuous Investigation of Seed Germination and the Subsequent Root Development of Seedlings Song, Zhao Xi Chai, Hui Hui Chen, Feng Yu, Ling Fang, Can Micromachines (Basel) Article Seed germination and seedling root development are important indicators of plant development. This work designed and fabricated a foldable microfluidic chip array for conducting nondestructive and continuous evaluation of seed germination and subsequent seedling development in situ. Each plant chamber has two functional units: seed germination part and root-growth part. The root-growth parts are themselves connected to a single channel designed to provide a uniform culture medium for plant growth. The individual chips are connected into an array using elastic hinges that facilitate the folding and unfolding of the array to accommodate different viewing purposes. In the folded state, the seed germination chambers form a closely spaced array platform to facilitate the comparison of seed germination and plant development characteristics. Unfolding the array facilitates a clear examination of root development within the root-growth parts. The observation window of an individual chip facilitates either the direct examination of the developing seedling (e.g., stems and leaves) or the use of a microscope for examining microscale features (e.g., root tips and root hairs). The potential of the proposed foldable chip array as a new cultivation platform for botanic studies is demonstrated by examining the seed germination and seedling development of tobacco (Nicotiana tabacum) under different cultivation conditions. MDPI 2019-12-17 /pmc/articles/PMC6953092/ /pubmed/31861063 http://dx.doi.org/10.3390/mi10120884 Text en © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Song, Zhao Xi Chai, Hui Hui Chen, Feng Yu, Ling Fang, Can A Foldable Chip Array for the Continuous Investigation of Seed Germination and the Subsequent Root Development of Seedlings |
title | A Foldable Chip Array for the Continuous Investigation of Seed Germination and the Subsequent Root Development of Seedlings |
title_full | A Foldable Chip Array for the Continuous Investigation of Seed Germination and the Subsequent Root Development of Seedlings |
title_fullStr | A Foldable Chip Array for the Continuous Investigation of Seed Germination and the Subsequent Root Development of Seedlings |
title_full_unstemmed | A Foldable Chip Array for the Continuous Investigation of Seed Germination and the Subsequent Root Development of Seedlings |
title_short | A Foldable Chip Array for the Continuous Investigation of Seed Germination and the Subsequent Root Development of Seedlings |
title_sort | foldable chip array for the continuous investigation of seed germination and the subsequent root development of seedlings |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6953092/ https://www.ncbi.nlm.nih.gov/pubmed/31861063 http://dx.doi.org/10.3390/mi10120884 |
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