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Knockdown of CD-74 in the Proliferative and Apoptotic Activity of Breast Cancer Cells
BACKGROUND: The cluster of differentiation (CD) 74 is known for its immunological functions and its elevated level was reported in various cancer cells. AIM: The aim of the present study was to investigate the expression and potential roles of CD74 in the proliferative and apoptotic activity of brea...
Autores principales: | , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Republic of Macedonia
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6953917/ https://www.ncbi.nlm.nih.gov/pubmed/31949511 http://dx.doi.org/10.3889/oamjms.2019.354 |
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author | Ssadh, Hussain Al Abdulmonem, Waleed Al Rasheed, Zafar Madar, Inamul Hasan Alhoderi, Jamila Eldeen, Samah K. Nasr Alradhwan, Ali Alasmael, Noura Alkhamiss, Abdullah Fernández, Nelson |
author_facet | Ssadh, Hussain Al Abdulmonem, Waleed Al Rasheed, Zafar Madar, Inamul Hasan Alhoderi, Jamila Eldeen, Samah K. Nasr Alradhwan, Ali Alasmael, Noura Alkhamiss, Abdullah Fernández, Nelson |
author_sort | Ssadh, Hussain Al |
collection | PubMed |
description | BACKGROUND: The cluster of differentiation (CD) 74 is known for its immunological functions and its elevated level was reported in various cancer cells. AIM: The aim of the present study was to investigate the expression and potential roles of CD74 in the proliferative and apoptotic activity of breast cancer. METHODS: Expression of CD74, macrophage migration inhibitory factor (MIF) and CD44 was assayed in CAMA-1 and MDA-MB-231 cell lines using flow cytometry. CD74 was knocked down using CD74 siRNA-transfection in CAMA-1, and MDA-MB-231 cells and proliferation and apoptosis were determined in the transfected breast cancer cells. RESULTS: The data showed that CD74, MIF and CD44 were expressed in breast cancer cell lines and were associated with cell proliferation and apoptosis. Correlation analysis revealed that CD74 was positively correlated and colocalised with MIF on the cell-surface of CAMA-1 and MDA-MB-231. The knockdown of CD74 significantly reduced CAMA-1 and MDA-MB-231 cell proliferation and increased the level of apoptotic cells. CONCLUSION: We concluded that the interactions of CD74 with MIF and CD74 with CD44 could be a potential tumour marker for breast cancer cells. Moreover, the level of co-expression of MIF and CD74 or CD44 could be a surrogate marker for the efficacy of anti-angiogenic drugs, particularly in breast cancer tumours. In short, the study revealed the potential roles of CD74 in the proliferation and apoptosis of breast cancer which may serve as a potential therapeutic target for breast cancer. |
format | Online Article Text |
id | pubmed-6953917 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | Republic of Macedonia |
record_format | MEDLINE/PubMed |
spelling | pubmed-69539172020-01-16 Knockdown of CD-74 in the Proliferative and Apoptotic Activity of Breast Cancer Cells Ssadh, Hussain Al Abdulmonem, Waleed Al Rasheed, Zafar Madar, Inamul Hasan Alhoderi, Jamila Eldeen, Samah K. Nasr Alradhwan, Ali Alasmael, Noura Alkhamiss, Abdullah Fernández, Nelson Open Access Maced J Med Sci Basic Science BACKGROUND: The cluster of differentiation (CD) 74 is known for its immunological functions and its elevated level was reported in various cancer cells. AIM: The aim of the present study was to investigate the expression and potential roles of CD74 in the proliferative and apoptotic activity of breast cancer. METHODS: Expression of CD74, macrophage migration inhibitory factor (MIF) and CD44 was assayed in CAMA-1 and MDA-MB-231 cell lines using flow cytometry. CD74 was knocked down using CD74 siRNA-transfection in CAMA-1, and MDA-MB-231 cells and proliferation and apoptosis were determined in the transfected breast cancer cells. RESULTS: The data showed that CD74, MIF and CD44 were expressed in breast cancer cell lines and were associated with cell proliferation and apoptosis. Correlation analysis revealed that CD74 was positively correlated and colocalised with MIF on the cell-surface of CAMA-1 and MDA-MB-231. The knockdown of CD74 significantly reduced CAMA-1 and MDA-MB-231 cell proliferation and increased the level of apoptotic cells. CONCLUSION: We concluded that the interactions of CD74 with MIF and CD74 with CD44 could be a potential tumour marker for breast cancer cells. Moreover, the level of co-expression of MIF and CD74 or CD44 could be a surrogate marker for the efficacy of anti-angiogenic drugs, particularly in breast cancer tumours. In short, the study revealed the potential roles of CD74 in the proliferation and apoptosis of breast cancer which may serve as a potential therapeutic target for breast cancer. Republic of Macedonia 2019-10-14 /pmc/articles/PMC6953917/ /pubmed/31949511 http://dx.doi.org/10.3889/oamjms.2019.354 Text en Copyright: © 2019 Hussain Al Ssadh, Waleed Al Abdulmonem, Zafar Rasheed, Inamul Hasan Madar, Jamila Alhoderi, Samah K. Nasr Eldeen, Ali Alradhwan, Noura Alasmael, Abdullah Alkhamiss, Nelson Fernández. http://creativecommons.org/licenses/CC BY-NC/4.0 This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International License (CC BY-NC 4.0) |
spellingShingle | Basic Science Ssadh, Hussain Al Abdulmonem, Waleed Al Rasheed, Zafar Madar, Inamul Hasan Alhoderi, Jamila Eldeen, Samah K. Nasr Alradhwan, Ali Alasmael, Noura Alkhamiss, Abdullah Fernández, Nelson Knockdown of CD-74 in the Proliferative and Apoptotic Activity of Breast Cancer Cells |
title | Knockdown of CD-74 in the Proliferative and Apoptotic Activity of Breast Cancer Cells |
title_full | Knockdown of CD-74 in the Proliferative and Apoptotic Activity of Breast Cancer Cells |
title_fullStr | Knockdown of CD-74 in the Proliferative and Apoptotic Activity of Breast Cancer Cells |
title_full_unstemmed | Knockdown of CD-74 in the Proliferative and Apoptotic Activity of Breast Cancer Cells |
title_short | Knockdown of CD-74 in the Proliferative and Apoptotic Activity of Breast Cancer Cells |
title_sort | knockdown of cd-74 in the proliferative and apoptotic activity of breast cancer cells |
topic | Basic Science |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6953917/ https://www.ncbi.nlm.nih.gov/pubmed/31949511 http://dx.doi.org/10.3889/oamjms.2019.354 |
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