Cargando…

Nitric Oxide Gene Polymorphism is a Risk Factor for Diabetic Nephropathy and Atherosclerosis in Type 1 Diabetic Patients

AIM: To assess the risk factor for diabetic atherosclerosis nephropathy and diabetic nephropathy in type 1 diabetic patients. PATIENTS AND METHODS: Thirty healthy volunteers age and sex-matched and Sixty-five type 1 diabetic patient were in rolled in the study. The mean age of patients was 17.99 ± 2...

Descripción completa

Detalles Bibliográficos
Autores principales: Dayem, Soha M. Abd El, Battah, Ahmed A., Bohy, Abo El Maged El, Ahmed, Solaf, Hamed, Mona, Fattah, Safa Nabil Abd El
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Republic of Macedonia 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6953926/
https://www.ncbi.nlm.nih.gov/pubmed/31949504
http://dx.doi.org/10.3889/oamjms.2019.831
_version_ 1783486704709009408
author Dayem, Soha M. Abd El
Battah, Ahmed A.
Bohy, Abo El Maged El
Ahmed, Solaf
Hamed, Mona
Fattah, Safa Nabil Abd El
author_facet Dayem, Soha M. Abd El
Battah, Ahmed A.
Bohy, Abo El Maged El
Ahmed, Solaf
Hamed, Mona
Fattah, Safa Nabil Abd El
author_sort Dayem, Soha M. Abd El
collection PubMed
description AIM: To assess the risk factor for diabetic atherosclerosis nephropathy and diabetic nephropathy in type 1 diabetic patients. PATIENTS AND METHODS: Thirty healthy volunteers age and sex-matched and Sixty-five type 1 diabetic patient were in rolled in the study. The mean age of patients was 17.99 ± 2.59 years, mean age of onset of diabetes was 7.00 ± 3.28 years, mean duration of diabetes was 10.91 ± 3.54 years. Glycosylated sex-matched (HbA1c) was assessed in blood samples, serum lipid profile was determined, and serum level of oxidised low-density lipoprotein (OxLDL), and nitric oxide was evaluated by enzyme-linked immunosorbent assay (ELISA) technique. Nitric oxide 894G > T genotype was analysed by (PCR-RFLP) method and confirmed by Sequencing. Assessment of the albumin / creatinine ratio was done in urine samples. Renal Doppler and Carotid intima-media thickness (cIMT) via ultrasound was also performed. RESULTS: OxLDL, lipid profile, albumin/creatinine ratio, cIMT and resistivity index were significantly higher in diabetic patients while nitric oxide was significantly lower. Nitric oxide genotype shows no significant difference between diabetic’s patients and controls. Diabetic patients with homozygous NO had a significantly lower serum level of Nitric oxide, a significantly higher OxLDL, albumin / creatinine ratio and lipid profile. CONCLUSION: diabetic patients are liable for the occurrence of early diabetic nephropathy and atherosclerosis as a result of the presence of low level of nitric oxide. Nitric oxide gene polymorphism 894G > T in diabetic patients is a risk factor for diabetic nephropathy and atherosclerosis.
format Online
Article
Text
id pubmed-6953926
institution National Center for Biotechnology Information
language English
publishDate 2019
publisher Republic of Macedonia
record_format MEDLINE/PubMed
spelling pubmed-69539262020-01-16 Nitric Oxide Gene Polymorphism is a Risk Factor for Diabetic Nephropathy and Atherosclerosis in Type 1 Diabetic Patients Dayem, Soha M. Abd El Battah, Ahmed A. Bohy, Abo El Maged El Ahmed, Solaf Hamed, Mona Fattah, Safa Nabil Abd El Open Access Maced J Med Sci Basic Science AIM: To assess the risk factor for diabetic atherosclerosis nephropathy and diabetic nephropathy in type 1 diabetic patients. PATIENTS AND METHODS: Thirty healthy volunteers age and sex-matched and Sixty-five type 1 diabetic patient were in rolled in the study. The mean age of patients was 17.99 ± 2.59 years, mean age of onset of diabetes was 