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Chlamydia trachomatis Whole-Proteome Microarray Analysis of The Netherlands Chlamydia Cohort Study

Chlamydia trachomatis (Ct) whole-proteome microarrays were utilized to identify antibody patterns associated with infection; pelvic inflammatory disease (PID), tubal factor infertility, chronic pelvic pain (CPP) and ectopic pregnancy in a subsample of the Netherlands Chlamydia cohort study. Serum po...

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Autores principales: Hufnagel, Katrin, Hoenderboom, Bernice, Harmel, Christoph, Rohland, Juliane K., van Benthem, Birgit H.B., Morré, Servaas A., Waterboer, Tim
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6956083/
https://www.ncbi.nlm.nih.gov/pubmed/31888186
http://dx.doi.org/10.3390/microorganisms7120703
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author Hufnagel, Katrin
Hoenderboom, Bernice
Harmel, Christoph
Rohland, Juliane K.
van Benthem, Birgit H.B.
Morré, Servaas A.
Waterboer, Tim
author_facet Hufnagel, Katrin
Hoenderboom, Bernice
Harmel, Christoph
Rohland, Juliane K.
van Benthem, Birgit H.B.
Morré, Servaas A.
Waterboer, Tim
author_sort Hufnagel, Katrin
collection PubMed
description Chlamydia trachomatis (Ct) whole-proteome microarrays were utilized to identify antibody patterns associated with infection; pelvic inflammatory disease (PID), tubal factor infertility, chronic pelvic pain (CPP) and ectopic pregnancy in a subsample of the Netherlands Chlamydia cohort study. Serum pools were analyzed on whole-proteome arrays. The 121 most reactive antigens identified during whole-proteome arrays were selected for further analysis with minimized microarrays that allowed for single sera analysis. From the 232 single sera; 145 (62.5%) serum samples were reactive for at least one antigen. To discriminate between positive and negative serum samples; we created a panel of in total 18 antigens which identified 96% of all microarray positive samples. Antigens CT_858; CT_813 and CT_142 were most reactive. Comparison of antibody reactivity’s among women with and without Ct related sequelae revealed that the reactivity of CT_813 and CT_142 was less common among women with PID compared to women without (29.0% versus 58.6%, p = 0.005 and 25.8% versus 50.6%, p = 0.017 respectively). CT_858 was less common among CPP cases compared to controls (33.3% versus 58.6; p = 0.028). Using a whole-proteome array to select antigens for minimized arrays allows for the identification of novel informative antigens as general infection markers or disease associated antigens
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spelling pubmed-69560832020-01-23 Chlamydia trachomatis Whole-Proteome Microarray Analysis of The Netherlands Chlamydia Cohort Study Hufnagel, Katrin Hoenderboom, Bernice Harmel, Christoph Rohland, Juliane K. van Benthem, Birgit H.B. Morré, Servaas A. Waterboer, Tim Microorganisms Article Chlamydia trachomatis (Ct) whole-proteome microarrays were utilized to identify antibody patterns associated with infection; pelvic inflammatory disease (PID), tubal factor infertility, chronic pelvic pain (CPP) and ectopic pregnancy in a subsample of the Netherlands Chlamydia cohort study. Serum pools were analyzed on whole-proteome arrays. The 121 most reactive antigens identified during whole-proteome arrays were selected for further analysis with minimized microarrays that allowed for single sera analysis. From the 232 single sera; 145 (62.5%) serum samples were reactive for at least one antigen. To discriminate between positive and negative serum samples; we created a panel of in total 18 antigens which identified 96% of all microarray positive samples. Antigens CT_858; CT_813 and CT_142 were most reactive. Comparison of antibody reactivity’s among women with and without Ct related sequelae revealed that the reactivity of CT_813 and CT_142 was less common among women with PID compared to women without (29.0% versus 58.6%, p = 0.005 and 25.8% versus 50.6%, p = 0.017 respectively). CT_858 was less common among CPP cases compared to controls (33.3% versus 58.6; p = 0.028). Using a whole-proteome array to select antigens for minimized arrays allows for the identification of novel informative antigens as general infection markers or disease associated antigens MDPI 2019-12-16 /pmc/articles/PMC6956083/ /pubmed/31888186 http://dx.doi.org/10.3390/microorganisms7120703 Text en © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Hufnagel, Katrin
Hoenderboom, Bernice
Harmel, Christoph
Rohland, Juliane K.
van Benthem, Birgit H.B.
Morré, Servaas A.
Waterboer, Tim
Chlamydia trachomatis Whole-Proteome Microarray Analysis of The Netherlands Chlamydia Cohort Study
title Chlamydia trachomatis Whole-Proteome Microarray Analysis of The Netherlands Chlamydia Cohort Study
title_full Chlamydia trachomatis Whole-Proteome Microarray Analysis of The Netherlands Chlamydia Cohort Study
title_fullStr Chlamydia trachomatis Whole-Proteome Microarray Analysis of The Netherlands Chlamydia Cohort Study
title_full_unstemmed Chlamydia trachomatis Whole-Proteome Microarray Analysis of The Netherlands Chlamydia Cohort Study
title_short Chlamydia trachomatis Whole-Proteome Microarray Analysis of The Netherlands Chlamydia Cohort Study
title_sort chlamydia trachomatis whole-proteome microarray analysis of the netherlands chlamydia cohort study
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6956083/
https://www.ncbi.nlm.nih.gov/pubmed/31888186
http://dx.doi.org/10.3390/microorganisms7120703
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