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Immobilized Enzymes on Graphene as Nanobiocatalyst

[Image: see text] Using enzymes as bioelectrocatalysts is an important step toward the next level of biotechnology for energy production. In such biocatalysts, a sacrificial cofactor as an electron and proton source is needed. This is a great obstacle for upscaling, due to cofactor instability and p...

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Autores principales: Seelajaroen, Hathaichanok, Bakandritsos, Aristides, Otyepka, Michal, Zbořil, Radek, Sariciftci, Niyazi Serdar
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Chemical Society 2019
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6953471/
https://www.ncbi.nlm.nih.gov/pubmed/31816230
http://dx.doi.org/10.1021/acsami.9b17777
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author Seelajaroen, Hathaichanok
Bakandritsos, Aristides
Otyepka, Michal
Zbořil, Radek
Sariciftci, Niyazi Serdar
author_facet Seelajaroen, Hathaichanok
Bakandritsos, Aristides
Otyepka, Michal
Zbořil, Radek
Sariciftci, Niyazi Serdar
author_sort Seelajaroen, Hathaichanok
collection PubMed
description [Image: see text] Using enzymes as bioelectrocatalysts is an important step toward the next level of biotechnology for energy production. In such biocatalysts, a sacrificial cofactor as an electron and proton source is needed. This is a great obstacle for upscaling, due to cofactor instability and product separation issues, which increase the costs. Here, we report a cofactor-free electroreduction of CO(2) to a high energy density chemical (methanol) catalyzed by enzyme–graphene hybrids. The biocatalyst consists of dehydrogenases covalently bound on a well-defined carboxyl graphene derivative, serving the role of a conductive nanoplatform. This nanobiocatalyst achieves reduction of CO(2) to methanol at high current densities, which remain unchanged for at least 20 h of operation, without production of other soluble byproducts. It is thus shown that critical improvements on the stability and rate of methanol production at a high Faradaic efficiency of 12% are possible, due to the effective electrochemical process from the electrode to the enzymes via the graphene platform.
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spelling pubmed-69534712020-01-13 Immobilized Enzymes on Graphene as Nanobiocatalyst Seelajaroen, Hathaichanok Bakandritsos, Aristides Otyepka, Michal Zbořil, Radek Sariciftci, Niyazi Serdar ACS Appl Mater Interfaces [Image: see text] Using enzymes as bioelectrocatalysts is an important step toward the next level of biotechnology for energy production. In such biocatalysts, a sacrificial cofactor as an electron and proton source is needed. This is a great obstacle for upscaling, due to cofactor instability and product separation issues, which increase the costs. Here, we report a cofactor-free electroreduction of CO(2) to a high energy density chemical (methanol) catalyzed by enzyme–graphene hybrids. The biocatalyst consists of dehydrogenases covalently bound on a well-defined carboxyl graphene derivative, serving the role of a conductive nanoplatform. This nanobiocatalyst achieves reduction of CO(2) to methanol at high current densities, which remain unchanged for at least 20 h of operation, without production of other soluble byproducts. It is thus shown that critical improvements on the stability and rate of methanol production at a high Faradaic efficiency of 12% are possible, due to the effective electrochemical process from the electrode to the enzymes via the graphene platform. American Chemical Society 2019-12-09 2020-01-08 /pmc/articles/PMC6953471/ /pubmed/31816230 http://dx.doi.org/10.1021/acsami.9b17777 Text en Copyright © 2019 American Chemical Society This is an open access article published under a Creative Commons Attribution (CC-BY) License (http://pubs.acs.org/page/policy/authorchoice_ccby_termsofuse.html) , which permits unrestricted use, distribution and reproduction in any medium, provided the author and source are cited.
spellingShingle Seelajaroen, Hathaichanok
Bakandritsos, Aristides
Otyepka, Michal
Zbořil, Radek
Sariciftci, Niyazi Serdar
Immobilized Enzymes on Graphene as Nanobiocatalyst
title Immobilized Enzymes on Graphene as Nanobiocatalyst
title_full Immobilized Enzymes on Graphene as Nanobiocatalyst
title_fullStr Immobilized Enzymes on Graphene as Nanobiocatalyst
title_full_unstemmed Immobilized Enzymes on Graphene as Nanobiocatalyst
title_short Immobilized Enzymes on Graphene as Nanobiocatalyst
title_sort immobilized enzymes on graphene as nanobiocatalyst
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6953471/
https://www.ncbi.nlm.nih.gov/pubmed/31816230
http://dx.doi.org/10.1021/acsami.9b17777
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