Different effects of cortisol on pro-inflammatory gene expressions in LPS-, heat-killed E.coli-, or live E.coli-stimulated bovine endometrial epithelial cells

BACKGROUND: Bacterial infections are common in postpartum dairy cows. Cortisol level has been observed to increase in dairy cows during peripartum period, and is associated with the endometrial innate immunity against pathogens like E.coli. However, the mechanism underlying how cortisol regulates E....

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Autores principales: Cui, Luying, Wang, Yali, Wang, Heng, Dong, Junsheng, Li, Zixiang, Li, Jun, Qian, Chen, Li, Jianji
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2020
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6953302/
https://www.ncbi.nlm.nih.gov/pubmed/31918707
http://dx.doi.org/10.1186/s12917-020-2231-z
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author Cui, Luying
Wang, Yali
Wang, Heng
Dong, Junsheng
Li, Zixiang
Li, Jun
Qian, Chen
Li, Jianji
author_facet Cui, Luying
Wang, Yali
Wang, Heng
Dong, Junsheng
Li, Zixiang
Li, Jun
Qian, Chen
Li, Jianji
author_sort Cui, Luying
collection PubMed
description BACKGROUND: Bacterial infections are common in postpartum dairy cows. Cortisol level has been observed to increase in dairy cows during peripartum period, and is associated with the endometrial innate immunity against pathogens like E.coli. However, the mechanism underlying how cortisol regulates E.coli-induced inflammatory response in bovine endometrial epithelial cells (BEEC) remains elusive. RESULTS: Cortisol decreased the expressions of IL1β, IL6, TNF-α, IL8, and TLR4 mRNA in BEEC treated with LPS or heat-killed E.coli, but up-regulated these gene expressions in BEEC stimulated by live E.coli. CONCLUSION: Cortisol exerted the anti-inflammatory action on LPS- or heat-killed E.coli-stimulated BEEC, but the pro-inflammatory action on live E.coli-induced BEEC.
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spelling pubmed-69533022020-01-14 Different effects of cortisol on pro-inflammatory gene expressions in LPS-, heat-killed E.coli-, or live E.coli-stimulated bovine endometrial epithelial cells Cui, Luying Wang, Yali Wang, Heng Dong, Junsheng Li, Zixiang Li, Jun Qian, Chen Li, Jianji BMC Vet Res Research Article BACKGROUND: Bacterial infections are common in postpartum dairy cows. Cortisol level has been observed to increase in dairy cows during peripartum period, and is associated with the endometrial innate immunity against pathogens like E.coli. However, the mechanism underlying how cortisol regulates E.coli-induced inflammatory response in bovine endometrial epithelial cells (BEEC) remains elusive. RESULTS: Cortisol decreased the expressions of IL1β, IL6, TNF-α, IL8, and TLR4 mRNA in BEEC treated with LPS or heat-killed E.coli, but up-regulated these gene expressions in BEEC stimulated by live E.coli. CONCLUSION: Cortisol exerted the anti-inflammatory action on LPS- or heat-killed E.coli-stimulated BEEC, but the pro-inflammatory action on live E.coli-induced BEEC. BioMed Central 2020-01-09 /pmc/articles/PMC6953302/ /pubmed/31918707 http://dx.doi.org/10.1186/s12917-020-2231-z Text en © The Author(s). 2020 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
spellingShingle Research Article
Cui, Luying
Wang, Yali
Wang, Heng
Dong, Junsheng
Li, Zixiang
Li, Jun
Qian, Chen
Li, Jianji
Different effects of cortisol on pro-inflammatory gene expressions in LPS-, heat-killed E.coli-, or live E.coli-stimulated bovine endometrial epithelial cells
title Different effects of cortisol on pro-inflammatory gene expressions in LPS-, heat-killed E.coli-, or live E.coli-stimulated bovine endometrial epithelial cells
title_full Different effects of cortisol on pro-inflammatory gene expressions in LPS-, heat-killed E.coli-, or live E.coli-stimulated bovine endometrial epithelial cells
title_fullStr Different effects of cortisol on pro-inflammatory gene expressions in LPS-, heat-killed E.coli-, or live E.coli-stimulated bovine endometrial epithelial cells
title_full_unstemmed Different effects of cortisol on pro-inflammatory gene expressions in LPS-, heat-killed E.coli-, or live E.coli-stimulated bovine endometrial epithelial cells
title_short Different effects of cortisol on pro-inflammatory gene expressions in LPS-, heat-killed E.coli-, or live E.coli-stimulated bovine endometrial epithelial cells
title_sort different effects of cortisol on pro-inflammatory gene expressions in lps-, heat-killed e.coli-, or live e.coli-stimulated bovine endometrial epithelial cells
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6953302/
https://www.ncbi.nlm.nih.gov/pubmed/31918707
http://dx.doi.org/10.1186/s12917-020-2231-z
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