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Separation and Purification of Lipase Inhibitory Peptide from Fermented Milk by Lactobacillus plantarum Q180
In this study, we separated and purified lipase inhibitory peptide from fermented milk by Lactobacillus plantarum Q180 with the aim of developing a new functional anti-lipase activity yogurt product. L. plantarum 180 was inoculated into 10% reconstituted skimmed milk and incubated at 37°C until the...
Autores principales: | , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Korean Society for Food Science of Animal Resources
2020
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6957449/ https://www.ncbi.nlm.nih.gov/pubmed/31970333 http://dx.doi.org/10.5851/kosfa.2019.e87 |
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author | Kim, Seulki Lim, Sang-Dong |
author_facet | Kim, Seulki Lim, Sang-Dong |
author_sort | Kim, Seulki |
collection | PubMed |
description | In this study, we separated and purified lipase inhibitory peptide from fermented milk by Lactobacillus plantarum Q180 with the aim of developing a new functional anti-lipase activity yogurt product. L. plantarum 180 was inoculated into 10% reconstituted skimmed milk and incubated at 37°C until the pH of the culture reached pH 4.4. The lipase activity was measured using porcine pancreatic lipase. The lipase inhibitory peptides were gradually isolated by ultrafiltration, reversed phase column chromatography (RPC), reversed phase high-performance liquid chromatography (RP-HPLC), and gel permeation high-performance liquid chromatography (GP-HPLC) from the fermented milk by L. plantarum Q180. An ODS-AQ column was used for the RPC, a Vydac C18 column for the RP-HPLC, and a Superdex Peptide HR column for the GP-HPLC. The peptide was composed of Asp, Thr, Ile, Ser, Ala, and Gln, and the anti-lipase activity (IC(50)) was 2,817 μg/mL. |
format | Online Article Text |
id | pubmed-6957449 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | Korean Society for Food Science of Animal Resources |
record_format | MEDLINE/PubMed |
spelling | pubmed-69574492020-01-22 Separation and Purification of Lipase Inhibitory Peptide from Fermented Milk by Lactobacillus plantarum Q180 Kim, Seulki Lim, Sang-Dong Food Sci Anim Resour Article In this study, we separated and purified lipase inhibitory peptide from fermented milk by Lactobacillus plantarum Q180 with the aim of developing a new functional anti-lipase activity yogurt product. L. plantarum 180 was inoculated into 10% reconstituted skimmed milk and incubated at 37°C until the pH of the culture reached pH 4.4. The lipase activity was measured using porcine pancreatic lipase. The lipase inhibitory peptides were gradually isolated by ultrafiltration, reversed phase column chromatography (RPC), reversed phase high-performance liquid chromatography (RP-HPLC), and gel permeation high-performance liquid chromatography (GP-HPLC) from the fermented milk by L. plantarum Q180. An ODS-AQ column was used for the RPC, a Vydac C18 column for the RP-HPLC, and a Superdex Peptide HR column for the GP-HPLC. The peptide was composed of Asp, Thr, Ile, Ser, Ala, and Gln, and the anti-lipase activity (IC(50)) was 2,817 μg/mL. Korean Society for Food Science of Animal Resources 2020-01 2020-01-01 /pmc/articles/PMC6957449/ /pubmed/31970333 http://dx.doi.org/10.5851/kosfa.2019.e87 Text en © Korean Society for Food Science of Animal Resources http://creativecommons.org/licenses/by-nc/3.0/ This is an Open-Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Article Kim, Seulki Lim, Sang-Dong Separation and Purification of Lipase Inhibitory Peptide from Fermented Milk by Lactobacillus plantarum Q180 |
title | Separation and Purification of Lipase Inhibitory Peptide from
Fermented Milk by Lactobacillus plantarum Q180 |
title_full | Separation and Purification of Lipase Inhibitory Peptide from
Fermented Milk by Lactobacillus plantarum Q180 |
title_fullStr | Separation and Purification of Lipase Inhibitory Peptide from
Fermented Milk by Lactobacillus plantarum Q180 |
title_full_unstemmed | Separation and Purification of Lipase Inhibitory Peptide from
Fermented Milk by Lactobacillus plantarum Q180 |
title_short | Separation and Purification of Lipase Inhibitory Peptide from
Fermented Milk by Lactobacillus plantarum Q180 |
title_sort | separation and purification of lipase inhibitory peptide from
fermented milk by lactobacillus plantarum q180 |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6957449/ https://www.ncbi.nlm.nih.gov/pubmed/31970333 http://dx.doi.org/10.5851/kosfa.2019.e87 |
work_keys_str_mv | AT kimseulki separationandpurificationoflipaseinhibitorypeptidefromfermentedmilkbylactobacillusplantarumq180 AT limsangdong separationandpurificationoflipaseinhibitorypeptidefromfermentedmilkbylactobacillusplantarumq180 |