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Effects of Pulsed Electric Field on the Cell Wall and Infection Activity of Rhizoctonia solani
This paper adopts the Design-Expert software to design an orthogonal experiment with a pulse voltage amplitude of 30 kV, processing time of three minutes, and a pulse width of 45 μs as the center points, in order to study the effects of the pulsed electric field on the cell wall and infection activi...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6956288/ https://www.ncbi.nlm.nih.gov/pubmed/31561587 http://dx.doi.org/10.3390/biology8040073 |
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author | Feng, Xuebin He, Peijun Pan, Chaoya Xu, Jin Xue, Baoshan Yin, Wenqing Qian, Yan |
author_facet | Feng, Xuebin He, Peijun Pan, Chaoya Xu, Jin Xue, Baoshan Yin, Wenqing Qian, Yan |
author_sort | Feng, Xuebin |
collection | PubMed |
description | This paper adopts the Design-Expert software to design an orthogonal experiment with a pulse voltage amplitude of 30 kV, processing time of three minutes, and a pulse width of 45 μs as the center points, in order to study the effects of the pulsed electric field on the cell wall and infection activity of Rhizoctonia solani. High-voltage pulse power was used to treat the bacteria solution with the pulsed electric field. Untreated Rhizoctonia solani were used as the control group. Transmission electron microscope images were used to analyze the cell wall damage. ANOVA was performed on the experimental results and the fitting degree of the model was good (F>>1). Response surface analysis was used to optimize the parameters based on chitin content and polygalacturonase activity. The optimal treatment conditions were obtained as a pulse voltage amplitude of 25 kV, processing time of 2.54 min, and a pulse width of 34.35 μs. On this basis, experiments were designed to verify the optimized conditions. The results demonstrated that, under the optimal processing conditions, the damage index of the cell wall of Rhizoctonia solani was 9.59% lower in chitin content and 83.05% lower in polygalacturonase activity compared with those of the control group. All indexes were significantly different (P < 0.001), which is consistent with the parameter optimization results. The results provide a theoretical basis for the pulsed electric field assisted sterilization and reference for the design of plant protection machinery in the latter stage. |
format | Online Article Text |
id | pubmed-6956288 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-69562882020-01-23 Effects of Pulsed Electric Field on the Cell Wall and Infection Activity of Rhizoctonia solani Feng, Xuebin He, Peijun Pan, Chaoya Xu, Jin Xue, Baoshan Yin, Wenqing Qian, Yan Biology (Basel) Article This paper adopts the Design-Expert software to design an orthogonal experiment with a pulse voltage amplitude of 30 kV, processing time of three minutes, and a pulse width of 45 μs as the center points, in order to study the effects of the pulsed electric field on the cell wall and infection activity of Rhizoctonia solani. High-voltage pulse power was used to treat the bacteria solution with the pulsed electric field. Untreated Rhizoctonia solani were used as the control group. Transmission electron microscope images were used to analyze the cell wall damage. ANOVA was performed on the experimental results and the fitting degree of the model was good (F>>1). Response surface analysis was used to optimize the parameters based on chitin content and polygalacturonase activity. The optimal treatment conditions were obtained as a pulse voltage amplitude of 25 kV, processing time of 2.54 min, and a pulse width of 34.35 μs. On this basis, experiments were designed to verify the optimized conditions. The results demonstrated that, under the optimal processing conditions, the damage index of the cell wall of Rhizoctonia solani was 9.59% lower in chitin content and 83.05% lower in polygalacturonase activity compared with those of the control group. All indexes were significantly different (P < 0.001), which is consistent with the parameter optimization results. The results provide a theoretical basis for the pulsed electric field assisted sterilization and reference for the design of plant protection machinery in the latter stage. MDPI 2019-09-26 /pmc/articles/PMC6956288/ /pubmed/31561587 http://dx.doi.org/10.3390/biology8040073 Text en © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Feng, Xuebin He, Peijun Pan, Chaoya Xu, Jin Xue, Baoshan Yin, Wenqing Qian, Yan Effects of Pulsed Electric Field on the Cell Wall and Infection Activity of Rhizoctonia solani |
title | Effects of Pulsed Electric Field on the Cell Wall and Infection Activity of Rhizoctonia solani |
title_full | Effects of Pulsed Electric Field on the Cell Wall and Infection Activity of Rhizoctonia solani |
title_fullStr | Effects of Pulsed Electric Field on the Cell Wall and Infection Activity of Rhizoctonia solani |
title_full_unstemmed | Effects of Pulsed Electric Field on the Cell Wall and Infection Activity of Rhizoctonia solani |
title_short | Effects of Pulsed Electric Field on the Cell Wall and Infection Activity of Rhizoctonia solani |
title_sort | effects of pulsed electric field on the cell wall and infection activity of rhizoctonia solani |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6956288/ https://www.ncbi.nlm.nih.gov/pubmed/31561587 http://dx.doi.org/10.3390/biology8040073 |
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