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Transcriptome-wide map of m(6)A circRNAs identified in a rat model of hypoxia mediated pulmonary hypertension

BACKGROUND: Hypoxia mediated pulmonary hypertension (HPH) is a lethal disease and lacks effective therapy. CircRNAs play significant roles in physiological process. Recently, circRNAs are found to be m(6)A-modified. The abundance of circRNAs was influenced by m(6)A. Furthermore, the significance of...

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Autores principales: Su, Hua, Wang, Guowen, Wu, Lingfang, Ma, Xiuqing, Ying, Kejing, Zhang, Ruifeng
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6958941/
https://www.ncbi.nlm.nih.gov/pubmed/31931709
http://dx.doi.org/10.1186/s12864-020-6462-y
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author Su, Hua
Wang, Guowen
Wu, Lingfang
Ma, Xiuqing
Ying, Kejing
Zhang, Ruifeng
author_facet Su, Hua
Wang, Guowen
Wu, Lingfang
Ma, Xiuqing
Ying, Kejing
Zhang, Ruifeng
author_sort Su, Hua
collection PubMed
description BACKGROUND: Hypoxia mediated pulmonary hypertension (HPH) is a lethal disease and lacks effective therapy. CircRNAs play significant roles in physiological process. Recently, circRNAs are found to be m(6)A-modified. The abundance of circRNAs was influenced by m(6)A. Furthermore, the significance of m(6)A circRNAs has not been elucidated in HPH yet. Here we aim to investigate the transcriptome-wide map of m(6)A circRNAs in HPH. RESULTS: Differentially expressed m(6)A abundance was detected in lungs of HPH rats. M(6)A abundance in circRNAs was significantly reduced in hypoxia in vitro. M(6)A circRNAs were mainly from protein-coding genes spanned single exons in control and HPH groups. Moreover, m(6)A influenced the circRNA–miRNA–mRNA co-expression network in hypoxia. M(6)A circXpo6 and m(6)A circTmtc3 were firstly identified to be downregulated in HPH. CONCLUSION: Our study firstly identified the transcriptome-wide map of m(6)A circRNAs in HPH. M(6)A can influence circRNA–miRNA–mRNA network. Furthermore, we firstly identified two HPH-associated m(6)A circRNAs: circXpo6 and circTmtc3. However, the clinical significance of m(6)A circRNAs for HPH should be further validated.
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spelling pubmed-69589412020-01-17 Transcriptome-wide map of m(6)A circRNAs identified in a rat model of hypoxia mediated pulmonary hypertension Su, Hua Wang, Guowen Wu, Lingfang Ma, Xiuqing Ying, Kejing Zhang, Ruifeng BMC Genomics Research Article BACKGROUND: Hypoxia mediated pulmonary hypertension (HPH) is a lethal disease and lacks effective therapy. CircRNAs play significant roles in physiological process. Recently, circRNAs are found to be m(6)A-modified. The abundance of circRNAs was influenced by m(6)A. Furthermore, the significance of m(6)A circRNAs has not been elucidated in HPH yet. Here we aim to investigate the transcriptome-wide map of m(6)A circRNAs in HPH. RESULTS: Differentially expressed m(6)A abundance was detected in lungs of HPH rats. M(6)A abundance in circRNAs was significantly reduced in hypoxia in vitro. M(6)A circRNAs were mainly from protein-coding genes spanned single exons in control and HPH groups. Moreover, m(6)A influenced the circRNA–miRNA–mRNA co-expression network in hypoxia. M(6)A circXpo6 and m(6)A circTmtc3 were firstly identified to be downregulated in HPH. CONCLUSION: Our study firstly identified the transcriptome-wide map of m(6)A circRNAs in HPH. M(6)A can influence circRNA–miRNA–mRNA network. Furthermore, we firstly identified two HPH-associated m(6)A circRNAs: circXpo6 and circTmtc3. However, the clinical significance of m(6)A circRNAs for HPH should be further validated. BioMed Central 2020-01-13 /pmc/articles/PMC6958941/ /pubmed/31931709 http://dx.doi.org/10.1186/s12864-020-6462-y Text en © The Author(s). 2020 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
spellingShingle Research Article
Su, Hua
Wang, Guowen
Wu, Lingfang
Ma, Xiuqing
Ying, Kejing
Zhang, Ruifeng
Transcriptome-wide map of m(6)A circRNAs identified in a rat model of hypoxia mediated pulmonary hypertension
title Transcriptome-wide map of m(6)A circRNAs identified in a rat model of hypoxia mediated pulmonary hypertension
title_full Transcriptome-wide map of m(6)A circRNAs identified in a rat model of hypoxia mediated pulmonary hypertension
title_fullStr Transcriptome-wide map of m(6)A circRNAs identified in a rat model of hypoxia mediated pulmonary hypertension
title_full_unstemmed Transcriptome-wide map of m(6)A circRNAs identified in a rat model of hypoxia mediated pulmonary hypertension
title_short Transcriptome-wide map of m(6)A circRNAs identified in a rat model of hypoxia mediated pulmonary hypertension
title_sort transcriptome-wide map of m(6)a circrnas identified in a rat model of hypoxia mediated pulmonary hypertension
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6958941/
https://www.ncbi.nlm.nih.gov/pubmed/31931709
http://dx.doi.org/10.1186/s12864-020-6462-y
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