Cargando…
Oxidised Met(147) of human serum albumin is a biomarker of oxidative stress, reflecting glycaemic fluctuations and hypoglycaemia in diabetes
Oxidative stress has been linked to a number of chronic diseases, and this has aroused interest in the identification of clinical biomarkers that can accurately assess its severity. We used liquid chromatography-high resolution mass spectrometry (LC-MS) to show that oxidised and non-oxidised Met res...
Autores principales: | , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group UK
2020
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6959251/ https://www.ncbi.nlm.nih.gov/pubmed/31937809 http://dx.doi.org/10.1038/s41598-019-57095-2 |
_version_ | 1783487556010115072 |
---|---|
author | Momozono, Akari Kodera, Yoshio Sasaki, Sayaka Nakagawa, Yuzuru Konno, Ryo Shichiri, Masayoshi |
author_facet | Momozono, Akari Kodera, Yoshio Sasaki, Sayaka Nakagawa, Yuzuru Konno, Ryo Shichiri, Masayoshi |
author_sort | Momozono, Akari |
collection | PubMed |
description | Oxidative stress has been linked to a number of chronic diseases, and this has aroused interest in the identification of clinical biomarkers that can accurately assess its severity. We used liquid chromatography-high resolution mass spectrometry (LC-MS) to show that oxidised and non-oxidised Met residues at position 147 of human serum albumin (Met(147)) can be accurately and reproducibly quantified with stable isotope-labelled peptides. Met(147) oxidation was significantly higher in patients with diabetes than in controls. Least square multivariate analysis revealed that glycated haemoglobin (HbA(1c)) and glycated albumin (GA) did not significantly influence Met(147) oxidation, but the GA/HbA(1c) ratio, which reflects glycaemic excursions, independently affected Met(147) oxidation status. Continuous glucose monitoring revealed that Met(147) oxidation strongly correlates with the standard deviation of sensor glucose concentrations and the time spent with hypoglycaemia or hyperglycaemia each day. Thus, glycaemic variability and hypoglycaemia in diabetes may be associated with greater oxidation of Met(147). Renal function, high-density lipoprotein-cholesterol and serum bilirubin were also associated with the oxidation status of Met(147). In conclusion, the quantification of oxidised and non-oxidised Met(147) in serum albumin using our LC-MS methodology could be used to assess the degree of intravascular oxidative stress induced by hypoglycaemia and glycaemic fluctuations in diabetes. |
format | Online Article Text |
id | pubmed-6959251 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | Nature Publishing Group UK |
record_format | MEDLINE/PubMed |
spelling | pubmed-69592512020-01-16 Oxidised Met(147) of human serum albumin is a biomarker of oxidative stress, reflecting glycaemic fluctuations and hypoglycaemia in diabetes Momozono, Akari Kodera, Yoshio Sasaki, Sayaka Nakagawa, Yuzuru Konno, Ryo Shichiri, Masayoshi Sci Rep Article Oxidative stress has been linked to a number of chronic diseases, and this has aroused interest in the identification of clinical biomarkers that can accurately assess its severity. We used liquid chromatography-high resolution mass spectrometry (LC-MS) to show that oxidised and non-oxidised Met residues at position 147 of human serum albumin (Met(147)) can be accurately and reproducibly quantified with stable isotope-labelled peptides. Met(147) oxidation was significantly higher in patients with diabetes than in controls. Least square multivariate analysis revealed that glycated haemoglobin (HbA(1c)) and glycated albumin (GA) did not significantly influence Met(147) oxidation, but the GA/HbA(1c) ratio, which reflects glycaemic excursions, independently affected Met(147) oxidation status. Continuous glucose monitoring revealed that Met(147) oxidation strongly correlates with the standard deviation of sensor glucose concentrations and the time spent with hypoglycaemia or hyperglycaemia each day. Thus, glycaemic variability and hypoglycaemia in diabetes may be associated with greater oxidation of Met(147). Renal function, high-density lipoprotein-cholesterol and serum bilirubin were also associated with the oxidation status of Met(147). In conclusion, the quantification of oxidised and non-oxidised Met(147) in serum albumin using our LC-MS methodology could be used to assess the degree of intravascular oxidative stress induced by hypoglycaemia and glycaemic fluctuations in diabetes. Nature Publishing Group UK 2020-01-14 /pmc/articles/PMC6959251/ /pubmed/31937809 http://dx.doi.org/10.1038/s41598-019-57095-2 Text en © The Author(s) 2020 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/. |
spellingShingle | Article Momozono, Akari Kodera, Yoshio Sasaki, Sayaka Nakagawa, Yuzuru Konno, Ryo Shichiri, Masayoshi Oxidised Met(147) of human serum albumin is a biomarker of oxidative stress, reflecting glycaemic fluctuations and hypoglycaemia in diabetes |
title | Oxidised Met(147) of human serum albumin is a biomarker of oxidative stress, reflecting glycaemic fluctuations and hypoglycaemia in diabetes |
title_full | Oxidised Met(147) of human serum albumin is a biomarker of oxidative stress, reflecting glycaemic fluctuations and hypoglycaemia in diabetes |
title_fullStr | Oxidised Met(147) of human serum albumin is a biomarker of oxidative stress, reflecting glycaemic fluctuations and hypoglycaemia in diabetes |
title_full_unstemmed | Oxidised Met(147) of human serum albumin is a biomarker of oxidative stress, reflecting glycaemic fluctuations and hypoglycaemia in diabetes |
title_short | Oxidised Met(147) of human serum albumin is a biomarker of oxidative stress, reflecting glycaemic fluctuations and hypoglycaemia in diabetes |
title_sort | oxidised met(147) of human serum albumin is a biomarker of oxidative stress, reflecting glycaemic fluctuations and hypoglycaemia in diabetes |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6959251/ https://www.ncbi.nlm.nih.gov/pubmed/31937809 http://dx.doi.org/10.1038/s41598-019-57095-2 |
work_keys_str_mv | AT momozonoakari oxidisedmet147ofhumanserumalbuminisabiomarkerofoxidativestressreflectingglycaemicfluctuationsandhypoglycaemiaindiabetes AT koderayoshio oxidisedmet147ofhumanserumalbuminisabiomarkerofoxidativestressreflectingglycaemicfluctuationsandhypoglycaemiaindiabetes AT sasakisayaka oxidisedmet147ofhumanserumalbuminisabiomarkerofoxidativestressreflectingglycaemicfluctuationsandhypoglycaemiaindiabetes AT nakagawayuzuru oxidisedmet147ofhumanserumalbuminisabiomarkerofoxidativestressreflectingglycaemicfluctuationsandhypoglycaemiaindiabetes AT konnoryo oxidisedmet147ofhumanserumalbuminisabiomarkerofoxidativestressreflectingglycaemicfluctuationsandhypoglycaemiaindiabetes AT shichirimasayoshi oxidisedmet147ofhumanserumalbuminisabiomarkerofoxidativestressreflectingglycaemicfluctuationsandhypoglycaemiaindiabetes |