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Calcite incorporated in silica/collagen xerogels mediates calcium release and enhances osteoblast proliferation and differentiation

Multiphasic silica/collagen xerogels are biomaterials designed for bone regeneration. Biphasic silica/collagen xerogels (B30) and triphasic xerogels (B30H20 or B30CK20) additionally containing hydroxyapatite or calcite were demonstrated to exhibit several structural levels. On the first level, low f...

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Autores principales: Rößler, S., Unbehau, R., Gemming, T., Kruppke, B., Wiesmann, H.-P., Hanke, T.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6954176/
https://www.ncbi.nlm.nih.gov/pubmed/31924823
http://dx.doi.org/10.1038/s41598-019-56023-8
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author Rößler, S.
Unbehau, R.
Gemming, T.
Kruppke, B.
Wiesmann, H.-P.
Hanke, T.
author_facet Rößler, S.
Unbehau, R.
Gemming, T.
Kruppke, B.
Wiesmann, H.-P.
Hanke, T.
author_sort Rößler, S.
collection PubMed
description Multiphasic silica/collagen xerogels are biomaterials designed for bone regeneration. Biphasic silica/collagen xerogels (B30) and triphasic xerogels (B30H20 or B30CK20) additionally containing hydroxyapatite or calcite were demonstrated to exhibit several structural levels. On the first level, low fibrillar collagen serves as template for silica nanoparticle agglomerates. On second level, this silica-enriched matrix phase is fiber-reinforced by collagen fibrils. In case of hydroxyapatite incorporation in B30H20, resulting xerogels exhibit a hydroxyapatite-enriched phase consisting of hydroxyapatite particle agglomerates next to silica and low fibrillar collagen. Calcite in B30CK20 is incorporated as single non-agglomerated crystal into the silica/collagen matrix phase with embedded collagen fibrils. Both the structure of multiphasic xerogels and the manner of hydroxyapatite or calcite incorporation have an influence on the release of calcium from the xerogels. B30CK20 released a significantly higher amount of calcium into a calcium-free solution over a three-week period than B30H20. In calcium containing incubation media, all xerogels caused a decrease in calcium concentration as a result of their bioactivity, which was superimposed by the calcium release for B30CK20 and B30H20. Proliferation of human bone marrow stromal cells in direct contact to the materials was enhanced on B30CK20 compared to cells on both plain B30 and B30H20.
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spelling pubmed-69541762020-01-15 Calcite incorporated in silica/collagen xerogels mediates calcium release and enhances osteoblast proliferation and differentiation Rößler, S. Unbehau, R. Gemming, T. Kruppke, B. Wiesmann, H.-P. Hanke, T. Sci Rep Article Multiphasic silica/collagen xerogels are biomaterials designed for bone regeneration. Biphasic silica/collagen xerogels (B30) and triphasic xerogels (B30H20 or B30CK20) additionally containing hydroxyapatite or calcite were demonstrated to exhibit several structural levels. On the first level, low fibrillar collagen serves as template for silica nanoparticle agglomerates. On second level, this silica-enriched matrix phase is fiber-reinforced by collagen fibrils. In case of hydroxyapatite incorporation in B30H20, resulting xerogels exhibit a hydroxyapatite-enriched phase consisting of hydroxyapatite particle agglomerates next to silica and low fibrillar collagen. Calcite in B30CK20 is incorporated as single non-agglomerated crystal into the silica/collagen matrix phase with embedded collagen fibrils. Both the structure of multiphasic xerogels and the manner of hydroxyapatite or calcite incorporation have an influence on the release of calcium from the xerogels. B30CK20 released a significantly higher amount of calcium into a calcium-free solution over a three-week period than B30H20. In calcium containing incubation media, all xerogels caused a decrease in calcium concentration as a result of their bioactivity, which was superimposed by the calcium release for B30CK20 and B30H20. Proliferation of human bone marrow stromal cells in direct contact to the materials was enhanced on B30CK20 compared to cells on both plain B30 and B30H20. Nature Publishing Group UK 2020-01-10 /pmc/articles/PMC6954176/ /pubmed/31924823 http://dx.doi.org/10.1038/s41598-019-56023-8 Text en © The Author(s) 2020 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
Rößler, S.
Unbehau, R.
Gemming, T.
Kruppke, B.
Wiesmann, H.-P.
Hanke, T.
Calcite incorporated in silica/collagen xerogels mediates calcium release and enhances osteoblast proliferation and differentiation
title Calcite incorporated in silica/collagen xerogels mediates calcium release and enhances osteoblast proliferation and differentiation
title_full Calcite incorporated in silica/collagen xerogels mediates calcium release and enhances osteoblast proliferation and differentiation
title_fullStr Calcite incorporated in silica/collagen xerogels mediates calcium release and enhances osteoblast proliferation and differentiation
title_full_unstemmed Calcite incorporated in silica/collagen xerogels mediates calcium release and enhances osteoblast proliferation and differentiation
title_short Calcite incorporated in silica/collagen xerogels mediates calcium release and enhances osteoblast proliferation and differentiation
title_sort calcite incorporated in silica/collagen xerogels mediates calcium release and enhances osteoblast proliferation and differentiation
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6954176/
https://www.ncbi.nlm.nih.gov/pubmed/31924823
http://dx.doi.org/10.1038/s41598-019-56023-8
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