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Inflammasome expression is higher in ovarian tumors than in normal ovary
Chronic inflammation fundamentally influences cancer risk and development. A mechanism of chronic inflammation is the formation of inflammasome complexes which results in the sustained secretion of the pro-inflammatory cytokines IL1β and IL18. Inflammasome expression and actions vary among cancers....
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6953783/ https://www.ncbi.nlm.nih.gov/pubmed/31923221 http://dx.doi.org/10.1371/journal.pone.0227081 |
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author | Luborsky, Judith Barua, Animesh Edassery, Seara Bahr, Janice M. Edassery, Seby L. |
author_facet | Luborsky, Judith Barua, Animesh Edassery, Seara Bahr, Janice M. Edassery, Seby L. |
author_sort | Luborsky, Judith |
collection | PubMed |
description | Chronic inflammation fundamentally influences cancer risk and development. A mechanism of chronic inflammation is the formation of inflammasome complexes which results in the sustained secretion of the pro-inflammatory cytokines IL1β and IL18. Inflammasome expression and actions vary among cancers. There is no information on inflammasome expression in ovarian cancer (OvCa). To determine if ovarian tumors express inflammasome components, mRNA and protein expression of NLRP3 (nucleotide-binding domain, leucine-rich repeat family, pyrin domain containing 3), caspase-1, IL1β, and IL18 expression in hen and human OvCa was assessed. Chicken (hen) OvCa a valid model of spontaneous human OvCa. Hens were selected into study groups with or without tumors using ultrasonography; tumors were confirmed by histology, increased cellular proliferation, and expression of immune cell marker mRNA. mRNA expression was higher for hallmarks of inflammasome activity (caspase-1, 5.9x increase, p = 0.04; IL1β, 4x increase, p = 0.04; and IL18, 7.8x increase, p = 0.0003) in hen OvCa compared to normal ovary. NLRP3, caspase-8 and caspase-11 mRNA did not differ significantly between tumor and non-tumor containing ovaries. Similar results occurred for human OvCa. Protein expression by immunohistochemistry paralleled mRNA expression and was qualitatively higher in tumors. Increased protein expression of caspase-1, IL1β, and IL18 occurred in surface epithelium, tumor cells, and immune cells. The aryl hydrocarbon receptor (AHR), a potential tumor suppressor and NLRP3 regulator, was higher in hen (2.4x increase, p = 0.002) and human tumors (1.8x increase, p = 0.038), suggesting a role in OvCa. Collectively, the results indicate that inflammasome expression is associated with hen and human OvCa, although the NLR sensor type remains to be determined. |
format | Online Article Text |
id | pubmed-6953783 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-69537832020-01-21 Inflammasome expression is higher in ovarian tumors than in normal ovary Luborsky, Judith Barua, Animesh Edassery, Seara Bahr, Janice M. Edassery, Seby L. PLoS One Research Article Chronic inflammation fundamentally influences cancer risk and development. A mechanism of chronic inflammation is the formation of inflammasome complexes which results in the sustained secretion of the pro-inflammatory cytokines IL1β and IL18. Inflammasome expression and actions vary among cancers. There is no information on inflammasome expression in ovarian cancer (OvCa). To determine if ovarian tumors express inflammasome components, mRNA and protein expression of NLRP3 (nucleotide-binding domain, leucine-rich repeat family, pyrin domain containing 3), caspase-1, IL1β, and IL18 expression in hen and human OvCa was assessed. Chicken (hen) OvCa a valid model of spontaneous human OvCa. Hens were selected into study groups with or without tumors using ultrasonography; tumors were confirmed by histology, increased cellular proliferation, and expression of immune cell marker mRNA. mRNA expression was higher for hallmarks of inflammasome activity (caspase-1, 5.9x increase, p = 0.04; IL1β, 4x increase, p = 0.04; and IL18, 7.8x increase, p = 0.0003) in hen OvCa compared to normal ovary. NLRP3, caspase-8 and caspase-11 mRNA did not differ significantly between tumor and non-tumor containing ovaries. Similar results occurred for human OvCa. Protein expression by immunohistochemistry paralleled mRNA expression and was qualitatively higher in tumors. Increased protein expression of caspase-1, IL1β, and IL18 occurred in surface epithelium, tumor cells, and immune cells. The aryl hydrocarbon receptor (AHR), a potential tumor suppressor and NLRP3 regulator, was higher in hen (2.4x increase, p = 0.002) and human tumors (1.8x increase, p = 0.038), suggesting a role in OvCa. Collectively, the results indicate that inflammasome expression is associated with hen and human OvCa, although the NLR sensor type remains to be determined. Public Library of Science 2020-01-10 /pmc/articles/PMC6953783/ /pubmed/31923221 http://dx.doi.org/10.1371/journal.pone.0227081 Text en © 2020 Luborsky et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
spellingShingle | Research Article Luborsky, Judith Barua, Animesh Edassery, Seara Bahr, Janice M. Edassery, Seby L. Inflammasome expression is higher in ovarian tumors than in normal ovary |
title | Inflammasome expression is higher in ovarian tumors than in normal ovary |
title_full | Inflammasome expression is higher in ovarian tumors than in normal ovary |
title_fullStr | Inflammasome expression is higher in ovarian tumors than in normal ovary |
title_full_unstemmed | Inflammasome expression is higher in ovarian tumors than in normal ovary |
title_short | Inflammasome expression is higher in ovarian tumors than in normal ovary |
title_sort | inflammasome expression is higher in ovarian tumors than in normal ovary |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6953783/ https://www.ncbi.nlm.nih.gov/pubmed/31923221 http://dx.doi.org/10.1371/journal.pone.0227081 |
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