An electrochemical biosensor for the detection of epithelial-mesenchymal transition

Epithelial-mesenchymal transition (EMT) is critically involved in a variety of biological processes. Electrochemical sensing offers potential to develop more effective technology for EMT detection. In this study, by using the unique performance of quantum dot (QD)-nanocomposite materials, we establi...

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Autores principales: Du, Xin, Zhang, Zhenhua, Zheng, Xiaodi, Zhang, Hongyan, Dong, Dan, Zhang, Zhenguo, Liu, Min, Zhou, Jun
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2020
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6954246/
https://www.ncbi.nlm.nih.gov/pubmed/31924791
http://dx.doi.org/10.1038/s41467-019-14037-w
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author Du, Xin
Zhang, Zhenhua
Zheng, Xiaodi
Zhang, Hongyan
Dong, Dan
Zhang, Zhenguo
Liu, Min
Zhou, Jun
author_facet Du, Xin
Zhang, Zhenhua
Zheng, Xiaodi
Zhang, Hongyan
Dong, Dan
Zhang, Zhenguo
Liu, Min
Zhou, Jun
author_sort Du, Xin
collection PubMed
description Epithelial-mesenchymal transition (EMT) is critically involved in a variety of biological processes. Electrochemical sensing offers potential to develop more effective technology for EMT detection. In this study, by using the unique performance of quantum dot (QD)-nanocomposite materials, we establish an electrochemical biosensor that can specifically detect the change of E-cadherin and analyze different stages of EMT. The signal for EMT is largely magnified due to the transmission of molecular information to the electronic device. In addition, differential pulse voltammetry reveals that the response of the electrochemical signals is rapid and sensitive, due to the synergistic effect of QDs and carbon nanotube-gold nanoparticles. Our study thus suggests that electrochemical sensing is an effective technology for detecting EMT and may have broad applications in analyzing various cell type transitions.
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spelling pubmed-69542462020-01-13 An electrochemical biosensor for the detection of epithelial-mesenchymal transition Du, Xin Zhang, Zhenhua Zheng, Xiaodi Zhang, Hongyan Dong, Dan Zhang, Zhenguo Liu, Min Zhou, Jun Nat Commun Article Epithelial-mesenchymal transition (EMT) is critically involved in a variety of biological processes. Electrochemical sensing offers potential to develop more effective technology for EMT detection. In this study, by using the unique performance of quantum dot (QD)-nanocomposite materials, we establish an electrochemical biosensor that can specifically detect the change of E-cadherin and analyze different stages of EMT. The signal for EMT is largely magnified due to the transmission of molecular information to the electronic device. In addition, differential pulse voltammetry reveals that the response of the electrochemical signals is rapid and sensitive, due to the synergistic effect of QDs and carbon nanotube-gold nanoparticles. Our study thus suggests that electrochemical sensing is an effective technology for detecting EMT and may have broad applications in analyzing various cell type transitions. Nature Publishing Group UK 2020-01-10 /pmc/articles/PMC6954246/ /pubmed/31924791 http://dx.doi.org/10.1038/s41467-019-14037-w Text en © The Author(s) 2020 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
Du, Xin
Zhang, Zhenhua
Zheng, Xiaodi
Zhang, Hongyan
Dong, Dan
Zhang, Zhenguo
Liu, Min
Zhou, Jun
An electrochemical biosensor for the detection of epithelial-mesenchymal transition
title An electrochemical biosensor for the detection of epithelial-mesenchymal transition
title_full An electrochemical biosensor for the detection of epithelial-mesenchymal transition
title_fullStr An electrochemical biosensor for the detection of epithelial-mesenchymal transition
title_full_unstemmed An electrochemical biosensor for the detection of epithelial-mesenchymal transition
title_short An electrochemical biosensor for the detection of epithelial-mesenchymal transition
title_sort electrochemical biosensor for the detection of epithelial-mesenchymal transition
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6954246/
https://www.ncbi.nlm.nih.gov/pubmed/31924791
http://dx.doi.org/10.1038/s41467-019-14037-w
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