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Odor-Induced Multi-Level Inhibitory Maps in Drosophila

Optical imaging of intracellular Ca(2+) influx as a correlate of neuronal excitation represents a standard technique for visualizing spatiotemporal activity of neuronal networks. However, the information-processing properties of single neurons and neuronal circuits likewise involve inhibition of neu...

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Detalles Bibliográficos
Autores principales: Grabe, Veit, Schubert, Marco, Strube-Bloss, Martin, Reinert, Anja, Trautheim, Silke, Lavista-Llanos, Sofia, Fiala, André, Hansson, Bill S., Sachse, Silke
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Society for Neuroscience 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6957311/
https://www.ncbi.nlm.nih.gov/pubmed/31888962
http://dx.doi.org/10.1523/ENEURO.0213-19.2019
Descripción
Sumario:Optical imaging of intracellular Ca(2+) influx as a correlate of neuronal excitation represents a standard technique for visualizing spatiotemporal activity of neuronal networks. However, the information-processing properties of single neurons and neuronal circuits likewise involve inhibition of neuronal membrane potential. Here, we report spatially resolved optical imaging of odor-evoked inhibitory patterns in the olfactory circuitry of Drosophila using a genetically encoded fluorescent Cl(-) sensor. In combination with the excitatory component reflected by intracellular Ca(2+) dynamics, we present a comprehensive functional map of both odor-evoked neuronal activation and inhibition at different levels of olfactory processing. We demonstrate that odor-evoked inhibition carried by Cl(-) influx is present both in sensory neurons and second-order projection neurons (PNs), and is characterized by stereotypic, odor-specific patterns. Cl(-)-mediated inhibition features distinct dynamics in different neuronal populations. Our data support a dual role of inhibitory neurons in the olfactory system: global gain control across the neuronal circuitry and glomerulus-specific inhibition to enhance neuronal information processing.