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Indexes of Angiogenic Activation in Myocardial Samples of Patients with Advanced Chronic Heart Failure

Background and objectives: Ischemic and idiopathic heart failure are characterized by reactive cardiac fibrosis and impaired vasculogenesis involving pro-angiogenic factors such as angiogenin, angiopoietin-1 (Ang-1), and angiopoietin-2 (Ang-2), as demonstrated in experimental models of heart failure...

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Autores principales: Komici, Klara, Gnemmi, Isabella, Sangiorgi, Claudia, Ricciardolo, Fabio Luigi Massimo, Rinaldi, Mauro, Di Stefano, Antonino, Eleuteri, Ermanno
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6956299/
https://www.ncbi.nlm.nih.gov/pubmed/31795484
http://dx.doi.org/10.3390/medicina55120766
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author Komici, Klara
Gnemmi, Isabella
Sangiorgi, Claudia
Ricciardolo, Fabio Luigi Massimo
Rinaldi, Mauro
Di Stefano, Antonino
Eleuteri, Ermanno
author_facet Komici, Klara
Gnemmi, Isabella
Sangiorgi, Claudia
Ricciardolo, Fabio Luigi Massimo
Rinaldi, Mauro
Di Stefano, Antonino
Eleuteri, Ermanno
author_sort Komici, Klara
collection PubMed
description Background and objectives: Ischemic and idiopathic heart failure are characterized by reactive cardiac fibrosis and impaired vasculogenesis involving pro-angiogenic factors such as angiogenin, angiopoietin-1 (Ang-1), and angiopoietin-2 (Ang-2), as demonstrated in experimental models of heart failure. However, differences in the molecular pathways between these cardiomyopathies are still unclear. In this short communication, we evaluate and compare the expression of pro-angiogenic molecules in the heart tissue of patients with advanced chronic heart failure (CHF) of ischemic vs. nonischemic etiology. Materials and Methods: We obtained heart tissue at transplantation from left ventricular walls of 16 explanted native hearts affected by either ischemic (ICM) or nonischemic dilated cardiomyopathy (NIDCM). Tissue samples were examined using immunohistochemistry for angiogenic molecules. Results: We found immunopositivity (I-pos) for angiopoietin-1 mainly in the cardiomyocytes, while we observed I-pos for Ang-2 and Tie-2 receptor mainly in endothelial cells. Expression of Procollagen-I (PICP), angiogenin, Ang-1, and Tie-2 receptor was similar in ICM and NIDCM. In contrast, endothelial immunopositivity for Ang-2 was higher in ICM samples than NIDCM (p = 0.03). Conclusions: In our series of CHF heart samples, distribution of Ang-1 and angiogenin was higher in cardiomyocytes while that of Ang-2 was higher in endothelial cells; moreover, Ang-2 expression was higher in ICS than NIDCM. Despite the small series examined, these findings suggest different patterns of angiogenic stimulation in ICM and NIDCM, or at least a more altered endothelial integrity in ICD. Our data may contribute to a better understanding of the angiogenesis signaling pathways in CHF. Further studies should investigate differences in the biochemical processes leading to heart failure.
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spelling pubmed-69562992020-01-23 Indexes of Angiogenic Activation in Myocardial Samples of Patients with Advanced Chronic Heart Failure Komici, Klara Gnemmi, Isabella Sangiorgi, Claudia Ricciardolo, Fabio Luigi Massimo Rinaldi, Mauro Di Stefano, Antonino Eleuteri, Ermanno Medicina (Kaunas) Communication Background and objectives: Ischemic and idiopathic heart failure are characterized by reactive cardiac fibrosis and impaired vasculogenesis involving pro-angiogenic factors such as angiogenin, angiopoietin-1 (Ang-1), and angiopoietin-2 (Ang-2), as demonstrated in experimental models of heart failure. However, differences in the molecular pathways between these cardiomyopathies are still unclear. In this short communication, we evaluate and compare the expression of pro-angiogenic molecules in the heart tissue of patients with advanced chronic heart failure (CHF) of ischemic vs. nonischemic etiology. Materials and Methods: We obtained heart tissue at transplantation from left ventricular walls of 16 explanted native hearts affected by either ischemic (ICM) or nonischemic dilated cardiomyopathy (NIDCM). Tissue samples were examined using immunohistochemistry for angiogenic molecules. Results: We found immunopositivity (I-pos) for angiopoietin-1 mainly in the cardiomyocytes, while we observed I-pos for Ang-2 and Tie-2 receptor mainly in endothelial cells. Expression of Procollagen-I (PICP), angiogenin, Ang-1, and Tie-2 receptor was similar in ICM and NIDCM. In contrast, endothelial immunopositivity for Ang-2 was higher in ICM samples than NIDCM (p = 0.03). Conclusions: In our series of CHF heart samples, distribution of Ang-1 and angiogenin was higher in cardiomyocytes while that of Ang-2 was higher in endothelial cells; moreover, Ang-2 expression was higher in ICS than NIDCM. Despite the small series examined, these findings suggest different patterns of angiogenic stimulation in ICM and NIDCM, or at least a more altered endothelial integrity in ICD. Our data may contribute to a better understanding of the angiogenesis signaling pathways in CHF. Further studies should investigate differences in the biochemical processes leading to heart failure. MDPI 2019-11-29 /pmc/articles/PMC6956299/ /pubmed/31795484 http://dx.doi.org/10.3390/medicina55120766 Text en © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Communication
Komici, Klara
Gnemmi, Isabella
Sangiorgi, Claudia
Ricciardolo, Fabio Luigi Massimo
Rinaldi, Mauro
Di Stefano, Antonino
Eleuteri, Ermanno
Indexes of Angiogenic Activation in Myocardial Samples of Patients with Advanced Chronic Heart Failure
title Indexes of Angiogenic Activation in Myocardial Samples of Patients with Advanced Chronic Heart Failure
title_full Indexes of Angiogenic Activation in Myocardial Samples of Patients with Advanced Chronic Heart Failure
title_fullStr Indexes of Angiogenic Activation in Myocardial Samples of Patients with Advanced Chronic Heart Failure
title_full_unstemmed Indexes of Angiogenic Activation in Myocardial Samples of Patients with Advanced Chronic Heart Failure
title_short Indexes of Angiogenic Activation in Myocardial Samples of Patients with Advanced Chronic Heart Failure
title_sort indexes of angiogenic activation in myocardial samples of patients with advanced chronic heart failure
topic Communication
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6956299/
https://www.ncbi.nlm.nih.gov/pubmed/31795484
http://dx.doi.org/10.3390/medicina55120766
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