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Nutrient Excess Triggers the Expression of the Penicillium chrysogenum Antifungal Protein PAFB
Nutrient limitation and nonfavorable growth conditions have been suggested to be major triggers for the expression of small, cysteine-rich antimicrobial proteins (AMPs) of fungal origin, e.g., the Penicillium chrysogenum antifungal protein (PAF), the Aspergillus giganteus antifungal protein (AFP), t...
Autores principales: | , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6956099/ https://www.ncbi.nlm.nih.gov/pubmed/31817241 http://dx.doi.org/10.3390/microorganisms7120654 |
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author | Huber, Anna Lerchster, Hannah Marx, Florentine |
author_facet | Huber, Anna Lerchster, Hannah Marx, Florentine |
author_sort | Huber, Anna |
collection | PubMed |
description | Nutrient limitation and nonfavorable growth conditions have been suggested to be major triggers for the expression of small, cysteine-rich antimicrobial proteins (AMPs) of fungal origin, e.g., the Penicillium chrysogenum antifungal protein (PAF), the Aspergillus giganteus antifungal protein (AFP), the Aspergillus niger antifungal protein (AnAFP). Therefore, these AMPs have been considered to be fungal secondary metabolite products. In contrast, the present study revealed that the expression of the PAF-related AMP P. chrysogenum antifungal protein B (PAFB) is strongly induced under nutrient excess during the logarithmic growth phase, whereas PAFB remained under the detection level in the supernatant of cultures grown under nutrient limitation. The efficiency of the pafB-promoter to induce PAFB expression was compared with that of two P. chrysogenum promoters that are well established for recombinant protein production: the paf-promoter and the xylose-inducible promoter of the xylanase gene, xylP. The inducibility of the pafB-promoter was superior to that of the xylP-promoter yielding comparable PAFB amounts as under the regulation of the paf-promoter. We conclude that (i) differences in the expression regulation of AMPs suggest distinct functional roles in the producer beyond their antifungal activity; and (ii) the pafB-promoter is a promising tool for recombinant protein production in P. chrysogenum, as it guarantees strong gene expression with the advantage of inducibility. |
format | Online Article Text |
id | pubmed-6956099 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-69560992020-01-23 Nutrient Excess Triggers the Expression of the Penicillium chrysogenum Antifungal Protein PAFB Huber, Anna Lerchster, Hannah Marx, Florentine Microorganisms Article Nutrient limitation and nonfavorable growth conditions have been suggested to be major triggers for the expression of small, cysteine-rich antimicrobial proteins (AMPs) of fungal origin, e.g., the Penicillium chrysogenum antifungal protein (PAF), the Aspergillus giganteus antifungal protein (AFP), the Aspergillus niger antifungal protein (AnAFP). Therefore, these AMPs have been considered to be fungal secondary metabolite products. In contrast, the present study revealed that the expression of the PAF-related AMP P. chrysogenum antifungal protein B (PAFB) is strongly induced under nutrient excess during the logarithmic growth phase, whereas PAFB remained under the detection level in the supernatant of cultures grown under nutrient limitation. The efficiency of the pafB-promoter to induce PAFB expression was compared with that of two P. chrysogenum promoters that are well established for recombinant protein production: the paf-promoter and the xylose-inducible promoter of the xylanase gene, xylP. The inducibility of the pafB-promoter was superior to that of the xylP-promoter yielding comparable PAFB amounts as under the regulation of the paf-promoter. We conclude that (i) differences in the expression regulation of AMPs suggest distinct functional roles in the producer beyond their antifungal activity; and (ii) the pafB-promoter is a promising tool for recombinant protein production in P. chrysogenum, as it guarantees strong gene expression with the advantage of inducibility. MDPI 2019-12-04 /pmc/articles/PMC6956099/ /pubmed/31817241 http://dx.doi.org/10.3390/microorganisms7120654 Text en © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Huber, Anna Lerchster, Hannah Marx, Florentine Nutrient Excess Triggers the Expression of the Penicillium chrysogenum Antifungal Protein PAFB |
title | Nutrient Excess Triggers the Expression of the Penicillium chrysogenum Antifungal Protein PAFB |
title_full | Nutrient Excess Triggers the Expression of the Penicillium chrysogenum Antifungal Protein PAFB |
title_fullStr | Nutrient Excess Triggers the Expression of the Penicillium chrysogenum Antifungal Protein PAFB |
title_full_unstemmed | Nutrient Excess Triggers the Expression of the Penicillium chrysogenum Antifungal Protein PAFB |
title_short | Nutrient Excess Triggers the Expression of the Penicillium chrysogenum Antifungal Protein PAFB |
title_sort | nutrient excess triggers the expression of the penicillium chrysogenum antifungal protein pafb |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6956099/ https://www.ncbi.nlm.nih.gov/pubmed/31817241 http://dx.doi.org/10.3390/microorganisms7120654 |
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