Cargando…
Cytotoxic Potential of the Coelomic Fluid Extracted from the Sea Cucumber Holothuria tubulosa against Triple-Negative MDA-MB231 Breast Cancer Cells
Growing evidence has demonstrated that the extracts of different holothurian species exert beneficial effects on human health. Triple negative breast cancers (TNBC) are highly malignant tumors that present a poor prognosis due to the lack of effective targeted therapies. In the attempt to identify n...
Autores principales: | , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2019
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6955747/ https://www.ncbi.nlm.nih.gov/pubmed/31600896 http://dx.doi.org/10.3390/biology8040076 |
_version_ | 1783486999561240576 |
---|---|
author | Luparello, Claudio Ragona, Debora Asaro, Dalia Maria Lucia Lazzara, Valentina Affranchi, Federica Celi, Monica Arizza, Vincenzo Vazzana, Mirella |
author_facet | Luparello, Claudio Ragona, Debora Asaro, Dalia Maria Lucia Lazzara, Valentina Affranchi, Federica Celi, Monica Arizza, Vincenzo Vazzana, Mirella |
author_sort | Luparello, Claudio |
collection | PubMed |
description | Growing evidence has demonstrated that the extracts of different holothurian species exert beneficial effects on human health. Triple negative breast cancers (TNBC) are highly malignant tumors that present a poor prognosis due to the lack of effective targeted therapies. In the attempt to identify novel compounds that might counteract TNBC cell growth, we studied the effect of the exposure of the TNBC cell line MDA-MB231 to total and filtered aqueous extracts of the coelomic fluid obtained from the sea cucumber Holoturia tubulosa, a widespread species in the Mediterranean Sea. In particular, we examined cell viability and proliferative behaviour, cell cycle distribution, apoptosis, autophagy, and mitochondrial metabolic/cell redox state. The results obtained indicate that both total and fractionated extracts are potent inhibitors of TNBC cell viability and growth, acting through both an impairment of cell cycle progression and mitochondrial transmembrane potential and a stimulation of cellular autophagy, as demonstrated by the increase of the acidic vesicular organelles and of the intracellular protein markers beclin-1, and total LC3 and LC3-II upon early exposure to the preparations. Identification of the water-soluble bioactive component(s) present in the extract merit further investigation aiming to develop novel prevention and/or treatment agents efficacious against highly metastatic breast carcinomas. |
format | Online Article Text |
id | pubmed-6955747 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-69557472020-01-23 Cytotoxic Potential of the Coelomic Fluid Extracted from the Sea Cucumber Holothuria tubulosa against Triple-Negative MDA-MB231 Breast Cancer Cells Luparello, Claudio Ragona, Debora Asaro, Dalia Maria Lucia Lazzara, Valentina Affranchi, Federica Celi, Monica Arizza, Vincenzo Vazzana, Mirella Biology (Basel) Article Growing evidence has demonstrated that the extracts of different holothurian species exert beneficial effects on human health. Triple negative breast cancers (TNBC) are highly malignant tumors that present a poor prognosis due to the lack of effective targeted therapies. In the attempt to identify novel compounds that might counteract TNBC cell growth, we studied the effect of the exposure of the TNBC cell line MDA-MB231 to total and filtered aqueous extracts of the coelomic fluid obtained from the sea cucumber Holoturia tubulosa, a widespread species in the Mediterranean Sea. In particular, we examined cell viability and proliferative behaviour, cell cycle distribution, apoptosis, autophagy, and mitochondrial metabolic/cell redox state. The results obtained indicate that both total and fractionated extracts are potent inhibitors of TNBC cell viability and growth, acting through both an impairment of cell cycle progression and mitochondrial transmembrane potential and a stimulation of cellular autophagy, as demonstrated by the increase of the acidic vesicular organelles and of the intracellular protein markers beclin-1, and total LC3 and LC3-II upon early exposure to the preparations. Identification of the water-soluble bioactive component(s) present in the extract merit further investigation aiming to develop novel prevention and/or treatment agents efficacious against highly metastatic breast carcinomas. MDPI 2019-10-09 /pmc/articles/PMC6955747/ /pubmed/31600896 http://dx.doi.org/10.3390/biology8040076 Text en © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Luparello, Claudio Ragona, Debora Asaro, Dalia Maria Lucia Lazzara, Valentina Affranchi, Federica Celi, Monica Arizza, Vincenzo Vazzana, Mirella Cytotoxic Potential of the Coelomic Fluid Extracted from the Sea Cucumber Holothuria tubulosa against Triple-Negative MDA-MB231 Breast Cancer Cells |
title | Cytotoxic Potential of the Coelomic Fluid Extracted from the Sea Cucumber Holothuria tubulosa against Triple-Negative MDA-MB231 Breast Cancer Cells |
title_full | Cytotoxic Potential of the Coelomic Fluid Extracted from the Sea Cucumber Holothuria tubulosa against Triple-Negative MDA-MB231 Breast Cancer Cells |
title_fullStr | Cytotoxic Potential of the Coelomic Fluid Extracted from the Sea Cucumber Holothuria tubulosa against Triple-Negative MDA-MB231 Breast Cancer Cells |
title_full_unstemmed | Cytotoxic Potential of the Coelomic Fluid Extracted from the Sea Cucumber Holothuria tubulosa against Triple-Negative MDA-MB231 Breast Cancer Cells |
title_short | Cytotoxic Potential of the Coelomic Fluid Extracted from the Sea Cucumber Holothuria tubulosa against Triple-Negative MDA-MB231 Breast Cancer Cells |
title_sort | cytotoxic potential of the coelomic fluid extracted from the sea cucumber holothuria tubulosa against triple-negative mda-mb231 breast cancer cells |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6955747/ https://www.ncbi.nlm.nih.gov/pubmed/31600896 http://dx.doi.org/10.3390/biology8040076 |
work_keys_str_mv | AT luparelloclaudio cytotoxicpotentialofthecoelomicfluidextractedfromtheseacucumberholothuriatubulosaagainsttriplenegativemdamb231breastcancercells AT ragonadebora cytotoxicpotentialofthecoelomicfluidextractedfromtheseacucumberholothuriatubulosaagainsttriplenegativemdamb231breastcancercells AT asarodaliamarialucia cytotoxicpotentialofthecoelomicfluidextractedfromtheseacucumberholothuriatubulosaagainsttriplenegativemdamb231breastcancercells AT lazzaravalentina cytotoxicpotentialofthecoelomicfluidextractedfromtheseacucumberholothuriatubulosaagainsttriplenegativemdamb231breastcancercells AT affranchifederica cytotoxicpotentialofthecoelomicfluidextractedfromtheseacucumberholothuriatubulosaagainsttriplenegativemdamb231breastcancercells AT celimonica cytotoxicpotentialofthecoelomicfluidextractedfromtheseacucumberholothuriatubulosaagainsttriplenegativemdamb231breastcancercells AT arizzavincenzo cytotoxicpotentialofthecoelomicfluidextractedfromtheseacucumberholothuriatubulosaagainsttriplenegativemdamb231breastcancercells AT vazzanamirella cytotoxicpotentialofthecoelomicfluidextractedfromtheseacucumberholothuriatubulosaagainsttriplenegativemdamb231breastcancercells |