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Blastocystis Colonization Is Associated with Increased Diversity and Altered Gut Bacterial Communities in Healthy Malian Children

Blastocystis is the most common protozoan colonizing the gut of vertebrates. It modulates the human digestive microbiota in the absence of inflammation and gastrointestinal disease. Although it has been associated with human diseases, including inflammatory bowel disease, its pathogenicity remains c...

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Autores principales: Kodio, Aly, Coulibaly, Drissa, Koné, Abdoulaye Kassoum, Konaté, Salimata, Doumbo, Safiatou, Guindo, Abdoulaye, Bittar, Fadi, Gouriet, Frédérique, Raoult, Didier, Thera, Mahamadou Aly, Ranque, Stéphane
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6956266/
https://www.ncbi.nlm.nih.gov/pubmed/31817168
http://dx.doi.org/10.3390/microorganisms7120649
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author Kodio, Aly
Coulibaly, Drissa
Koné, Abdoulaye Kassoum
Konaté, Salimata
Doumbo, Safiatou
Guindo, Abdoulaye
Bittar, Fadi
Gouriet, Frédérique
Raoult, Didier
Thera, Mahamadou Aly
Ranque, Stéphane
author_facet Kodio, Aly
Coulibaly, Drissa
Koné, Abdoulaye Kassoum
Konaté, Salimata
Doumbo, Safiatou
Guindo, Abdoulaye
Bittar, Fadi
Gouriet, Frédérique
Raoult, Didier
Thera, Mahamadou Aly
Ranque, Stéphane
author_sort Kodio, Aly
collection PubMed
description Blastocystis is the most common protozoan colonizing the gut of vertebrates. It modulates the human digestive microbiota in the absence of inflammation and gastrointestinal disease. Although it has been associated with human diseases, including inflammatory bowel disease, its pathogenicity remains controversial. This study aimed to assess the influence of Blastocystis on the gut bacterial communities in healthy children. We conducted a cross-sectional study on 147 Blastocystis-colonized and 149 Blastocystis-noncolonized Malian children, with Blastocystis colonization assessed by real-time PCR and gut microbial communities characterized via 16S rRNA gene (Illumina MiSeq) sequencing and bioinformatics analysis. The gut microbiota diversity was higher in Blastocystis-colonized compared to Blastocystis-noncolonized children. The phyla Firmicutes, Elusimicrobia, Lentisphaerae, and Euryarchaeota were higher in Blastocystis-colonized children, whereas Actinobacteria, Proteobacteria, unassigned bacteria, and Deinococcus–Thermus were higher in Blastocystis-noncolonized children. Moreover, Faecalibacterium prausnitzii (family Ruminococcaceae) and Roseburia sp. (family Lachnospiraceae) abundance was higher in Blastocystis-colonized children. We conclude that Blastocystis colonization is significantly associated with a higher diversity of the gut bacterial communities in healthy children, while it is not associated with the presence of potentially pathogenic bacteria in the human gut.
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spelling pubmed-69562662020-01-23 Blastocystis Colonization Is Associated with Increased Diversity and Altered Gut Bacterial Communities in Healthy Malian Children Kodio, Aly Coulibaly, Drissa Koné, Abdoulaye Kassoum Konaté, Salimata Doumbo, Safiatou Guindo, Abdoulaye Bittar, Fadi Gouriet, Frédérique Raoult, Didier Thera, Mahamadou Aly Ranque, Stéphane Microorganisms Article Blastocystis is the most common protozoan colonizing the gut of vertebrates. It modulates the human digestive microbiota in the absence of inflammation and gastrointestinal disease. Although it has been associated with human diseases, including inflammatory bowel disease, its pathogenicity remains controversial. This study aimed to assess the influence of Blastocystis on the gut bacterial communities in healthy children. We conducted a cross-sectional study on 147 Blastocystis-colonized and 149 Blastocystis-noncolonized Malian children, with Blastocystis colonization assessed by real-time PCR and gut microbial communities characterized via 16S rRNA gene (Illumina MiSeq) sequencing and bioinformatics analysis. The gut microbiota diversity was higher in Blastocystis-colonized compared to Blastocystis-noncolonized children. The phyla Firmicutes, Elusimicrobia, Lentisphaerae, and Euryarchaeota were higher in Blastocystis-colonized children, whereas Actinobacteria, Proteobacteria, unassigned bacteria, and Deinococcus–Thermus were higher in Blastocystis-noncolonized children. Moreover, Faecalibacterium prausnitzii (family Ruminococcaceae) and Roseburia sp. (family Lachnospiraceae) abundance was higher in Blastocystis-colonized children. We conclude that Blastocystis colonization is significantly associated with a higher diversity of the gut bacterial communities in healthy children, while it is not associated with the presence of potentially pathogenic bacteria in the human gut. MDPI 2019-12-04 /pmc/articles/PMC6956266/ /pubmed/31817168 http://dx.doi.org/10.3390/microorganisms7120649 Text en © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Kodio, Aly
Coulibaly, Drissa
Koné, Abdoulaye Kassoum
Konaté, Salimata
Doumbo, Safiatou
Guindo, Abdoulaye
Bittar, Fadi
Gouriet, Frédérique
Raoult, Didier
Thera, Mahamadou Aly
Ranque, Stéphane
Blastocystis Colonization Is Associated with Increased Diversity and Altered Gut Bacterial Communities in Healthy Malian Children
title Blastocystis Colonization Is Associated with Increased Diversity and Altered Gut Bacterial Communities in Healthy Malian Children
title_full Blastocystis Colonization Is Associated with Increased Diversity and Altered Gut Bacterial Communities in Healthy Malian Children
title_fullStr Blastocystis Colonization Is Associated with Increased Diversity and Altered Gut Bacterial Communities in Healthy Malian Children
title_full_unstemmed Blastocystis Colonization Is Associated with Increased Diversity and Altered Gut Bacterial Communities in Healthy Malian Children
title_short Blastocystis Colonization Is Associated with Increased Diversity and Altered Gut Bacterial Communities in Healthy Malian Children
title_sort blastocystis colonization is associated with increased diversity and altered gut bacterial communities in healthy malian children
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6956266/
https://www.ncbi.nlm.nih.gov/pubmed/31817168
http://dx.doi.org/10.3390/microorganisms7120649
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