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Mapping RNA–capsid interactions and RNA secondary structure within virus particles using next-generation sequencing

To characterize RNA–capsid binding sites genome-wide within mature RNA virus particles, we have developed a Next-Generation Sequencing (NGS) platform: viral Photo-Activatable Ribonucleoside CrossLinking (vPAR-CL). In vPAR-CL, 4-thiouridine is incorporated into the encapsidated genomes of virus parti...

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Detalles Bibliográficos
Autores principales:	Zhou, Yiyang, Routh, Andrew
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Oxford University Press 2020
Materias:	Methods Online
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6954446/ https://www.ncbi.nlm.nih.gov/pubmed/31799606 http://dx.doi.org/10.1093/nar/gkz1124

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