Cargando…

Cytotoxic Potential of the Coelomic Fluid Extracted from the Sea Cucumber Holothuria tubulosa against Triple-Negative MDA-MB231 Breast Cancer Cells

Growing evidence has demonstrated that the extracts of different holothurian species exert beneficial effects on human health. Triple negative breast cancers (TNBC) are highly malignant tumors that present a poor prognosis due to the lack of effective targeted therapies. In the attempt to identify n...

Descripción completa

Detalles Bibliográficos
Autores principales: Luparello, Claudio, Ragona, Debora, Asaro, Dalia Maria Lucia, Lazzara, Valentina, Affranchi, Federica, Celi, Monica, Arizza, Vincenzo, Vazzana, Mirella
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6955747/
https://www.ncbi.nlm.nih.gov/pubmed/31600896
http://dx.doi.org/10.3390/biology8040076
_version_ 1783486999561240576
author Luparello, Claudio
Ragona, Debora
Asaro, Dalia Maria Lucia
Lazzara, Valentina
Affranchi, Federica
Celi, Monica
Arizza, Vincenzo
Vazzana, Mirella
author_facet Luparello, Claudio
Ragona, Debora
Asaro, Dalia Maria Lucia
Lazzara, Valentina
Affranchi, Federica
Celi, Monica
Arizza, Vincenzo
Vazzana, Mirella
author_sort Luparello, Claudio
collection PubMed
description Growing evidence has demonstrated that the extracts of different holothurian species exert beneficial effects on human health. Triple negative breast cancers (TNBC) are highly malignant tumors that present a poor prognosis due to the lack of effective targeted therapies. In the attempt to identify novel compounds that might counteract TNBC cell growth, we studied the effect of the exposure of the TNBC cell line MDA-MB231 to total and filtered aqueous extracts of the coelomic fluid obtained from the sea cucumber Holoturia tubulosa, a widespread species in the Mediterranean Sea. In particular, we examined cell viability and proliferative behaviour, cell cycle distribution, apoptosis, autophagy, and mitochondrial metabolic/cell redox state. The results obtained indicate that both total and fractionated extracts are potent inhibitors of TNBC cell viability and growth, acting through both an impairment of cell cycle progression and mitochondrial transmembrane potential and a stimulation of cellular autophagy, as demonstrated by the increase of the acidic vesicular organelles and of the intracellular protein markers beclin-1, and total LC3 and LC3-II upon early exposure to the preparations. Identification of the water-soluble bioactive component(s) present in the extract merit further investigation aiming to develop novel prevention and/or treatment agents efficacious against highly metastatic breast carcinomas.
format Online
Article
Text
id pubmed-6955747
institution National Center for Biotechnology Information
language English
publishDate 2019
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-69557472020-01-23 Cytotoxic Potential of the Coelomic Fluid Extracted from the Sea Cucumber Holothuria tubulosa against Triple-Negative MDA-MB231 Breast Cancer Cells Luparello, Claudio Ragona, Debora Asaro, Dalia Maria Lucia Lazzara, Valentina Affranchi, Federica Celi, Monica Arizza, Vincenzo Vazzana, Mirella Biology (Basel) Article Growing evidence has demonstrated that the extracts of different holothurian species exert beneficial effects on human health. Triple negative breast cancers (TNBC) are highly malignant tumors that present a poor prognosis due to the lack of effective targeted therapies. In the attempt to identify novel compounds that might counteract TNBC cell growth, we studied the effect of the exposure of the TNBC cell line MDA-MB231 to total and filtered aqueous extracts of the coelomic fluid obtained from the sea cucumber Holoturia tubulosa, a widespread species in the Mediterranean Sea. In particular, we examined cell viability and proliferative behaviour, cell cycle distribution, apoptosis, autophagy, and mitochondrial metabolic/cell redox state. The results obtained indicate that both total and fractionated extracts are potent inhibitors of TNBC cell viability and growth, acting through both an impairment of cell cycle progression and mitochondrial transmembrane potential and a stimulation of cellular autophagy, as demonstrated by the increase of the acidic vesicular organelles and of the intracellular protein markers beclin-1, and total LC3 and LC3-II upon early exposure to the preparations. Identification of the water-soluble bioactive component(s) present in the extract merit further investigation aiming to develop novel prevention and/or treatment agents efficacious against highly metastatic breast carcinomas. MDPI 2019-10-09 /pmc/articles/PMC6955747/ /pubmed/31600896 http://dx.doi.org/10.3390/biology8040076 Text en © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Luparello, Claudio
Ragona, Debora
Asaro, Dalia Maria Lucia
Lazzara, Valentina
Affranchi, Federica
Celi, Monica
Arizza, Vincenzo
Vazzana, Mirella
Cytotoxic Potential of the Coelomic Fluid Extracted from the Sea Cucumber Holothuria tubulosa against Triple-Negative MDA-MB231 Breast Cancer Cells
title Cytotoxic Potential of the Coelomic Fluid Extracted from the Sea Cucumber Holothuria tubulosa against Triple-Negative MDA-MB231 Breast Cancer Cells
title_full Cytotoxic Potential of the Coelomic Fluid Extracted from the Sea Cucumber Holothuria tubulosa against Triple-Negative MDA-MB231 Breast Cancer Cells
title_fullStr Cytotoxic Potential of the Coelomic Fluid Extracted from the Sea Cucumber Holothuria tubulosa against Triple-Negative MDA-MB231 Breast Cancer Cells
title_full_unstemmed Cytotoxic Potential of the Coelomic Fluid Extracted from the Sea Cucumber Holothuria tubulosa against Triple-Negative MDA-MB231 Breast Cancer Cells
title_short Cytotoxic Potential of the Coelomic Fluid Extracted from the Sea Cucumber Holothuria tubulosa against Triple-Negative MDA-MB231 Breast Cancer Cells
title_sort cytotoxic potential of the coelomic fluid extracted from the sea cucumber holothuria tubulosa against triple-negative mda-mb231 breast cancer cells
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6955747/
https://www.ncbi.nlm.nih.gov/pubmed/31600896
http://dx.doi.org/10.3390/biology8040076
work_keys_str_mv AT luparelloclaudio cytotoxicpotentialofthecoelomicfluidextractedfromtheseacucumberholothuriatubulosaagainsttriplenegativemdamb231breastcancercells
AT ragonadebora cytotoxicpotentialofthecoelomicfluidextractedfromtheseacucumberholothuriatubulosaagainsttriplenegativemdamb231breastcancercells
AT asarodaliamarialucia cytotoxicpotentialofthecoelomicfluidextractedfromtheseacucumberholothuriatubulosaagainsttriplenegativemdamb231breastcancercells
AT lazzaravalentina cytotoxicpotentialofthecoelomicfluidextractedfromtheseacucumberholothuriatubulosaagainsttriplenegativemdamb231breastcancercells
AT affranchifederica cytotoxicpotentialofthecoelomicfluidextractedfromtheseacucumberholothuriatubulosaagainsttriplenegativemdamb231breastcancercells
AT celimonica cytotoxicpotentialofthecoelomicfluidextractedfromtheseacucumberholothuriatubulosaagainsttriplenegativemdamb231breastcancercells
AT arizzavincenzo cytotoxicpotentialofthecoelomicfluidextractedfromtheseacucumberholothuriatubulosaagainsttriplenegativemdamb231breastcancercells
AT vazzanamirella cytotoxicpotentialofthecoelomicfluidextractedfromtheseacucumberholothuriatubulosaagainsttriplenegativemdamb231breastcancercells