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Effects of Pulsed Electric Field on the Cell Wall and Infection Activity of Rhizoctonia solani

This paper adopts the Design-Expert software to design an orthogonal experiment with a pulse voltage amplitude of 30 kV, processing time of three minutes, and a pulse width of 45 μs as the center points, in order to study the effects of the pulsed electric field on the cell wall and infection activi...

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Detalles Bibliográficos
Autores principales: Feng, Xuebin, He, Peijun, Pan, Chaoya, Xu, Jin, Xue, Baoshan, Yin, Wenqing, Qian, Yan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6956288/
https://www.ncbi.nlm.nih.gov/pubmed/31561587
http://dx.doi.org/10.3390/biology8040073
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author Feng, Xuebin
He, Peijun
Pan, Chaoya
Xu, Jin
Xue, Baoshan
Yin, Wenqing
Qian, Yan
author_facet Feng, Xuebin
He, Peijun
Pan, Chaoya
Xu, Jin
Xue, Baoshan
Yin, Wenqing
Qian, Yan
author_sort Feng, Xuebin
collection PubMed
description This paper adopts the Design-Expert software to design an orthogonal experiment with a pulse voltage amplitude of 30 kV, processing time of three minutes, and a pulse width of 45 μs as the center points, in order to study the effects of the pulsed electric field on the cell wall and infection activity of Rhizoctonia solani. High-voltage pulse power was used to treat the bacteria solution with the pulsed electric field. Untreated Rhizoctonia solani were used as the control group. Transmission electron microscope images were used to analyze the cell wall damage. ANOVA was performed on the experimental results and the fitting degree of the model was good (F>>1). Response surface analysis was used to optimize the parameters based on chitin content and polygalacturonase activity. The optimal treatment conditions were obtained as a pulse voltage amplitude of 25 kV, processing time of 2.54 min, and a pulse width of 34.35 μs. On this basis, experiments were designed to verify the optimized conditions. The results demonstrated that, under the optimal processing conditions, the damage index of the cell wall of Rhizoctonia solani was 9.59% lower in chitin content and 83.05% lower in polygalacturonase activity compared with those of the control group. All indexes were significantly different (P < 0.001), which is consistent with the parameter optimization results. The results provide a theoretical basis for the pulsed electric field assisted sterilization and reference for the design of plant protection machinery in the latter stage.
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spelling pubmed-69562882020-01-23 Effects of Pulsed Electric Field on the Cell Wall and Infection Activity of Rhizoctonia solani Feng, Xuebin He, Peijun Pan, Chaoya Xu, Jin Xue, Baoshan Yin, Wenqing Qian, Yan Biology (Basel) Article This paper adopts the Design-Expert software to design an orthogonal experiment with a pulse voltage amplitude of 30 kV, processing time of three minutes, and a pulse width of 45 μs as the center points, in order to study the effects of the pulsed electric field on the cell wall and infection activity of Rhizoctonia solani. High-voltage pulse power was used to treat the bacteria solution with the pulsed electric field. Untreated Rhizoctonia solani were used as the control group. Transmission electron microscope images were used to analyze the cell wall damage. ANOVA was performed on the experimental results and the fitting degree of the model was good (F>>1). Response surface analysis was used to optimize the parameters based on chitin content and polygalacturonase activity. The optimal treatment conditions were obtained as a pulse voltage amplitude of 25 kV, processing time of 2.54 min, and a pulse width of 34.35 μs. On this basis, experiments were designed to verify the optimized conditions. The results demonstrated that, under the optimal processing conditions, the damage index of the cell wall of Rhizoctonia solani was 9.59% lower in chitin content and 83.05% lower in polygalacturonase activity compared with those of the control group. All indexes were significantly different (P < 0.001), which is consistent with the parameter optimization results. The results provide a theoretical basis for the pulsed electric field assisted sterilization and reference for the design of plant protection machinery in the latter stage. MDPI 2019-09-26 /pmc/articles/PMC6956288/ /pubmed/31561587 http://dx.doi.org/10.3390/biology8040073 Text en © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Feng, Xuebin
He, Peijun
Pan, Chaoya
Xu, Jin
Xue, Baoshan
Yin, Wenqing
Qian, Yan
Effects of Pulsed Electric Field on the Cell Wall and Infection Activity of Rhizoctonia solani
title Effects of Pulsed Electric Field on the Cell Wall and Infection Activity of Rhizoctonia solani
title_full Effects of Pulsed Electric Field on the Cell Wall and Infection Activity of Rhizoctonia solani
title_fullStr Effects of Pulsed Electric Field on the Cell Wall and Infection Activity of Rhizoctonia solani
title_full_unstemmed Effects of Pulsed Electric Field on the Cell Wall and Infection Activity of Rhizoctonia solani
title_short Effects of Pulsed Electric Field on the Cell Wall and Infection Activity of Rhizoctonia solani
title_sort effects of pulsed electric field on the cell wall and infection activity of rhizoctonia solani
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6956288/
https://www.ncbi.nlm.nih.gov/pubmed/31561587
http://dx.doi.org/10.3390/biology8040073
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