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Preliminary Biochemical Description of Brain Oxidative Stress Status in Irritable Bowel Syndrome Contention-Stress Rat Model

Background and objectives: Oxidative stress and inflammation have been implicated in the etiology of irritable bowel syndrome (IBS), a common gastrointestinal functional disease. This study aimed to further characterize the contention-stress rat model by exploring a possible correlation between oxid...

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Autores principales: Balmus, Ioana-Miruna, Lefter, Radu, Ciobica, Alin, Cojocaru, Sabina, Guenne, Samson, Timofte, Daniel, Stanciu, Carol, Trifan, Anca, Hritcu, Luminita
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6956041/
https://www.ncbi.nlm.nih.gov/pubmed/31817740
http://dx.doi.org/10.3390/medicina55120776
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author Balmus, Ioana-Miruna
Lefter, Radu
Ciobica, Alin
Cojocaru, Sabina
Guenne, Samson
Timofte, Daniel
Stanciu, Carol
Trifan, Anca
Hritcu, Luminita
author_facet Balmus, Ioana-Miruna
Lefter, Radu
Ciobica, Alin
Cojocaru, Sabina
Guenne, Samson
Timofte, Daniel
Stanciu, Carol
Trifan, Anca
Hritcu, Luminita
author_sort Balmus, Ioana-Miruna
collection PubMed
description Background and objectives: Oxidative stress and inflammation have been implicated in the etiology of irritable bowel syndrome (IBS), a common gastrointestinal functional disease. This study aimed to further characterize the contention-stress rat model by exploring a possible correlation between oxidative stress markers measured in brain tissues with behavioral components of the aforementioned model. Thus, it is hereby proposed a possible IBS animal model relevant to pharmacological and complementary medicine studies. Materials and Methods: Wild-type male Wistar rats (n = 5/group) were chronically exposed to 6-hour/day contention, consisting of isolating the animals in small, vital space-granting plastic devices, for seven consecutive days. Following contention exposure, temporal lobes were extracted and subjected to biochemical analyses to assess oxidative stress-status parameters. Results: Our results show increased brain oxidative stress in contention-stress rat model: decreased superoxide dismutase and glutathione peroxidase activities and increased malondialdehyde production in the IBS group, as compared to the control group. Furthermore, the biochemical ratios which are used to evaluate the effectiveness of an antioxidant system on oxidative stress could be described in this model. Conclusions: The correlations between the behavioral patterns and biochemical oxidative stress features could suggest that this may be a complex model, which can successfully mimic IBS symptomatology further providing evidence of a strong connection between the digestive system, enteric nervous system, and the central nervous system.
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spelling pubmed-69560412020-01-23 Preliminary Biochemical Description of Brain Oxidative Stress Status in Irritable Bowel Syndrome Contention-Stress Rat Model Balmus, Ioana-Miruna Lefter, Radu Ciobica, Alin Cojocaru, Sabina Guenne, Samson Timofte, Daniel Stanciu, Carol Trifan, Anca Hritcu, Luminita Medicina (Kaunas) Article Background and objectives: Oxidative stress and inflammation have been implicated in the etiology of irritable bowel syndrome (IBS), a common gastrointestinal functional disease. This study aimed to further characterize the contention-stress rat model by exploring a possible correlation between oxidative stress markers measured in brain tissues with behavioral components of the aforementioned model. Thus, it is hereby proposed a possible IBS animal model relevant to pharmacological and complementary medicine studies. Materials and Methods: Wild-type male Wistar rats (n = 5/group) were chronically exposed to 6-hour/day contention, consisting of isolating the animals in small, vital space-granting plastic devices, for seven consecutive days. Following contention exposure, temporal lobes were extracted and subjected to biochemical analyses to assess oxidative stress-status parameters. Results: Our results show increased brain oxidative stress in contention-stress rat model: decreased superoxide dismutase and glutathione peroxidase activities and increased malondialdehyde production in the IBS group, as compared to the control group. Furthermore, the biochemical ratios which are used to evaluate the effectiveness of an antioxidant system on oxidative stress could be described in this model. Conclusions: The correlations between the behavioral patterns and biochemical oxidative stress features could suggest that this may be a complex model, which can successfully mimic IBS symptomatology further providing evidence of a strong connection between the digestive system, enteric nervous system, and the central nervous system. MDPI 2019-12-06 /pmc/articles/PMC6956041/ /pubmed/31817740 http://dx.doi.org/10.3390/medicina55120776 Text en © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Balmus, Ioana-Miruna
Lefter, Radu
Ciobica, Alin
Cojocaru, Sabina
Guenne, Samson
Timofte, Daniel
Stanciu, Carol
Trifan, Anca
Hritcu, Luminita
Preliminary Biochemical Description of Brain Oxidative Stress Status in Irritable Bowel Syndrome Contention-Stress Rat Model
title Preliminary Biochemical Description of Brain Oxidative Stress Status in Irritable Bowel Syndrome Contention-Stress Rat Model
title_full Preliminary Biochemical Description of Brain Oxidative Stress Status in Irritable Bowel Syndrome Contention-Stress Rat Model
title_fullStr Preliminary Biochemical Description of Brain Oxidative Stress Status in Irritable Bowel Syndrome Contention-Stress Rat Model
title_full_unstemmed Preliminary Biochemical Description of Brain Oxidative Stress Status in Irritable Bowel Syndrome Contention-Stress Rat Model
title_short Preliminary Biochemical Description of Brain Oxidative Stress Status in Irritable Bowel Syndrome Contention-Stress Rat Model
title_sort preliminary biochemical description of brain oxidative stress status in irritable bowel syndrome contention-stress rat model
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6956041/
https://www.ncbi.nlm.nih.gov/pubmed/31817740
http://dx.doi.org/10.3390/medicina55120776
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