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Two novel proteins, TtpB2 and TtpD2, are essential for iron transport in the TonB2 system of Vibrio vulnificus

In gram‐negative bacteria, energy‐dependent active transport of iron‐bound substrates across the outer membrane is achieved through the TonB systems of proteins. Three TonB systems have been identified in the human pathogen Vibrio vulnificus. The TonB1 system contains three proteins: TonB1, ExbB1, a...

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Autores principales: Barnes, Adel D., Pfeifer, Hailey J., Zbylicki, Brianne R., Roberts, Elena K., Rudd, Justin C., Manzo, Mario A., Phillips, Elysse A., Berry, Michael M., Kenton, Ryan J.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6957404/
https://www.ncbi.nlm.nih.gov/pubmed/31595707
http://dx.doi.org/10.1002/mbo3.947
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author Barnes, Adel D.
Pfeifer, Hailey J.
Zbylicki, Brianne R.
Roberts, Elena K.
Rudd, Justin C.
Manzo, Mario A.
Phillips, Elysse A.
Berry, Michael M.
Kenton, Ryan J.
author_facet Barnes, Adel D.
Pfeifer, Hailey J.
Zbylicki, Brianne R.
Roberts, Elena K.
Rudd, Justin C.
Manzo, Mario A.
Phillips, Elysse A.
Berry, Michael M.
Kenton, Ryan J.
author_sort Barnes, Adel D.
collection PubMed
description In gram‐negative bacteria, energy‐dependent active transport of iron‐bound substrates across the outer membrane is achieved through the TonB systems of proteins. Three TonB systems have been identified in the human pathogen Vibrio vulnificus. The TonB1 system contains three proteins: TonB1, ExbB1, and ExbD1. Both the TonB2 and TonB3 systems have been shown to also contain a fourth protein, TtpC2 and TtpC3, respectively. Here, we report and begin to characterize two additional proteins in the TonB2 and TonB3 systems: TtpB and TtpD. Both TtpB2 and TtpD2 are absolutely required for the function of the TonB2 system in V. vulnificus. However, although both TtpB3 and TtpD3 in the TonB3 system are related to the proteins in the TonB2 system, neither are active in iron transport. All six protein components of the TonB2 system—TonB2, ExbB2, ExbD2, TtpB2, TtpC2, and TtpD2—are essential for the uptake of both endogenously produced iron‐bound siderophores and exogenous siderophores produced from other organisms. Through complementation, we have shown that V. vulnificus is capable of using different TtpD2 proteins from other Vibrio species to bring in multiple siderophores. In contrast, we also demonstrate that TtpB2 must come from V. vulnificus, and not other species within the genus, to complement mutations in the TonB2 system.
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spelling pubmed-69574042020-01-17 Two novel proteins, TtpB2 and TtpD2, are essential for iron transport in the TonB2 system of Vibrio vulnificus Barnes, Adel D. Pfeifer, Hailey J. Zbylicki, Brianne R. Roberts, Elena K. Rudd, Justin C. Manzo, Mario A. Phillips, Elysse A. Berry, Michael M. Kenton, Ryan J. Microbiologyopen Original Articles In gram‐negative bacteria, energy‐dependent active transport of iron‐bound substrates across the outer membrane is achieved through the TonB systems of proteins. Three TonB systems have been identified in the human pathogen Vibrio vulnificus. The TonB1 system contains three proteins: TonB1, ExbB1, and ExbD1. Both the TonB2 and TonB3 systems have been shown to also contain a fourth protein, TtpC2 and TtpC3, respectively. Here, we report and begin to characterize two additional proteins in the TonB2 and TonB3 systems: TtpB and TtpD. Both TtpB2 and TtpD2 are absolutely required for the function of the TonB2 system in V. vulnificus. However, although both TtpB3 and TtpD3 in the TonB3 system are related to the proteins in the TonB2 system, neither are active in iron transport. All six protein components of the TonB2 system—TonB2, ExbB2, ExbD2, TtpB2, TtpC2, and TtpD2—are essential for the uptake of both endogenously produced iron‐bound siderophores and exogenous siderophores produced from other organisms. Through complementation, we have shown that V. vulnificus is capable of using different TtpD2 proteins from other Vibrio species to bring in multiple siderophores. In contrast, we also demonstrate that TtpB2 must come from V. vulnificus, and not other species within the genus, to complement mutations in the TonB2 system. John Wiley and Sons Inc. 2019-10-08 /pmc/articles/PMC6957404/ /pubmed/31595707 http://dx.doi.org/10.1002/mbo3.947 Text en © 2019 The Authors. MicrobiologyOpen published by John Wiley & Sons Ltd. This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
spellingShingle Original Articles
Barnes, Adel D.
Pfeifer, Hailey J.
Zbylicki, Brianne R.
Roberts, Elena K.
Rudd, Justin C.
Manzo, Mario A.
Phillips, Elysse A.
Berry, Michael M.
Kenton, Ryan J.
Two novel proteins, TtpB2 and TtpD2, are essential for iron transport in the TonB2 system of Vibrio vulnificus
title Two novel proteins, TtpB2 and TtpD2, are essential for iron transport in the TonB2 system of Vibrio vulnificus
title_full Two novel proteins, TtpB2 and TtpD2, are essential for iron transport in the TonB2 system of Vibrio vulnificus
title_fullStr Two novel proteins, TtpB2 and TtpD2, are essential for iron transport in the TonB2 system of Vibrio vulnificus
title_full_unstemmed Two novel proteins, TtpB2 and TtpD2, are essential for iron transport in the TonB2 system of Vibrio vulnificus
title_short Two novel proteins, TtpB2 and TtpD2, are essential for iron transport in the TonB2 system of Vibrio vulnificus
title_sort two novel proteins, ttpb2 and ttpd2, are essential for iron transport in the tonb2 system of vibrio vulnificus
topic Original Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6957404/
https://www.ncbi.nlm.nih.gov/pubmed/31595707
http://dx.doi.org/10.1002/mbo3.947
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