Expanding the editable genome and CRISPR–Cas9 versatility using DNA cutting-free gene targeting based on in trans paired nicking

Genome editing typically involves recombination between donor nucleic acids and acceptor genomic sequences subjected to double-stranded DNA breaks (DSBs) made by programmable nucleases (e.g. CRISPR–Cas9). Yet, nucleases yield off-target mutations and, most pervasively, unpredictable target allele di...

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Detalles Bibliográficos
Autores principales: Chen, Xiaoyu, Tasca, Francesca, Wang, Qian, Liu, Jin, Janssen, Josephine M, Brescia, Marcella D, Bellin, Milena, Szuhai, Karoly, Kenrick, Josefin, Frock, Richard L, Gonçalves, Manuel A F V
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Oxford University Press 2020
Materias:
Synthetic Biology and Bioengineering
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6954423/
https://www.ncbi.nlm.nih.gov/pubmed/31799604
http://dx.doi.org/10.1093/nar/gkz1121

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