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Molecular Diagnostics of Mucormycosis in Hematological Patients: A Literature Review

Objectives: to analyze the results of molecular methods applying for the diagnosis of mucormycosis in hematologic patients based on a literature review. Data sources: A systematic search in databases PubMed, Google Scholar for August 2019. Review eligibility criteria: original articles published in...

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Autores principales: Shadrivova, Olga V., Burygina, Ekaterina V., Klimko, Nikolai N.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6958327/
https://www.ncbi.nlm.nih.gov/pubmed/31795369
http://dx.doi.org/10.3390/jof5040112
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author Shadrivova, Olga V.
Burygina, Ekaterina V.
Klimko, Nikolai N.
author_facet Shadrivova, Olga V.
Burygina, Ekaterina V.
Klimko, Nikolai N.
author_sort Shadrivova, Olga V.
collection PubMed
description Objectives: to analyze the results of molecular methods applying for the diagnosis of mucormycosis in hematologic patients based on a literature review. Data sources: A systematic search in databases PubMed, Google Scholar for August 2019. Review eligibility criteria: original articles published in English, studies of molecular methods for the diagnosis of mucormycosis in hematologic patients. Results. We analyzed the research data from 116 hematological patients with mucormycosis, including children (6%). Patients with localized forms of mucormycosis prevailed (72%), and lung involvement was diagnosed in 58% of these cases. For molecular verification of the causative agent of mucormycosis, blood serum was most often used, less commonly postoperative and autopsy material, biopsy specimens, formalin-fixed paraffin-embedded samples and bronchoalveolar lavage, pleural fluid and sputum. The sensitivity of molecular diagnostics of mucormycosis in a cohort of hematological patients was 88.2%. Conclusion. The use of molecular techniques along with standard mycological methods will improve the diagnostics of mucormycosis in hematologic patients. However, prospective studies of the effectiveness of molecular methods for the diagnosis of mucormycosis of various etiologies in hematological patients, including children, using bronchoalveolar lavage (BAL) and cerebrospinal fluid (CSF) are needed.
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spelling pubmed-69583272020-01-23 Molecular Diagnostics of Mucormycosis in Hematological Patients: A Literature Review Shadrivova, Olga V. Burygina, Ekaterina V. Klimko, Nikolai N. J Fungi (Basel) Review Objectives: to analyze the results of molecular methods applying for the diagnosis of mucormycosis in hematologic patients based on a literature review. Data sources: A systematic search in databases PubMed, Google Scholar for August 2019. Review eligibility criteria: original articles published in English, studies of molecular methods for the diagnosis of mucormycosis in hematologic patients. Results. We analyzed the research data from 116 hematological patients with mucormycosis, including children (6%). Patients with localized forms of mucormycosis prevailed (72%), and lung involvement was diagnosed in 58% of these cases. For molecular verification of the causative agent of mucormycosis, blood serum was most often used, less commonly postoperative and autopsy material, biopsy specimens, formalin-fixed paraffin-embedded samples and bronchoalveolar lavage, pleural fluid and sputum. The sensitivity of molecular diagnostics of mucormycosis in a cohort of hematological patients was 88.2%. Conclusion. The use of molecular techniques along with standard mycological methods will improve the diagnostics of mucormycosis in hematologic patients. However, prospective studies of the effectiveness of molecular methods for the diagnosis of mucormycosis of various etiologies in hematological patients, including children, using bronchoalveolar lavage (BAL) and cerebrospinal fluid (CSF) are needed. MDPI 2019-11-29 /pmc/articles/PMC6958327/ /pubmed/31795369 http://dx.doi.org/10.3390/jof5040112 Text en © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Review
Shadrivova, Olga V.
Burygina, Ekaterina V.
Klimko, Nikolai N.
Molecular Diagnostics of Mucormycosis in Hematological Patients: A Literature Review
title Molecular Diagnostics of Mucormycosis in Hematological Patients: A Literature Review
title_full Molecular Diagnostics of Mucormycosis in Hematological Patients: A Literature Review
title_fullStr Molecular Diagnostics of Mucormycosis in Hematological Patients: A Literature Review
title_full_unstemmed Molecular Diagnostics of Mucormycosis in Hematological Patients: A Literature Review
title_short Molecular Diagnostics of Mucormycosis in Hematological Patients: A Literature Review
title_sort molecular diagnostics of mucormycosis in hematological patients: a literature review
topic Review
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6958327/
https://www.ncbi.nlm.nih.gov/pubmed/31795369
http://dx.doi.org/10.3390/jof5040112
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