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Connexin 26 and Connexin 43 in Canine Mammary Carcinoma

Incidence of canine mammary carcinoma is two times higher than the rate of human breast cancer. Mammary tumors are the most common type of cancer in intact female dogs and account for about half of all neoplasms in these dogs. Well-established models of breast cancer have shown that neoplastic cells...

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Autores principales: Luu, Savannah, Bell, Cynthia, Schneider, Sarah, Nguyen, Thu Annelise
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6958330/
https://www.ncbi.nlm.nih.gov/pubmed/31818036
http://dx.doi.org/10.3390/vetsci6040101
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author Luu, Savannah
Bell, Cynthia
Schneider, Sarah
Nguyen, Thu Annelise
author_facet Luu, Savannah
Bell, Cynthia
Schneider, Sarah
Nguyen, Thu Annelise
author_sort Luu, Savannah
collection PubMed
description Incidence of canine mammary carcinoma is two times higher than the rate of human breast cancer. Mammary tumors are the most common type of cancer in intact female dogs and account for about half of all neoplasms in these dogs. Well-established models of breast cancer have shown that neoplastic cells often have a loss of intercellular communication, particularly gap junction proteins. Thus, the objective of this study is to explore the aspect of gap junction intercellular communication in canine mammary carcinoma, non-cancerous (CMEC) and cancerous (CMT12, CMT27, and CF41.Mg) cells, and patient-derived tumors. Both non-cancerous and cancerous mammary cells express connexins 26 and 43 using immunofluorescence; however, the level of expression is significantly different in quantitative analysis using western blot in which connexin 43 in both CMT12 and CMT27 is significantly decreased compared to CMEC. Furthermore, a decrease of gap junction capacity in CMT12 and CMT27 was observed compared to CMEC. Immunostaining of CMT27-xenograft tumors revealed positive Cx26 and negative Cx43 expression. Similarly, immunostaining of spontaneous canine mammary tumors revealed that Cx26 is present in all tumors while Cx43 is present in 25% of tumors. Overall, the study provides for the first time that a differential pattern of connexin expression exists between non-cancerous and cancerous mammary cells in dogs. This study will pave the path for further in vitro work of connexins in comparative canine models and possibly allow for novel therapeutics to be developed.
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spelling pubmed-69583302020-01-23 Connexin 26 and Connexin 43 in Canine Mammary Carcinoma Luu, Savannah Bell, Cynthia Schneider, Sarah Nguyen, Thu Annelise Vet Sci Article Incidence of canine mammary carcinoma is two times higher than the rate of human breast cancer. Mammary tumors are the most common type of cancer in intact female dogs and account for about half of all neoplasms in these dogs. Well-established models of breast cancer have shown that neoplastic cells often have a loss of intercellular communication, particularly gap junction proteins. Thus, the objective of this study is to explore the aspect of gap junction intercellular communication in canine mammary carcinoma, non-cancerous (CMEC) and cancerous (CMT12, CMT27, and CF41.Mg) cells, and patient-derived tumors. Both non-cancerous and cancerous mammary cells express connexins 26 and 43 using immunofluorescence; however, the level of expression is significantly different in quantitative analysis using western blot in which connexin 43 in both CMT12 and CMT27 is significantly decreased compared to CMEC. Furthermore, a decrease of gap junction capacity in CMT12 and CMT27 was observed compared to CMEC. Immunostaining of CMT27-xenograft tumors revealed positive Cx26 and negative Cx43 expression. Similarly, immunostaining of spontaneous canine mammary tumors revealed that Cx26 is present in all tumors while Cx43 is present in 25% of tumors. Overall, the study provides for the first time that a differential pattern of connexin expression exists between non-cancerous and cancerous mammary cells in dogs. This study will pave the path for further in vitro work of connexins in comparative canine models and possibly allow for novel therapeutics to be developed. MDPI 2019-12-09 /pmc/articles/PMC6958330/ /pubmed/31818036 http://dx.doi.org/10.3390/vetsci6040101 Text en © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Luu, Savannah
Bell, Cynthia
Schneider, Sarah
Nguyen, Thu Annelise
Connexin 26 and Connexin 43 in Canine Mammary Carcinoma
title Connexin 26 and Connexin 43 in Canine Mammary Carcinoma
title_full Connexin 26 and Connexin 43 in Canine Mammary Carcinoma
title_fullStr Connexin 26 and Connexin 43 in Canine Mammary Carcinoma
title_full_unstemmed Connexin 26 and Connexin 43 in Canine Mammary Carcinoma
title_short Connexin 26 and Connexin 43 in Canine Mammary Carcinoma
title_sort connexin 26 and connexin 43 in canine mammary carcinoma
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6958330/
https://www.ncbi.nlm.nih.gov/pubmed/31818036
http://dx.doi.org/10.3390/vetsci6040101
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