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Label-free impedance flow cytometry for nanotoxicity screening
The development of reliable and cost-efficient methods to assess the toxicity of nanomaterials (NMs) is critical for the proper identification of their impact on human health and for ensuring a safe progress of nanotechnology. In this study, we investigated the reliability and applicability of label...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group UK
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6954202/ https://www.ncbi.nlm.nih.gov/pubmed/31924828 http://dx.doi.org/10.1038/s41598-019-56705-3 |
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author | Ostermann, Melanie Sauter, Alexander Xue, Ying Birkeland, Eivind Schoelermann, Julia Holst, Bodil Cimpan, Mihaela Roxana |
author_facet | Ostermann, Melanie Sauter, Alexander Xue, Ying Birkeland, Eivind Schoelermann, Julia Holst, Bodil Cimpan, Mihaela Roxana |
author_sort | Ostermann, Melanie |
collection | PubMed |
description | The development of reliable and cost-efficient methods to assess the toxicity of nanomaterials (NMs) is critical for the proper identification of their impact on human health and for ensuring a safe progress of nanotechnology. In this study, we investigated the reliability and applicability of label-free impedance flow cytometry (IFC) for in vitro nanotoxicity screening, which avoids time-consuming labelling steps and minimizes possible NM-induced interferences. U937 human lymphoma cells were exposed for 24 h to eight different nanomaterials at five concentrations (2, 10, 20, 50, and 100 μg/mL). The NMs’ effect on viability was measured using IFC and the results were compared to those obtained by trypan blue (TB) dye exclusion and conventional flow cytometry (FC). To discriminate viable from necrotic cells, the IFC measurement settings regarding signal trigger level and frequency, as well as the buffer composition, were optimised. A clear discrimination between viable and necrotic cells was obtained at 6 MHz in a sucrose-based measurement buffer. Nanomaterial-induced interferences were not detected for IFC. The IFC and TB assay results were in accordance for all NMs. The IFC was found to be robust, reliable and less prone to interferences due to the advantage of being label-free. |
format | Online Article Text |
id | pubmed-6954202 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | Nature Publishing Group UK |
record_format | MEDLINE/PubMed |
spelling | pubmed-69542022020-01-15 Label-free impedance flow cytometry for nanotoxicity screening Ostermann, Melanie Sauter, Alexander Xue, Ying Birkeland, Eivind Schoelermann, Julia Holst, Bodil Cimpan, Mihaela Roxana Sci Rep Article The development of reliable and cost-efficient methods to assess the toxicity of nanomaterials (NMs) is critical for the proper identification of their impact on human health and for ensuring a safe progress of nanotechnology. In this study, we investigated the reliability and applicability of label-free impedance flow cytometry (IFC) for in vitro nanotoxicity screening, which avoids time-consuming labelling steps and minimizes possible NM-induced interferences. U937 human lymphoma cells were exposed for 24 h to eight different nanomaterials at five concentrations (2, 10, 20, 50, and 100 μg/mL). The NMs’ effect on viability was measured using IFC and the results were compared to those obtained by trypan blue (TB) dye exclusion and conventional flow cytometry (FC). To discriminate viable from necrotic cells, the IFC measurement settings regarding signal trigger level and frequency, as well as the buffer composition, were optimised. A clear discrimination between viable and necrotic cells was obtained at 6 MHz in a sucrose-based measurement buffer. Nanomaterial-induced interferences were not detected for IFC. The IFC and TB assay results were in accordance for all NMs. The IFC was found to be robust, reliable and less prone to interferences due to the advantage of being label-free. Nature Publishing Group UK 2020-01-10 /pmc/articles/PMC6954202/ /pubmed/31924828 http://dx.doi.org/10.1038/s41598-019-56705-3 Text en © The Author(s) 2020 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/. |
spellingShingle | Article Ostermann, Melanie Sauter, Alexander Xue, Ying Birkeland, Eivind Schoelermann, Julia Holst, Bodil Cimpan, Mihaela Roxana Label-free impedance flow cytometry for nanotoxicity screening |
title | Label-free impedance flow cytometry for nanotoxicity screening |
title_full | Label-free impedance flow cytometry for nanotoxicity screening |
title_fullStr | Label-free impedance flow cytometry for nanotoxicity screening |
title_full_unstemmed | Label-free impedance flow cytometry for nanotoxicity screening |
title_short | Label-free impedance flow cytometry for nanotoxicity screening |
title_sort | label-free impedance flow cytometry for nanotoxicity screening |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6954202/ https://www.ncbi.nlm.nih.gov/pubmed/31924828 http://dx.doi.org/10.1038/s41598-019-56705-3 |
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