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Non-invasive optical control of endogenous Ca(2+) channels in awake mice

Optogenetic approaches for controlling Ca(2+) channels provide powerful means for modulating diverse Ca(2+)-specific biological events in space and time. However, blue light-responsive photoreceptors are, in principle, considered inadequate for deep tissue stimulation unless accompanied by optic fib...

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Detalles Bibliográficos
Autores principales: Kim, Sungsoo, Kyung, Taeyoon, Chung, Jae-Hee, Kim, Nury, Keum, Sehoon, Lee, Jinsu, Park, Hyerim, Kim, Ho Min, Lee, Sangkyu, Shin, Hee-Sup, Heo, Won Do
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6954201/
https://www.ncbi.nlm.nih.gov/pubmed/31924789
http://dx.doi.org/10.1038/s41467-019-14005-4
Descripción
Sumario:Optogenetic approaches for controlling Ca(2+) channels provide powerful means for modulating diverse Ca(2+)-specific biological events in space and time. However, blue light-responsive photoreceptors are, in principle, considered inadequate for deep tissue stimulation unless accompanied by optic fiber insertion. Here, we present an ultra-light-sensitive optogenetic Ca(2+) modulator, named monSTIM1 encompassing engineered cryptochrome2 for manipulating Ca(2+) signaling in the brain of awake mice through non-invasive light delivery. Activation of monSTIM1 in either excitatory neurons or astrocytes of mice brain is able to induce Ca(2+)-dependent gene expression without any mechanical damage in the brain. Furthermore, we demonstrate that non-invasive Ca(2+) modulation in neurons can be sufficiently and effectively translated into changes in behavioral phenotypes of awake mice.