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Effect of norbixin-based poly(hydroxybutyrate) membranes on the tendon repair process after tenotomy in rats

PURPOSE: To determine the efficacy of norbixin-based poly(hydroxybutyrate) (PHB) membranes for Achilles tendon repair. METHODS: Thirty rats were submitted to total tenotomy surgery of the right Achilles tendon and divided into two groups (control and membrane; n = 15 each), which were further subdiv...

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Detalles Bibliográficos
Autores principales: Nascimento, Lízia Daniela e Silva, Nicolau, Renata Amadei, Maia, Antônio Luiz Martins, Santos, José Zilton Lima Verde, Fonseca, Khetyma Moreira, Ferreira, Danniel Cabral Leão, de Sousa, Rayssilane Cardoso, Viana, Vicente Galber Freitas, Carvalho, Luiz Fernando Meneses, Figueredo-Silva, José
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6958576/
https://www.ncbi.nlm.nih.gov/pubmed/31939594
http://dx.doi.org/10.1590/s0102-865020190110000001
Descripción
Sumario:PURPOSE: To determine the efficacy of norbixin-based poly(hydroxybutyrate) (PHB) membranes for Achilles tendon repair. METHODS: Thirty rats were submitted to total tenotomy surgery of the right Achilles tendon and divided into two groups (control and membrane; n = 15 each), which were further subdivided into three subgroups (days 7, 14, and 21; n = 5 each). Samples were analyzed histologically. RESULTS: Histological analysis showed a significant reduction in inflammatory infiltrates on days 7, 14 (p < 0.0001 for both), and 21 (p = 0.0004) in the membrane group compared to that in the control group. There was also a significant decrease in the number of fibroblasts in the control group on days 7, 14 (p < 0.0001), and 21 (p = 0.0032). Further, an increase in type I collagen deposition was observed in the membrane group compared to that in the control group on days 7 (p = 0.0133) and 14 (p = 0.0107). CONCLUSION: Treatment with norbixin-based PHB membranes reduces the inflammatory response, increases fibroblast proliferation, and improves collagen production in the tendon repair region, especially between days 7 and 14.