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Calcite incorporated in silica/collagen xerogels mediates calcium release and enhances osteoblast proliferation and differentiation
Multiphasic silica/collagen xerogels are biomaterials designed for bone regeneration. Biphasic silica/collagen xerogels (B30) and triphasic xerogels (B30H20 or B30CK20) additionally containing hydroxyapatite or calcite were demonstrated to exhibit several structural levels. On the first level, low f...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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Nature Publishing Group UK
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6954176/ https://www.ncbi.nlm.nih.gov/pubmed/31924823 http://dx.doi.org/10.1038/s41598-019-56023-8 |
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author | Rößler, S. Unbehau, R. Gemming, T. Kruppke, B. Wiesmann, H.-P. Hanke, T. |
author_facet | Rößler, S. Unbehau, R. Gemming, T. Kruppke, B. Wiesmann, H.-P. Hanke, T. |
author_sort | Rößler, S. |
collection | PubMed |
description | Multiphasic silica/collagen xerogels are biomaterials designed for bone regeneration. Biphasic silica/collagen xerogels (B30) and triphasic xerogels (B30H20 or B30CK20) additionally containing hydroxyapatite or calcite were demonstrated to exhibit several structural levels. On the first level, low fibrillar collagen serves as template for silica nanoparticle agglomerates. On second level, this silica-enriched matrix phase is fiber-reinforced by collagen fibrils. In case of hydroxyapatite incorporation in B30H20, resulting xerogels exhibit a hydroxyapatite-enriched phase consisting of hydroxyapatite particle agglomerates next to silica and low fibrillar collagen. Calcite in B30CK20 is incorporated as single non-agglomerated crystal into the silica/collagen matrix phase with embedded collagen fibrils. Both the structure of multiphasic xerogels and the manner of hydroxyapatite or calcite incorporation have an influence on the release of calcium from the xerogels. B30CK20 released a significantly higher amount of calcium into a calcium-free solution over a three-week period than B30H20. In calcium containing incubation media, all xerogels caused a decrease in calcium concentration as a result of their bioactivity, which was superimposed by the calcium release for B30CK20 and B30H20. Proliferation of human bone marrow stromal cells in direct contact to the materials was enhanced on B30CK20 compared to cells on both plain B30 and B30H20. |
format | Online Article Text |
id | pubmed-6954176 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | Nature Publishing Group UK |
record_format | MEDLINE/PubMed |
spelling | pubmed-69541762020-01-15 Calcite incorporated in silica/collagen xerogels mediates calcium release and enhances osteoblast proliferation and differentiation Rößler, S. Unbehau, R. Gemming, T. Kruppke, B. Wiesmann, H.-P. Hanke, T. Sci Rep Article Multiphasic silica/collagen xerogels are biomaterials designed for bone regeneration. Biphasic silica/collagen xerogels (B30) and triphasic xerogels (B30H20 or B30CK20) additionally containing hydroxyapatite or calcite were demonstrated to exhibit several structural levels. On the first level, low fibrillar collagen serves as template for silica nanoparticle agglomerates. On second level, this silica-enriched matrix phase is fiber-reinforced by collagen fibrils. In case of hydroxyapatite incorporation in B30H20, resulting xerogels exhibit a hydroxyapatite-enriched phase consisting of hydroxyapatite particle agglomerates next to silica and low fibrillar collagen. Calcite in B30CK20 is incorporated as single non-agglomerated crystal into the silica/collagen matrix phase with embedded collagen fibrils. Both the structure of multiphasic xerogels and the manner of hydroxyapatite or calcite incorporation have an influence on the release of calcium from the xerogels. B30CK20 released a significantly higher amount of calcium into a calcium-free solution over a three-week period than B30H20. In calcium containing incubation media, all xerogels caused a decrease in calcium concentration as a result of their bioactivity, which was superimposed by the calcium release for B30CK20 and B30H20. Proliferation of human bone marrow stromal cells in direct contact to the materials was enhanced on B30CK20 compared to cells on both plain B30 and B30H20. Nature Publishing Group UK 2020-01-10 /pmc/articles/PMC6954176/ /pubmed/31924823 http://dx.doi.org/10.1038/s41598-019-56023-8 Text en © The Author(s) 2020 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/. |
spellingShingle | Article Rößler, S. Unbehau, R. Gemming, T. Kruppke, B. Wiesmann, H.-P. Hanke, T. Calcite incorporated in silica/collagen xerogels mediates calcium release and enhances osteoblast proliferation and differentiation |
title | Calcite incorporated in silica/collagen xerogels mediates calcium release and enhances osteoblast proliferation and differentiation |
title_full | Calcite incorporated in silica/collagen xerogels mediates calcium release and enhances osteoblast proliferation and differentiation |
title_fullStr | Calcite incorporated in silica/collagen xerogels mediates calcium release and enhances osteoblast proliferation and differentiation |
title_full_unstemmed | Calcite incorporated in silica/collagen xerogels mediates calcium release and enhances osteoblast proliferation and differentiation |
title_short | Calcite incorporated in silica/collagen xerogels mediates calcium release and enhances osteoblast proliferation and differentiation |
title_sort | calcite incorporated in silica/collagen xerogels mediates calcium release and enhances osteoblast proliferation and differentiation |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6954176/ https://www.ncbi.nlm.nih.gov/pubmed/31924823 http://dx.doi.org/10.1038/s41598-019-56023-8 |
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