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Transcription and Activity of Digestive Enzymes of Nezara viridula Maintained on Different Plant Diets
Nezara viridula is a polyphagous stink bug that feeds on crops of economic importance such as corn, soybean and cotton. To increase understanding of the ability of this pest insect to feed on such diverse cropping systems, we analyzed the impact of an exclusive diet of corn or green bean on the enzy...
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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Frontiers Media S.A.
2020
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6960134/ https://www.ncbi.nlm.nih.gov/pubmed/31969835 http://dx.doi.org/10.3389/fphys.2019.01553 |
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author | Cantón, Pablo Emiliano Bonning, Bryony C. |
author_facet | Cantón, Pablo Emiliano Bonning, Bryony C. |
author_sort | Cantón, Pablo Emiliano |
collection | PubMed |
description | Nezara viridula is a polyphagous stink bug that feeds on crops of economic importance such as corn, soybean and cotton. To increase understanding of the ability of this pest insect to feed on such diverse cropping systems, we analyzed the impact of an exclusive diet of corn or green bean on the enzymatic activity and transcriptomic profile of digestive enzymes. Growth rate and survival were reduced when insects were reared exclusively on green bean compared to corn. However, the overall protease and nuclease activity profiles were comparable between the two treatments. Distinct differences in inhibitor sensitivity and activity were seen in some cases, particularly for serine proteases in some regions of the midgut. The transcription profiles from N. viridula fed on corn versus green bean were distinct on principal component analysis of RNA-seq data. While specific transcripts differentially transcribed according to diet and across several tissues were identified, a large number of these transcripts remain unannotated. Further annotation for identification of these genes will be important for improved understanding of the remarkable polyphagy of N. viridula. |
format | Online Article Text |
id | pubmed-6960134 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-69601342020-01-22 Transcription and Activity of Digestive Enzymes of Nezara viridula Maintained on Different Plant Diets Cantón, Pablo Emiliano Bonning, Bryony C. Front Physiol Physiology Nezara viridula is a polyphagous stink bug that feeds on crops of economic importance such as corn, soybean and cotton. To increase understanding of the ability of this pest insect to feed on such diverse cropping systems, we analyzed the impact of an exclusive diet of corn or green bean on the enzymatic activity and transcriptomic profile of digestive enzymes. Growth rate and survival were reduced when insects were reared exclusively on green bean compared to corn. However, the overall protease and nuclease activity profiles were comparable between the two treatments. Distinct differences in inhibitor sensitivity and activity were seen in some cases, particularly for serine proteases in some regions of the midgut. The transcription profiles from N. viridula fed on corn versus green bean were distinct on principal component analysis of RNA-seq data. While specific transcripts differentially transcribed according to diet and across several tissues were identified, a large number of these transcripts remain unannotated. Further annotation for identification of these genes will be important for improved understanding of the remarkable polyphagy of N. viridula. Frontiers Media S.A. 2020-01-08 /pmc/articles/PMC6960134/ /pubmed/31969835 http://dx.doi.org/10.3389/fphys.2019.01553 Text en Copyright © 2020 Cantón and Bonning. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Physiology Cantón, Pablo Emiliano Bonning, Bryony C. Transcription and Activity of Digestive Enzymes of Nezara viridula Maintained on Different Plant Diets |
title | Transcription and Activity of Digestive Enzymes of Nezara viridula Maintained on Different Plant Diets |
title_full | Transcription and Activity of Digestive Enzymes of Nezara viridula Maintained on Different Plant Diets |
title_fullStr | Transcription and Activity of Digestive Enzymes of Nezara viridula Maintained on Different Plant Diets |
title_full_unstemmed | Transcription and Activity of Digestive Enzymes of Nezara viridula Maintained on Different Plant Diets |
title_short | Transcription and Activity of Digestive Enzymes of Nezara viridula Maintained on Different Plant Diets |
title_sort | transcription and activity of digestive enzymes of nezara viridula maintained on different plant diets |
topic | Physiology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6960134/ https://www.ncbi.nlm.nih.gov/pubmed/31969835 http://dx.doi.org/10.3389/fphys.2019.01553 |
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