Rapid Affinity Maturation of Novel Anti-PD-L1 Antibodies by a Fast Drop of the Antigen Concentration and FACS Selection of Yeast Libraries

The affinity engineering is a key step to increase the efficacy of therapeutic monoclonal antibodies and yeast surface display is the most widely used and powerful affinity maturation approach, achieving picomolar binding affinities. In this study, we provide an optimization of the yeast surface dis...

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Detalles Bibliográficos
Autores principales: Cembrola, Biancamaria, Ruzza, Valentino, Troise, Fulvia, Esposito, Maria Luisa, Sasso, Emanuele, Cafaro, Valeria, Passariello, Margherita, Visconte, Feliciano, Raia, Maddalena, Del Vecchio, Luigi, D'Alise, Anna Morena, Cortese, Riccardo, Scarselli, Elisa, Zambrano, Nicola, De Lorenzo, Claudia, Nicosia, Alfredo
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi 2019
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6959147/
https://www.ncbi.nlm.nih.gov/pubmed/31976323
http://dx.doi.org/10.1155/2019/6051870

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