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Antinociceptive activity of methanol extract of Chlorophytum alismifolium tubers in murine model of pain: Possible involvement of α(2)-adrenergic receptor and K(ATP) channels
The tubers of Chlorophytum alismifolium are used in Nigerian Herbal Medicine for the management of diabetes mellitus, painful and inflammatory conditions. The antinociceptive activity has been validated but the mechanism of this activity is yet to be explored. This study therefore, aimed to investig...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Elsevier
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6957804/ https://www.ncbi.nlm.nih.gov/pubmed/31956552 http://dx.doi.org/10.1016/j.jtcme.2019.03.005 |
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author | Abubakar, Abdulhakim Nazifi, Abdullahi Balarabe Odoma, Saidi Shehu, Salisu Danjuma, Nuhu Mohammed |
author_facet | Abubakar, Abdulhakim Nazifi, Abdullahi Balarabe Odoma, Saidi Shehu, Salisu Danjuma, Nuhu Mohammed |
author_sort | Abubakar, Abdulhakim |
collection | PubMed |
description | The tubers of Chlorophytum alismifolium are used in Nigerian Herbal Medicine for the management of diabetes mellitus, painful and inflammatory conditions. The antinociceptive activity has been validated but the mechanism of this activity is yet to be explored. This study therefore, aimed to investigate the probable mechanism(s) of the antinociceptive activity of C. alismifolium tubers using experimental animal model of pain. HPLC and GC-MS analyses were carried out on the extract. Antinociceptive activity was investigated using acetic acid-induced writhing response test in mice. Three groups of mice were orally administered distilled water (10 ml/kg), C. alismifolium (400 mg/kg) and morphine (10 mg/kg) 60 min before administration of acetic acid and the resulting writhing were counted for 10 min. To establish the probable mechanism(s) of action of C. alismifolium, separate groups of animals were pretreated intraperitoneally with naloxone (2 mg/kg), prazosin (1 mg/kg), yohimbine (1 mg/kg), propranolol (20 mg/kg) and glibenclamide (5 mg/kg) 15 min before C. alismifolium administration. HPLC chromatogram of the extract revealed seventeen characteristic peaks with retention times ranging between 2.1 and 7.4 min. Administration of C. alismifolium significantly (p < 0.01) reduced the mean number of writhes compared to control group. Pretreatment with yohimbine and glibenclamide significantly (p < 0.05 and p < 0.01 respectively) reduced the antinociceptive activity of extract-alone treated group. However, pretreatment with prazosin, naloxone and propranolol showed no effect on its analgesic activity. The findings from this research revealed the possible involvement of α(2)-adrenergic receptor and K(ATP) channels in the antinociceptive activity of Chlorophytum alismifolium tuber extract. |
format | Online Article Text |
id | pubmed-6957804 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | Elsevier |
record_format | MEDLINE/PubMed |
spelling | pubmed-69578042020-01-17 Antinociceptive activity of methanol extract of Chlorophytum alismifolium tubers in murine model of pain: Possible involvement of α(2)-adrenergic receptor and K(ATP) channels Abubakar, Abdulhakim Nazifi, Abdullahi Balarabe Odoma, Saidi Shehu, Salisu Danjuma, Nuhu Mohammed J Tradit Complement Med Short Communication The tubers of Chlorophytum alismifolium are used in Nigerian Herbal Medicine for the management of diabetes mellitus, painful and inflammatory conditions. The antinociceptive activity has been validated but the mechanism of this activity is yet to be explored. This study therefore, aimed to investigate the probable mechanism(s) of the antinociceptive activity of C. alismifolium tubers using experimental animal model of pain. HPLC and GC-MS analyses were carried out on the extract. Antinociceptive activity was investigated using acetic acid-induced writhing response test in mice. Three groups of mice were orally administered distilled water (10 ml/kg), C. alismifolium (400 mg/kg) and morphine (10 mg/kg) 60 min before administration of acetic acid and the resulting writhing were counted for 10 min. To establish the probable mechanism(s) of action of C. alismifolium, separate groups of animals were pretreated intraperitoneally with naloxone (2 mg/kg), prazosin (1 mg/kg), yohimbine (1 mg/kg), propranolol (20 mg/kg) and glibenclamide (5 mg/kg) 15 min before C. alismifolium administration. HPLC chromatogram of the extract revealed seventeen characteristic peaks with retention times ranging between 2.1 and 7.4 min. Administration of C. alismifolium significantly (p < 0.01) reduced the mean number of writhes compared to control group. Pretreatment with yohimbine and glibenclamide significantly (p < 0.05 and p < 0.01 respectively) reduced the antinociceptive activity of extract-alone treated group. However, pretreatment with prazosin, naloxone and propranolol showed no effect on its analgesic activity. The findings from this research revealed the possible involvement of α(2)-adrenergic receptor and K(ATP) channels in the antinociceptive activity of Chlorophytum alismifolium tuber extract. Elsevier 2019-03-16 /pmc/articles/PMC6957804/ /pubmed/31956552 http://dx.doi.org/10.1016/j.jtcme.2019.03.005 Text en © 2019 Center for Food and Biomolecules, National Taiwan University. Production and hosting by Elsevier Taiwan LLC. http://creativecommons.org/licenses/by-nc-nd/4.0/ This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/). |
spellingShingle | Short Communication Abubakar, Abdulhakim Nazifi, Abdullahi Balarabe Odoma, Saidi Shehu, Salisu Danjuma, Nuhu Mohammed Antinociceptive activity of methanol extract of Chlorophytum alismifolium tubers in murine model of pain: Possible involvement of α(2)-adrenergic receptor and K(ATP) channels |
title | Antinociceptive activity of methanol extract of Chlorophytum alismifolium tubers in murine model of pain: Possible involvement of α(2)-adrenergic receptor and K(ATP) channels |
title_full | Antinociceptive activity of methanol extract of Chlorophytum alismifolium tubers in murine model of pain: Possible involvement of α(2)-adrenergic receptor and K(ATP) channels |
title_fullStr | Antinociceptive activity of methanol extract of Chlorophytum alismifolium tubers in murine model of pain: Possible involvement of α(2)-adrenergic receptor and K(ATP) channels |
title_full_unstemmed | Antinociceptive activity of methanol extract of Chlorophytum alismifolium tubers in murine model of pain: Possible involvement of α(2)-adrenergic receptor and K(ATP) channels |
title_short | Antinociceptive activity of methanol extract of Chlorophytum alismifolium tubers in murine model of pain: Possible involvement of α(2)-adrenergic receptor and K(ATP) channels |
title_sort | antinociceptive activity of methanol extract of chlorophytum alismifolium tubers in murine model of pain: possible involvement of α(2)-adrenergic receptor and k(atp) channels |
topic | Short Communication |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6957804/ https://www.ncbi.nlm.nih.gov/pubmed/31956552 http://dx.doi.org/10.1016/j.jtcme.2019.03.005 |
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