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Flow cytometric analysis of neutrophil myeloperoxidase expression in peripheral blood for ruling out myelodysplastic syndromes: a diagnostic accuracy study

Suspicion of myelodysplastic syndromes (MDS) is one of the commonest reasons for bone marrow aspirate in elderly patients presenting with persistent peripheral blood (PB) cytopenia of unclear etiology. A PB assay that accurately rules out MDS would have major benefits. The diagnostic accuracy of the...

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Autores principales: Raskovalova, Tatiana, Berger, Marc G., Jacob, Marie-Christine, Park, Sophie, Campos, Lydia, Aanei, Carmen Mariana, Kasprzak, Julie, Pereira, Bruno, Labarère, José, Cesbron, Jean-Yves, Veyrat-Masson, Richard
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Ferrata Storti Foundation 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6959174/
https://www.ncbi.nlm.nih.gov/pubmed/31004030
http://dx.doi.org/10.3324/haematol.2018.202275
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author Raskovalova, Tatiana
Berger, Marc G.
Jacob, Marie-Christine
Park, Sophie
Campos, Lydia
Aanei, Carmen Mariana
Kasprzak, Julie
Pereira, Bruno
Labarère, José
Cesbron, Jean-Yves
Veyrat-Masson, Richard
author_facet Raskovalova, Tatiana
Berger, Marc G.
Jacob, Marie-Christine
Park, Sophie
Campos, Lydia
Aanei, Carmen Mariana
Kasprzak, Julie
Pereira, Bruno
Labarère, José
Cesbron, Jean-Yves
Veyrat-Masson, Richard
author_sort Raskovalova, Tatiana
collection PubMed
description Suspicion of myelodysplastic syndromes (MDS) is one of the commonest reasons for bone marrow aspirate in elderly patients presenting with persistent peripheral blood (PB) cytopenia of unclear etiology. A PB assay that accurately rules out MDS would have major benefits. The diagnostic accuracy of the intra-individual robust coefficient of variation (RCV) for neutrophil myeloperoxidase (MPO) expression measured by flow cytometric analysis in PB was evaluated in a retrospective derivation study (44 MDS cases and 44 controls) and a prospective validation study (68 consecutive patients with suspected MDS). Compared with controls, MDS cases had higher median RCV values for neutrophil MPO expression (40.2% vs. 30.9%; P<0.001). The area under the receiver operating characteristic curve estimates were 0.94 [95% confidence interval (CI): 0.86-0.97] and 0.87 (95%CI: 0.76-0.94) in the derivation and validation studies, respectively. A RCV lower than 30% ruled out MDS with 100% sensitivity (95%CI: 78-100%) and 100% negative predictive value (95%CI: 83-100%) in the prospective validation study. Neutrophil MPO expression measured by flow cytometric analysis in PB might obviate the need for invasive bone marrow aspirate and biopsy for up to 29% of patients with suspected MDS.
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spelling pubmed-69591742020-01-22 Flow cytometric analysis of neutrophil myeloperoxidase expression in peripheral blood for ruling out myelodysplastic syndromes: a diagnostic accuracy study Raskovalova, Tatiana Berger, Marc G. Jacob, Marie-Christine Park, Sophie Campos, Lydia Aanei, Carmen Mariana Kasprzak, Julie Pereira, Bruno Labarère, José Cesbron, Jean-Yves Veyrat-Masson, Richard Haematologica Article Suspicion of myelodysplastic syndromes (MDS) is one of the commonest reasons for bone marrow aspirate in elderly patients presenting with persistent peripheral blood (PB) cytopenia of unclear etiology. A PB assay that accurately rules out MDS would have major benefits. The diagnostic accuracy of the intra-individual robust coefficient of variation (RCV) for neutrophil myeloperoxidase (MPO) expression measured by flow cytometric analysis in PB was evaluated in a retrospective derivation study (44 MDS cases and 44 controls) and a prospective validation study (68 consecutive patients with suspected MDS). Compared with controls, MDS cases had higher median RCV values for neutrophil MPO expression (40.2% vs. 30.9%; P<0.001). The area under the receiver operating characteristic curve estimates were 0.94 [95% confidence interval (CI): 0.86-0.97] and 0.87 (95%CI: 0.76-0.94) in the derivation and validation studies, respectively. A RCV lower than 30% ruled out MDS with 100% sensitivity (95%CI: 78-100%) and 100% negative predictive value (95%CI: 83-100%) in the prospective validation study. Neutrophil MPO expression measured by flow cytometric analysis in PB might obviate the need for invasive bone marrow aspirate and biopsy for up to 29% of patients with suspected MDS. Ferrata Storti Foundation 2019-12 /pmc/articles/PMC6959174/ /pubmed/31004030 http://dx.doi.org/10.3324/haematol.2018.202275 Text en Copyright© 2019 Ferrata Storti Foundation Material published in Haematologica is covered by copyright. All rights are reserved to the Ferrata Storti Foundation. Use of published material is allowed under the following terms and conditions: https://creativecommons.org/licenses/by-nc/4.0/legalcode. Copies of published material are allowed for personal or internal use. Sharing published material for non-commercial purposes is subject to the following conditions: https://creativecommons.org/licenses/by-nc/4.0/legalcode, sect. 3. Reproducing and sharing published material for commercial purposes is not allowed without permission in writing from the publisher.
spellingShingle Article
Raskovalova, Tatiana
Berger, Marc G.
Jacob, Marie-Christine
Park, Sophie
Campos, Lydia
Aanei, Carmen Mariana
Kasprzak, Julie
Pereira, Bruno
Labarère, José
Cesbron, Jean-Yves
Veyrat-Masson, Richard
Flow cytometric analysis of neutrophil myeloperoxidase expression in peripheral blood for ruling out myelodysplastic syndromes: a diagnostic accuracy study
title Flow cytometric analysis of neutrophil myeloperoxidase expression in peripheral blood for ruling out myelodysplastic syndromes: a diagnostic accuracy study
title_full Flow cytometric analysis of neutrophil myeloperoxidase expression in peripheral blood for ruling out myelodysplastic syndromes: a diagnostic accuracy study
title_fullStr Flow cytometric analysis of neutrophil myeloperoxidase expression in peripheral blood for ruling out myelodysplastic syndromes: a diagnostic accuracy study
title_full_unstemmed Flow cytometric analysis of neutrophil myeloperoxidase expression in peripheral blood for ruling out myelodysplastic syndromes: a diagnostic accuracy study
title_short Flow cytometric analysis of neutrophil myeloperoxidase expression in peripheral blood for ruling out myelodysplastic syndromes: a diagnostic accuracy study
title_sort flow cytometric analysis of neutrophil myeloperoxidase expression in peripheral blood for ruling out myelodysplastic syndromes: a diagnostic accuracy study
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6959174/
https://www.ncbi.nlm.nih.gov/pubmed/31004030
http://dx.doi.org/10.3324/haematol.2018.202275
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