Cargando…

Nutrient Excess Triggers the Expression of the Penicillium chrysogenum Antifungal Protein PAFB

Nutrient limitation and nonfavorable growth conditions have been suggested to be major triggers for the expression of small, cysteine-rich antimicrobial proteins (AMPs) of fungal origin, e.g., the Penicillium chrysogenum antifungal protein (PAF), the Aspergillus giganteus antifungal protein (AFP), t...

Descripción completa

Detalles Bibliográficos
Autores principales: Huber, Anna, Lerchster, Hannah, Marx, Florentine
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6956099/
https://www.ncbi.nlm.nih.gov/pubmed/31817241
http://dx.doi.org/10.3390/microorganisms7120654
_version_ 1783487082666131456
author Huber, Anna
Lerchster, Hannah
Marx, Florentine
author_facet Huber, Anna
Lerchster, Hannah
Marx, Florentine
author_sort Huber, Anna
collection PubMed
description Nutrient limitation and nonfavorable growth conditions have been suggested to be major triggers for the expression of small, cysteine-rich antimicrobial proteins (AMPs) of fungal origin, e.g., the Penicillium chrysogenum antifungal protein (PAF), the Aspergillus giganteus antifungal protein (AFP), the Aspergillus niger antifungal protein (AnAFP). Therefore, these AMPs have been considered to be fungal secondary metabolite products. In contrast, the present study revealed that the expression of the PAF-related AMP P. chrysogenum antifungal protein B (PAFB) is strongly induced under nutrient excess during the logarithmic growth phase, whereas PAFB remained under the detection level in the supernatant of cultures grown under nutrient limitation. The efficiency of the pafB-promoter to induce PAFB expression was compared with that of two P. chrysogenum promoters that are well established for recombinant protein production: the paf-promoter and the xylose-inducible promoter of the xylanase gene, xylP. The inducibility of the pafB-promoter was superior to that of the xylP-promoter yielding comparable PAFB amounts as under the regulation of the paf-promoter. We conclude that (i) differences in the expression regulation of AMPs suggest distinct functional roles in the producer beyond their antifungal activity; and (ii) the pafB-promoter is a promising tool for recombinant protein production in P. chrysogenum, as it guarantees strong gene expression with the advantage of inducibility.
format Online
Article
Text
id pubmed-6956099
institution National Center for Biotechnology Information
language English
publishDate 2019
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-69560992020-01-23 Nutrient Excess Triggers the Expression of the Penicillium chrysogenum Antifungal Protein PAFB Huber, Anna Lerchster, Hannah Marx, Florentine Microorganisms Article Nutrient limitation and nonfavorable growth conditions have been suggested to be major triggers for the expression of small, cysteine-rich antimicrobial proteins (AMPs) of fungal origin, e.g., the Penicillium chrysogenum antifungal protein (PAF), the Aspergillus giganteus antifungal protein (AFP), the Aspergillus niger antifungal protein (AnAFP). Therefore, these AMPs have been considered to be fungal secondary metabolite products. In contrast, the present study revealed that the expression of the PAF-related AMP P. chrysogenum antifungal protein B (PAFB) is strongly induced under nutrient excess during the logarithmic growth phase, whereas PAFB remained under the detection level in the supernatant of cultures grown under nutrient limitation. The efficiency of the pafB-promoter to induce PAFB expression was compared with that of two P. chrysogenum promoters that are well established for recombinant protein production: the paf-promoter and the xylose-inducible promoter of the xylanase gene, xylP. The inducibility of the pafB-promoter was superior to that of the xylP-promoter yielding comparable PAFB amounts as under the regulation of the paf-promoter. We conclude that (i) differences in the expression regulation of AMPs suggest distinct functional roles in the producer beyond their antifungal activity; and (ii) the pafB-promoter is a promising tool for recombinant protein production in P. chrysogenum, as it guarantees strong gene expression with the advantage of inducibility. MDPI 2019-12-04 /pmc/articles/PMC6956099/ /pubmed/31817241 http://dx.doi.org/10.3390/microorganisms7120654 Text en © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Huber, Anna
Lerchster, Hannah
Marx, Florentine
Nutrient Excess Triggers the Expression of the Penicillium chrysogenum Antifungal Protein PAFB
title Nutrient Excess Triggers the Expression of the Penicillium chrysogenum Antifungal Protein PAFB
title_full Nutrient Excess Triggers the Expression of the Penicillium chrysogenum Antifungal Protein PAFB
title_fullStr Nutrient Excess Triggers the Expression of the Penicillium chrysogenum Antifungal Protein PAFB
title_full_unstemmed Nutrient Excess Triggers the Expression of the Penicillium chrysogenum Antifungal Protein PAFB
title_short Nutrient Excess Triggers the Expression of the Penicillium chrysogenum Antifungal Protein PAFB
title_sort nutrient excess triggers the expression of the penicillium chrysogenum antifungal protein pafb
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6956099/
https://www.ncbi.nlm.nih.gov/pubmed/31817241
http://dx.doi.org/10.3390/microorganisms7120654
work_keys_str_mv AT huberanna nutrientexcesstriggerstheexpressionofthepenicilliumchrysogenumantifungalproteinpafb
AT lerchsterhannah nutrientexcesstriggerstheexpressionofthepenicilliumchrysogenumantifungalproteinpafb
AT marxflorentine nutrientexcesstriggerstheexpressionofthepenicilliumchrysogenumantifungalproteinpafb