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Application of Autofluorescence for Confocal Microscopy to Aid in Archaeoparasitological Analyses

Confocal laser scanning microscopy (CLSM) was used to examine archaeoparasitological specimens from coprolites associated with La Cueva de los Muertos Chiquitos (CMC) located near present-day Durango, Mexico. The eggs for 4 different types of parasites recovered from CMC coprolites were imaged using...

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Autores principales: Morrow, Johnica Jo, Elowsky, Christian
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Korean Society for Parasitology and Tropical Medicine 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6960251/
https://www.ncbi.nlm.nih.gov/pubmed/31914508
http://dx.doi.org/10.3347/kjp.2019.57.6.581
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author Morrow, Johnica Jo
Elowsky, Christian
author_facet Morrow, Johnica Jo
Elowsky, Christian
author_sort Morrow, Johnica Jo
collection PubMed
description Confocal laser scanning microscopy (CLSM) was used to examine archaeoparasitological specimens from coprolites associated with La Cueva de los Muertos Chiquitos (CMC) located near present-day Durango, Mexico. The eggs for 4 different types of parasites recovered from CMC coprolites were imaged using CLSM to assist with identification efforts. While some of the parasite eggs recovered from CMC coprolites were readily identified using standard light microscopy (LM), CLSM provided useful data for more challenging identifications by highlighting subtle morphological features and enhancing visualization of parasite egg anatomy. While other advanced microscopy techniques, such as scanning electron microscopy (SEM), may also detect cryptic identifying characters, CLSM is less destructive to the specimens. Utilizing CLSM allows for subsequent examinations, such as molecular analyses, that cannot be performed following SEM sample preparation and imaging. Furthermore, CLSM detects intrinsic autofluorescence molecules, making improved identification independent of resource and time-intensive protocols. These aspects of CLSM make it an excellent method for assisting in taxonomic identification and for acquiring more detailed images of archaeoparasitological specimens.
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spelling pubmed-69602512020-01-22 Application of Autofluorescence for Confocal Microscopy to Aid in Archaeoparasitological Analyses Morrow, Johnica Jo Elowsky, Christian Korean J Parasitol Original Article Confocal laser scanning microscopy (CLSM) was used to examine archaeoparasitological specimens from coprolites associated with La Cueva de los Muertos Chiquitos (CMC) located near present-day Durango, Mexico. The eggs for 4 different types of parasites recovered from CMC coprolites were imaged using CLSM to assist with identification efforts. While some of the parasite eggs recovered from CMC coprolites were readily identified using standard light microscopy (LM), CLSM provided useful data for more challenging identifications by highlighting subtle morphological features and enhancing visualization of parasite egg anatomy. While other advanced microscopy techniques, such as scanning electron microscopy (SEM), may also detect cryptic identifying characters, CLSM is less destructive to the specimens. Utilizing CLSM allows for subsequent examinations, such as molecular analyses, that cannot be performed following SEM sample preparation and imaging. Furthermore, CLSM detects intrinsic autofluorescence molecules, making improved identification independent of resource and time-intensive protocols. These aspects of CLSM make it an excellent method for assisting in taxonomic identification and for acquiring more detailed images of archaeoparasitological specimens. The Korean Society for Parasitology and Tropical Medicine 2019-12 2019-12-31 /pmc/articles/PMC6960251/ /pubmed/31914508 http://dx.doi.org/10.3347/kjp.2019.57.6.581 Text en Copyright © 2019 by The Korean Society for Parasitology and Tropical Medicine This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Original Article
Morrow, Johnica Jo
Elowsky, Christian
Application of Autofluorescence for Confocal Microscopy to Aid in Archaeoparasitological Analyses
title Application of Autofluorescence for Confocal Microscopy to Aid in Archaeoparasitological Analyses
title_full Application of Autofluorescence for Confocal Microscopy to Aid in Archaeoparasitological Analyses
title_fullStr Application of Autofluorescence for Confocal Microscopy to Aid in Archaeoparasitological Analyses
title_full_unstemmed Application of Autofluorescence for Confocal Microscopy to Aid in Archaeoparasitological Analyses
title_short Application of Autofluorescence for Confocal Microscopy to Aid in Archaeoparasitological Analyses
title_sort application of autofluorescence for confocal microscopy to aid in archaeoparasitological analyses
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6960251/
https://www.ncbi.nlm.nih.gov/pubmed/31914508
http://dx.doi.org/10.3347/kjp.2019.57.6.581
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