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Connexin 26 and Connexin 43 in Canine Mammary Carcinoma
Incidence of canine mammary carcinoma is two times higher than the rate of human breast cancer. Mammary tumors are the most common type of cancer in intact female dogs and account for about half of all neoplasms in these dogs. Well-established models of breast cancer have shown that neoplastic cells...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6958330/ https://www.ncbi.nlm.nih.gov/pubmed/31818036 http://dx.doi.org/10.3390/vetsci6040101 |
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author | Luu, Savannah Bell, Cynthia Schneider, Sarah Nguyen, Thu Annelise |
author_facet | Luu, Savannah Bell, Cynthia Schneider, Sarah Nguyen, Thu Annelise |
author_sort | Luu, Savannah |
collection | PubMed |
description | Incidence of canine mammary carcinoma is two times higher than the rate of human breast cancer. Mammary tumors are the most common type of cancer in intact female dogs and account for about half of all neoplasms in these dogs. Well-established models of breast cancer have shown that neoplastic cells often have a loss of intercellular communication, particularly gap junction proteins. Thus, the objective of this study is to explore the aspect of gap junction intercellular communication in canine mammary carcinoma, non-cancerous (CMEC) and cancerous (CMT12, CMT27, and CF41.Mg) cells, and patient-derived tumors. Both non-cancerous and cancerous mammary cells express connexins 26 and 43 using immunofluorescence; however, the level of expression is significantly different in quantitative analysis using western blot in which connexin 43 in both CMT12 and CMT27 is significantly decreased compared to CMEC. Furthermore, a decrease of gap junction capacity in CMT12 and CMT27 was observed compared to CMEC. Immunostaining of CMT27-xenograft tumors revealed positive Cx26 and negative Cx43 expression. Similarly, immunostaining of spontaneous canine mammary tumors revealed that Cx26 is present in all tumors while Cx43 is present in 25% of tumors. Overall, the study provides for the first time that a differential pattern of connexin expression exists between non-cancerous and cancerous mammary cells in dogs. This study will pave the path for further in vitro work of connexins in comparative canine models and possibly allow for novel therapeutics to be developed. |
format | Online Article Text |
id | pubmed-6958330 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-69583302020-01-23 Connexin 26 and Connexin 43 in Canine Mammary Carcinoma Luu, Savannah Bell, Cynthia Schneider, Sarah Nguyen, Thu Annelise Vet Sci Article Incidence of canine mammary carcinoma is two times higher than the rate of human breast cancer. Mammary tumors are the most common type of cancer in intact female dogs and account for about half of all neoplasms in these dogs. Well-established models of breast cancer have shown that neoplastic cells often have a loss of intercellular communication, particularly gap junction proteins. Thus, the objective of this study is to explore the aspect of gap junction intercellular communication in canine mammary carcinoma, non-cancerous (CMEC) and cancerous (CMT12, CMT27, and CF41.Mg) cells, and patient-derived tumors. Both non-cancerous and cancerous mammary cells express connexins 26 and 43 using immunofluorescence; however, the level of expression is significantly different in quantitative analysis using western blot in which connexin 43 in both CMT12 and CMT27 is significantly decreased compared to CMEC. Furthermore, a decrease of gap junction capacity in CMT12 and CMT27 was observed compared to CMEC. Immunostaining of CMT27-xenograft tumors revealed positive Cx26 and negative Cx43 expression. Similarly, immunostaining of spontaneous canine mammary tumors revealed that Cx26 is present in all tumors while Cx43 is present in 25% of tumors. Overall, the study provides for the first time that a differential pattern of connexin expression exists between non-cancerous and cancerous mammary cells in dogs. This study will pave the path for further in vitro work of connexins in comparative canine models and possibly allow for novel therapeutics to be developed. MDPI 2019-12-09 /pmc/articles/PMC6958330/ /pubmed/31818036 http://dx.doi.org/10.3390/vetsci6040101 Text en © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Luu, Savannah Bell, Cynthia Schneider, Sarah Nguyen, Thu Annelise Connexin 26 and Connexin 43 in Canine Mammary Carcinoma |
title | Connexin 26 and Connexin 43 in Canine Mammary Carcinoma |
title_full | Connexin 26 and Connexin 43 in Canine Mammary Carcinoma |
title_fullStr | Connexin 26 and Connexin 43 in Canine Mammary Carcinoma |
title_full_unstemmed | Connexin 26 and Connexin 43 in Canine Mammary Carcinoma |
title_short | Connexin 26 and Connexin 43 in Canine Mammary Carcinoma |
title_sort | connexin 26 and connexin 43 in canine mammary carcinoma |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6958330/ https://www.ncbi.nlm.nih.gov/pubmed/31818036 http://dx.doi.org/10.3390/vetsci6040101 |
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