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Programmed magnetic manipulation of vesicles into spatially coded prototissue architectures arrays
In nature, cells self-assemble into spatially coded tissular configurations to execute higher-order biological functions as a collective. This mechanism has stimulated the recent trend in synthetic biology to construct tissue-like assemblies from protocell entities, with the aim to understand the ev...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group UK
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6957477/ https://www.ncbi.nlm.nih.gov/pubmed/31932592 http://dx.doi.org/10.1038/s41467-019-14141-x |
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author | Li, Qingchuan Li, Shubin Zhang, Xiangxiang Xu, Weili Han, Xiaojun |
author_facet | Li, Qingchuan Li, Shubin Zhang, Xiangxiang Xu, Weili Han, Xiaojun |
author_sort | Li, Qingchuan |
collection | PubMed |
description | In nature, cells self-assemble into spatially coded tissular configurations to execute higher-order biological functions as a collective. This mechanism has stimulated the recent trend in synthetic biology to construct tissue-like assemblies from protocell entities, with the aim to understand the evolution mechanism of multicellular mechanisms, create smart materials or devices, and engineer tissue-like biomedical implant. However, the formation of spatially coded and communicating micro-architectures from large quantity of protocell entities, especially for lipid vesicle-based systems that mostly resemble cells, is still challenging. Herein, we magnetically assemble giant unilamellar vesicles (GUVs) or cells into various microstructures with spatially coded configurations and spatialized cascade biochemical reactions using a stainless steel mesh. GUVs in these tissue-like aggregates exhibit uncustomary osmotic stability that cannot be achieved by individual GUVs suspensions. This work provides a versatile and cost-effective strategy to form robust tissue-mimics and indicates a possible superiority of protocell colonies to individual protocells. |
format | Online Article Text |
id | pubmed-6957477 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | Nature Publishing Group UK |
record_format | MEDLINE/PubMed |
spelling | pubmed-69574772020-01-15 Programmed magnetic manipulation of vesicles into spatially coded prototissue architectures arrays Li, Qingchuan Li, Shubin Zhang, Xiangxiang Xu, Weili Han, Xiaojun Nat Commun Article In nature, cells self-assemble into spatially coded tissular configurations to execute higher-order biological functions as a collective. This mechanism has stimulated the recent trend in synthetic biology to construct tissue-like assemblies from protocell entities, with the aim to understand the evolution mechanism of multicellular mechanisms, create smart materials or devices, and engineer tissue-like biomedical implant. However, the formation of spatially coded and communicating micro-architectures from large quantity of protocell entities, especially for lipid vesicle-based systems that mostly resemble cells, is still challenging. Herein, we magnetically assemble giant unilamellar vesicles (GUVs) or cells into various microstructures with spatially coded configurations and spatialized cascade biochemical reactions using a stainless steel mesh. GUVs in these tissue-like aggregates exhibit uncustomary osmotic stability that cannot be achieved by individual GUVs suspensions. This work provides a versatile and cost-effective strategy to form robust tissue-mimics and indicates a possible superiority of protocell colonies to individual protocells. Nature Publishing Group UK 2020-01-13 /pmc/articles/PMC6957477/ /pubmed/31932592 http://dx.doi.org/10.1038/s41467-019-14141-x Text en © The Author(s) 2020 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/. |
spellingShingle | Article Li, Qingchuan Li, Shubin Zhang, Xiangxiang Xu, Weili Han, Xiaojun Programmed magnetic manipulation of vesicles into spatially coded prototissue architectures arrays |
title | Programmed magnetic manipulation of vesicles into spatially coded prototissue architectures arrays |
title_full | Programmed magnetic manipulation of vesicles into spatially coded prototissue architectures arrays |
title_fullStr | Programmed magnetic manipulation of vesicles into spatially coded prototissue architectures arrays |
title_full_unstemmed | Programmed magnetic manipulation of vesicles into spatially coded prototissue architectures arrays |
title_short | Programmed magnetic manipulation of vesicles into spatially coded prototissue architectures arrays |
title_sort | programmed magnetic manipulation of vesicles into spatially coded prototissue architectures arrays |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6957477/ https://www.ncbi.nlm.nih.gov/pubmed/31932592 http://dx.doi.org/10.1038/s41467-019-14141-x |
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