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Effect of ketotifen fumarate on experimental autoimmune orchitis and torsion of the spermatic cord
The aim of this work was to study effects of ketotifen fumarate (KF) on prevention of tissue damage in testes of rats with experimental autoimmune orchitis (EAO) and on the contralateral testis in a model of prolonged testicular cord torsion (TCT). Rats with EAO or TCT were injected intraperitoneall...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Wolters Kluwer - Medknow
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6958983/ https://www.ncbi.nlm.nih.gov/pubmed/31115365 http://dx.doi.org/10.4103/aja.aja_30_19 |
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author | Moreno, Diego Sobarzo, Cristian M Lustig, Livia Rodríguez Peña, Marcelo G Guazzone, Vanesa Anabella |
author_facet | Moreno, Diego Sobarzo, Cristian M Lustig, Livia Rodríguez Peña, Marcelo G Guazzone, Vanesa Anabella |
author_sort | Moreno, Diego |
collection | PubMed |
description | The aim of this work was to study effects of ketotifen fumarate (KF) on prevention of tissue damage in testes of rats with experimental autoimmune orchitis (EAO) and on the contralateral testis in a model of prolonged testicular cord torsion (TCT). Rats with EAO or TCT were injected intraperitoneally once daily with KF or saline solution (vehicle group). Incidence and severity of testicular damage were evaluated by histopathology using an EAO score or a Johnsen score. Mast cells (MC) were identified by histochemistry and quantified. In EAO model, KF significantly reduced severity of histopathological testicular damage compared to rats in the vehicle group. KF also reduced the number of testicular MC compared to vehicle group. Similarly, in TCT model, multifocal damage of the contralateral testis was observed 30 days after testicular torsion characterized by sloughing of the germinal epithelium, seminiferous tubule atrophy, and interstitial edema. Focal signs of inflammation and fibrosis of seminiferous tubular walls were also observed. In contrast, sections of contralateral testis of rats injected with KF and killed 30 days after surgery showed normal histological features. A significant decrease in the number of MC was observed in rats treated with KF compared to untreated animals. In conclusion, we demonstrated that treatment with KF reduced testicular inflammatory process and MC infiltrates in both EAO and TCT models. The results suggest a promising treatment for infertile male patients with testicular pathologies associated with inflammation and germ cell loss. |
format | Online Article Text |
id | pubmed-6958983 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | Wolters Kluwer - Medknow |
record_format | MEDLINE/PubMed |
spelling | pubmed-69589832020-01-16 Effect of ketotifen fumarate on experimental autoimmune orchitis and torsion of the spermatic cord Moreno, Diego Sobarzo, Cristian M Lustig, Livia Rodríguez Peña, Marcelo G Guazzone, Vanesa Anabella Asian J Androl Original Article The aim of this work was to study effects of ketotifen fumarate (KF) on prevention of tissue damage in testes of rats with experimental autoimmune orchitis (EAO) and on the contralateral testis in a model of prolonged testicular cord torsion (TCT). Rats with EAO or TCT were injected intraperitoneally once daily with KF or saline solution (vehicle group). Incidence and severity of testicular damage were evaluated by histopathology using an EAO score or a Johnsen score. Mast cells (MC) were identified by histochemistry and quantified. In EAO model, KF significantly reduced severity of histopathological testicular damage compared to rats in the vehicle group. KF also reduced the number of testicular MC compared to vehicle group. Similarly, in TCT model, multifocal damage of the contralateral testis was observed 30 days after testicular torsion characterized by sloughing of the germinal epithelium, seminiferous tubule atrophy, and interstitial edema. Focal signs of inflammation and fibrosis of seminiferous tubular walls were also observed. In contrast, sections of contralateral testis of rats injected with KF and killed 30 days after surgery showed normal histological features. A significant decrease in the number of MC was observed in rats treated with KF compared to untreated animals. In conclusion, we demonstrated that treatment with KF reduced testicular inflammatory process and MC infiltrates in both EAO and TCT models. The results suggest a promising treatment for infertile male patients with testicular pathologies associated with inflammation and germ cell loss. Wolters Kluwer - Medknow 2019-05-17 /pmc/articles/PMC6958983/ /pubmed/31115365 http://dx.doi.org/10.4103/aja.aja_30_19 Text en Copyright: © The Author(s)(2019) http://creativecommons.org/licenses/by-nc-sa/4.0 This is an open access journal, and articles are distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike 4.0 License, which allows others to remix, tweak, and build upon the work non-commercially, as long as appropriate credit is given and the new creations are licensed under the identical terms. |
spellingShingle | Original Article Moreno, Diego Sobarzo, Cristian M Lustig, Livia Rodríguez Peña, Marcelo G Guazzone, Vanesa Anabella Effect of ketotifen fumarate on experimental autoimmune orchitis and torsion of the spermatic cord |
title | Effect of ketotifen fumarate on experimental autoimmune orchitis and torsion of the spermatic cord |
title_full | Effect of ketotifen fumarate on experimental autoimmune orchitis and torsion of the spermatic cord |
title_fullStr | Effect of ketotifen fumarate on experimental autoimmune orchitis and torsion of the spermatic cord |
title_full_unstemmed | Effect of ketotifen fumarate on experimental autoimmune orchitis and torsion of the spermatic cord |
title_short | Effect of ketotifen fumarate on experimental autoimmune orchitis and torsion of the spermatic cord |
title_sort | effect of ketotifen fumarate on experimental autoimmune orchitis and torsion of the spermatic cord |
topic | Original Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6958983/ https://www.ncbi.nlm.nih.gov/pubmed/31115365 http://dx.doi.org/10.4103/aja.aja_30_19 |
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