Sensitive detection of a bacterial pathogen using allosteric probe-initiated catalysis and CRISPR-Cas13a amplification reaction

The ability to detect low numbers of microbial cells in food and clinical samples is highly valuable but remains a challenge. Here we present a detection system (called ‘APC-Cas’) that can detect very low numbers of a bacterial pathogen without isolation, using a three-stage amplification to generat...

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Detalles Bibliográficos
Autores principales: Shen, Jinjin, Zhou, Xiaoming, Shan, Yuanyue, Yue, Huahua, Huang, Ru, Hu, Jiaming, Xing, Da
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2020
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6959245/
https://www.ncbi.nlm.nih.gov/pubmed/31937772
http://dx.doi.org/10.1038/s41467-019-14135-9

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6959245/
https://www.ncbi.nlm.nih.gov/pubmed/31937772
http://dx.doi.org/10.1038/s41467-019-14135-9

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