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Grapevine Phyllosphere Community Analysis in Response to Elicitor Application against Powdery Mildew

The reduction of antimicrobial treatments and mainly the application of environmentally friendly compounds, such as resistance elicitors, is an impelling challenge to undertake more sustainable agriculture. We performed this research to study the effectiveness of non-conventional compounds in reduci...

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Autores principales: Nerva, Luca, Pagliarani, Chiara, Pugliese, Massimo, Monchiero, Matteo, Gonthier, Solène, Gullino, Maria Lodovica, Gambino, Giorgio, Chitarra, Walter
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6956034/
https://www.ncbi.nlm.nih.gov/pubmed/31817902
http://dx.doi.org/10.3390/microorganisms7120662
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author Nerva, Luca
Pagliarani, Chiara
Pugliese, Massimo
Monchiero, Matteo
Gonthier, Solène
Gullino, Maria Lodovica
Gambino, Giorgio
Chitarra, Walter
author_facet Nerva, Luca
Pagliarani, Chiara
Pugliese, Massimo
Monchiero, Matteo
Gonthier, Solène
Gullino, Maria Lodovica
Gambino, Giorgio
Chitarra, Walter
author_sort Nerva, Luca
collection PubMed
description The reduction of antimicrobial treatments and mainly the application of environmentally friendly compounds, such as resistance elicitors, is an impelling challenge to undertake more sustainable agriculture. We performed this research to study the effectiveness of non-conventional compounds in reducing leaf fungal attack and to investigate whether they influence the grape phyllosphere. Pathogenicity tests were conducted on potted Vitis vinifera “Nebbiolo” and “Moscato” cultivars infected with the powdery mildew agent (Erysiphe necator) and treated with three elicitors. Differences in the foliar microbial community were then evaluated by community-level physiological profiling by using Biolog(TM) EcoPlates, high throughput sequencing of the Internal Transcribed Spacer (ITS) region, and RNA sequencing for the viral community. In both cultivars, all products were effective as they significantly reduced pathogen development. EcoPlate analysis and ITS sequencing showed that the microbial communities were not influenced by the alternative compound application, confirming their specific activity as plant defense elicitors. Nevertheless, “Moscato” plants were less susceptible to the disease and presented different phyllosphere composition, resulting in a richer viral community, when compared with the “Nebbiolo” plants. The observed effect on microbial communities pointed to the existence of distinct genotype-specific defense mechanisms independently of the elicitor application.
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spelling pubmed-69560342020-01-23 Grapevine Phyllosphere Community Analysis in Response to Elicitor Application against Powdery Mildew Nerva, Luca Pagliarani, Chiara Pugliese, Massimo Monchiero, Matteo Gonthier, Solène Gullino, Maria Lodovica Gambino, Giorgio Chitarra, Walter Microorganisms Article The reduction of antimicrobial treatments and mainly the application of environmentally friendly compounds, such as resistance elicitors, is an impelling challenge to undertake more sustainable agriculture. We performed this research to study the effectiveness of non-conventional compounds in reducing leaf fungal attack and to investigate whether they influence the grape phyllosphere. Pathogenicity tests were conducted on potted Vitis vinifera “Nebbiolo” and “Moscato” cultivars infected with the powdery mildew agent (Erysiphe necator) and treated with three elicitors. Differences in the foliar microbial community were then evaluated by community-level physiological profiling by using Biolog(TM) EcoPlates, high throughput sequencing of the Internal Transcribed Spacer (ITS) region, and RNA sequencing for the viral community. In both cultivars, all products were effective as they significantly reduced pathogen development. EcoPlate analysis and ITS sequencing showed that the microbial communities were not influenced by the alternative compound application, confirming their specific activity as plant defense elicitors. Nevertheless, “Moscato” plants were less susceptible to the disease and presented different phyllosphere composition, resulting in a richer viral community, when compared with the “Nebbiolo” plants. The observed effect on microbial communities pointed to the existence of distinct genotype-specific defense mechanisms independently of the elicitor application. MDPI 2019-12-07 /pmc/articles/PMC6956034/ /pubmed/31817902 http://dx.doi.org/10.3390/microorganisms7120662 Text en © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Nerva, Luca
Pagliarani, Chiara
Pugliese, Massimo
Monchiero, Matteo
Gonthier, Solène
Gullino, Maria Lodovica
Gambino, Giorgio
Chitarra, Walter
Grapevine Phyllosphere Community Analysis in Response to Elicitor Application against Powdery Mildew
title Grapevine Phyllosphere Community Analysis in Response to Elicitor Application against Powdery Mildew
title_full Grapevine Phyllosphere Community Analysis in Response to Elicitor Application against Powdery Mildew
title_fullStr Grapevine Phyllosphere Community Analysis in Response to Elicitor Application against Powdery Mildew
title_full_unstemmed Grapevine Phyllosphere Community Analysis in Response to Elicitor Application against Powdery Mildew
title_short Grapevine Phyllosphere Community Analysis in Response to Elicitor Application against Powdery Mildew
title_sort grapevine phyllosphere community analysis in response to elicitor application against powdery mildew
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6956034/
https://www.ncbi.nlm.nih.gov/pubmed/31817902
http://dx.doi.org/10.3390/microorganisms7120662
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