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Stability of extracts from pollens of allergenic importance in Korea
BACKGROUND/AIMS: Accurate diagnosis and the effects of allergen-specific immunotherapy for pollinosis are greatly dependent on the potency and stability of the extract. This study aimed to examine factors, such as temperature and storage buffer composition, that affect the stability of allergen extr...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
The Korean Association of Internal Medicine
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6960046/ https://www.ncbi.nlm.nih.gov/pubmed/31534112 http://dx.doi.org/10.3904/kjim.2018.211 |
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author | Jeong, Kyoung Yong Yuk, Ji Eun Lee, Jongsun Jang, Seok Woo Park, Kyung Hee Lee, Jae-Hyun Park, Jung-Won |
author_facet | Jeong, Kyoung Yong Yuk, Ji Eun Lee, Jongsun Jang, Seok Woo Park, Kyung Hee Lee, Jae-Hyun Park, Jung-Won |
author_sort | Jeong, Kyoung Yong |
collection | PubMed |
description | BACKGROUND/AIMS: Accurate diagnosis and the effects of allergen-specific immunotherapy for pollinosis are greatly dependent on the potency and stability of the extract. This study aimed to examine factors, such as temperature and storage buffer composition, that affect the stability of allergen extracts from pollens of allergenic importance in Korea. METHODS: We prepared four pollen allergen extracts from ragweed, mugwort, Japanese hop, and sawtooth oak, which are the most important causes of seasonal rhinitis in Korea. Changes of protein and major allergen concentration were measured over 1 year by Bradford assay, two-site enzyme-linked immunosorbent assay, and sodium dodecyl sulfate-polyacrylamide gel electrophoresis after reconstitution of the lyophilized allergen extract in various buffers and stored at room temperature (RT, 18°C to 26°C) or refrigerated (4°C). RESULTS: More than 90% of the original protein concentration in all four extracts examined was detected over 1 year when 50% glycerol was added and refrigerated, whereas 57.9% to 94.5% remained in the extracts at RT. The addition of 50% glycerol to the storage buffer was found to prevent protein degradation at RT. Amb a 1, a major allergen of ragweed, was almost completely degraded in 9 weeks at RT when reconstituted in a buffer without 50% glycerol. However, 55.6% to 92.8% of Amb a 1 content was detected after 1 year of incubation at 4°C in all buffer conditions except 0.3% phenol. CONCLUSIONS: Addition of 50% glycerol as well as refrigeration was found to be important in increasing the shelf-life of allergen extracts from pollens of allergenic importance. |
format | Online Article Text |
id | pubmed-6960046 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | The Korean Association of Internal Medicine |
record_format | MEDLINE/PubMed |
spelling | pubmed-69600462020-01-22 Stability of extracts from pollens of allergenic importance in Korea Jeong, Kyoung Yong Yuk, Ji Eun Lee, Jongsun Jang, Seok Woo Park, Kyung Hee Lee, Jae-Hyun Park, Jung-Won Korean J Intern Med Original Article BACKGROUND/AIMS: Accurate diagnosis and the effects of allergen-specific immunotherapy for pollinosis are greatly dependent on the potency and stability of the extract. This study aimed to examine factors, such as temperature and storage buffer composition, that affect the stability of allergen extracts from pollens of allergenic importance in Korea. METHODS: We prepared four pollen allergen extracts from ragweed, mugwort, Japanese hop, and sawtooth oak, which are the most important causes of seasonal rhinitis in Korea. Changes of protein and major allergen concentration were measured over 1 year by Bradford assay, two-site enzyme-linked immunosorbent assay, and sodium dodecyl sulfate-polyacrylamide gel electrophoresis after reconstitution of the lyophilized allergen extract in various buffers and stored at room temperature (RT, 18°C to 26°C) or refrigerated (4°C). RESULTS: More than 90% of the original protein concentration in all four extracts examined was detected over 1 year when 50% glycerol was added and refrigerated, whereas 57.9% to 94.5% remained in the extracts at RT. The addition of 50% glycerol to the storage buffer was found to prevent protein degradation at RT. Amb a 1, a major allergen of ragweed, was almost completely degraded in 9 weeks at RT when reconstituted in a buffer without 50% glycerol. However, 55.6% to 92.8% of Amb a 1 content was detected after 1 year of incubation at 4°C in all buffer conditions except 0.3% phenol. CONCLUSIONS: Addition of 50% glycerol as well as refrigeration was found to be important in increasing the shelf-life of allergen extracts from pollens of allergenic importance. The Korean Association of Internal Medicine 2020-01 2019-09-20 /pmc/articles/PMC6960046/ /pubmed/31534112 http://dx.doi.org/10.3904/kjim.2018.211 Text en Copyright © 2020 The Korean Association of Internal Medicine This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0/) which permits unrestricted noncommercial use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Original Article Jeong, Kyoung Yong Yuk, Ji Eun Lee, Jongsun Jang, Seok Woo Park, Kyung Hee Lee, Jae-Hyun Park, Jung-Won Stability of extracts from pollens of allergenic importance in Korea |
title | Stability of extracts from pollens of allergenic importance in Korea |
title_full | Stability of extracts from pollens of allergenic importance in Korea |
title_fullStr | Stability of extracts from pollens of allergenic importance in Korea |
title_full_unstemmed | Stability of extracts from pollens of allergenic importance in Korea |
title_short | Stability of extracts from pollens of allergenic importance in Korea |
title_sort | stability of extracts from pollens of allergenic importance in korea |
topic | Original Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6960046/ https://www.ncbi.nlm.nih.gov/pubmed/31534112 http://dx.doi.org/10.3904/kjim.2018.211 |
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