7.00 ± 3.28 years, mean duration of diabetes was 10.91 ± 3.54 years. Glycosylated sex-matched (HbA1c) was assessed in blood samples, serum lipid profile was determined, and serum level of oxidised low-density lipoprotein (OxLDL), and nitric oxide was evaluated by enzyme-linked immunosorbent assay (ELISA) technique. Nitric oxide 894G > T genotype was analysed by (PCR-RFLP) method and confirmed by Sequencing. Assessment of the albumin / creatinine ratio was done in urine samples. Renal Doppler and Carotid intima-media thickness (cIMT) via ultrasound was also performed. RESULTS: OxLDL, lipid profile, albumin/creatinine ratio, cIMT and resistivity index were significantly higher in diabetic patients while nitric oxide was significantly lower. Nitric oxide genotype shows no significant difference between diabetic’s patients and controls. Diabetic patients with homozygous NO had a significantly lower serum level of Nitric oxide, a significantly higher OxLDL, albumin / creatinine ratio and lipid profile. CONCLUSION: diabetic patients are liable for the occurrence of early diabetic nephropathy and atherosclerosis as a result of the presence of low level of nitric oxide. Nitric oxide gene polymorphism 894G > T in diabetic patients is a risk factor for diabetic nephropathy and atherosclerosis. Republic of Macedonia 2019-10-14 /pmc/articles/PMC6953926/ /pubmed/31949504 http://dx.doi.org/10.3889/oamjms.2019.831 Text en Copyright: © 2019 Soha M. Abd El Dayem, Ahmed A. Battah, Abo El Maged El Bohy, Solaf Ahmed, Mona Hamed, Safa Nabil Abd El Fattah. http://creativecommons.org/licenses/CC BY-NC/4.0 This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International License (CC BY-NC 4.0)
spellingShingle Basic Science
Dayem, Soha M. Abd El
Battah, Ahmed A.
Bohy, Abo El Maged El
Ahmed, Solaf
Hamed, Mona
Fattah, Safa Nabil Abd El
Nitric Oxide Gene Polymorphism is a Risk Factor for Diabetic Nephropathy and Atherosclerosis in Type 1 Diabetic Patients
title Nitric Oxide Gene Polymorphism is a Risk Factor for Diabetic Nephropathy and Atherosclerosis in Type 1 Diabetic Patients
title_full Nitric Oxide Gene Polymorphism is a Risk Factor for Diabetic Nephropathy and Atherosclerosis in Type 1 Diabetic Patients
title_fullStr Nitric Oxide Gene Polymorphism is a Risk Factor for Diabetic Nephropathy and Atherosclerosis in Type 1 Diabetic Patients
title_full_unstemmed Nitric Oxide Gene Polymorphism is a Risk Factor for Diabetic Nephropathy and Atherosclerosis in Type 1 Diabetic Patients
title_short Nitric Oxide Gene Polymorphism is a Risk Factor for Diabetic Nephropathy and Atherosclerosis in Type 1 Diabetic Patients
title_sort nitric oxide gene polymorphism is a risk factor for diabetic nephropathy and atherosclerosis in type 1 diabetic patients
topic Basic Science
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6953926/
https://www.ncbi.nlm.nih.gov/pubmed/31949504
http://dx.doi.org/10.3889/oamjms.2019.831
work_keys_str_mv AT dayemsohamabdel nitricoxidegenepolymorphismisariskfactorfordiabeticnephropathyandatherosclerosisintype1diabeticpatients
AT battahahmeda nitricoxidegenepolymorphismisariskfactorfordiabeticnephropathyandatherosclerosisintype1diabeticpatients
AT bohyaboelmagedel nitricoxidegenepolymorphismisariskfactorfordiabeticnephropathyandatherosclerosisintype1diabeticpatients
AT ahmedsolaf nitricoxidegenepolymorphismisariskfactorfordiabeticnephropathyandatherosclerosisintype1diabeticpatients
AT hamedmona nitricoxidegenepolymorphismisariskfactorfordiabeticnephropathyandatherosclerosisintype1diabeticpatients
AT fattahsafanabilabdel nitricoxidegenepolymorphismisariskfactorfordiabeticnephropathyandatherosclerosisintype1diabeticpatients