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Involvement of the TGF-β1 pathway in caveolin-1-associated regulation of head and neck tumor cell metastasis
Head and neck squamous cell carcinoma (HNSCC) is the sixth most frequent malignancy with a 5-year survival rate of 54%. Therefore, disease management improvement is required. The present study aimed to assess the role of caveolin-1 (Cav-1) in the metastasis of head and neck tumor cells. Short hairpi...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
D.A. Spandidos
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6956420/ https://www.ncbi.nlm.nih.gov/pubmed/31966060 http://dx.doi.org/10.3892/ol.2019.11187 |
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author | Sun, Jinjie Lu, Yongtian Yu, Changyun Xu, Ting Nie, Guohui Miao, Beiping Zhang, Xin |
author_facet | Sun, Jinjie Lu, Yongtian Yu, Changyun Xu, Ting Nie, Guohui Miao, Beiping Zhang, Xin |
author_sort | Sun, Jinjie |
collection | PubMed |
description | Head and neck squamous cell carcinoma (HNSCC) is the sixth most frequent malignancy with a 5-year survival rate of 54%. Therefore, disease management improvement is required. The present study aimed to assess the role of caveolin-1 (Cav-1) in the metastasis of head and neck tumor cells. Short hairpin RNA was used to silence Cav-1 expression in Tu686 cells. Proliferation, migration, invasion, morphology and the levels of effector proteins were assessed in cells. Upon Cav-1 silencing, E-cadherin levels were decreased, while vimentin levels were significantly increased. Cell migration, quantified by wound healing and Transwell assays, was significantly increased. Meanwhile, Cav-1 and transforming growth factor β1 (TGF-β1) receptor were identified to be co-localized. In addition, Cav-1-knockdown resulted in increased phosphorylation of SMAD family member 2 (P<0.05), a downstream effector of TGF-β signaling. In addition, there was a mutual regulation, with increasing TGF-β1 levels leading to a dose-dependent decrease of Cav-1 expression levels (P<0.05). These findings indicate that Cav-1 inhibits cell metastasis in HNSCC, suggesting the involvement of the TGF-β signaling pathway. |
format | Online Article Text |
id | pubmed-6956420 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | D.A. Spandidos |
record_format | MEDLINE/PubMed |
spelling | pubmed-69564202020-01-21 Involvement of the TGF-β1 pathway in caveolin-1-associated regulation of head and neck tumor cell metastasis Sun, Jinjie Lu, Yongtian Yu, Changyun Xu, Ting Nie, Guohui Miao, Beiping Zhang, Xin Oncol Lett Articles Head and neck squamous cell carcinoma (HNSCC) is the sixth most frequent malignancy with a 5-year survival rate of 54%. Therefore, disease management improvement is required. The present study aimed to assess the role of caveolin-1 (Cav-1) in the metastasis of head and neck tumor cells. Short hairpin RNA was used to silence Cav-1 expression in Tu686 cells. Proliferation, migration, invasion, morphology and the levels of effector proteins were assessed in cells. Upon Cav-1 silencing, E-cadherin levels were decreased, while vimentin levels were significantly increased. Cell migration, quantified by wound healing and Transwell assays, was significantly increased. Meanwhile, Cav-1 and transforming growth factor β1 (TGF-β1) receptor were identified to be co-localized. In addition, Cav-1-knockdown resulted in increased phosphorylation of SMAD family member 2 (P<0.05), a downstream effector of TGF-β signaling. In addition, there was a mutual regulation, with increasing TGF-β1 levels leading to a dose-dependent decrease of Cav-1 expression levels (P<0.05). These findings indicate that Cav-1 inhibits cell metastasis in HNSCC, suggesting the involvement of the TGF-β signaling pathway. D.A. Spandidos 2020-02 2019-12-06 /pmc/articles/PMC6956420/ /pubmed/31966060 http://dx.doi.org/10.3892/ol.2019.11187 Text en Copyright: © Sun et al. This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License (https://creativecommons.org/licenses/by-nc-nd/4.0/) , which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made. |
spellingShingle | Articles Sun, Jinjie Lu, Yongtian Yu, Changyun Xu, Ting Nie, Guohui Miao, Beiping Zhang, Xin Involvement of the TGF-β1 pathway in caveolin-1-associated regulation of head and neck tumor cell metastasis |
title | Involvement of the TGF-β1 pathway in caveolin-1-associated regulation of head and neck tumor cell metastasis |
title_full | Involvement of the TGF-β1 pathway in caveolin-1-associated regulation of head and neck tumor cell metastasis |
title_fullStr | Involvement of the TGF-β1 pathway in caveolin-1-associated regulation of head and neck tumor cell metastasis |
title_full_unstemmed | Involvement of the TGF-β1 pathway in caveolin-1-associated regulation of head and neck tumor cell metastasis |
title_short | Involvement of the TGF-β1 pathway in caveolin-1-associated regulation of head and neck tumor cell metastasis |
title_sort | involvement of the tgf-β1 pathway in caveolin-1-associated regulation of head and neck tumor cell metastasis |
topic | Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6956420/ https://www.ncbi.nlm.nih.gov/pubmed/31966060 http://dx.doi.org/10.3892/ol.2019.11187 |
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