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PCB-Based Magnetometer as a Platform for Quantification of Lateral-Flow Assays
This work presents a proof-of-concept demonstration of a novel inductive transducer, the femtoMag, that can be integrated with a lateral-flow assay (LFA) to provide detection and quantification of molecular biomarkers. The femtoMag transducer is manufactured using a low-cost printed circuit board (P...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6960494/ https://www.ncbi.nlm.nih.gov/pubmed/31835468 http://dx.doi.org/10.3390/s19245433 |
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author | Khodadadi, Mohammad Chang, Long Trabuco, João R. C. Vu, Binh V. Kourentzi, Katerina Willson, Richard C. Litvinov, Dmitri |
author_facet | Khodadadi, Mohammad Chang, Long Trabuco, João R. C. Vu, Binh V. Kourentzi, Katerina Willson, Richard C. Litvinov, Dmitri |
author_sort | Khodadadi, Mohammad |
collection | PubMed |
description | This work presents a proof-of-concept demonstration of a novel inductive transducer, the femtoMag, that can be integrated with a lateral-flow assay (LFA) to provide detection and quantification of molecular biomarkers. The femtoMag transducer is manufactured using a low-cost printed circuit board (PCB) technology and can be controlled by relatively inexpensive electronics. It allows rapid high-precision quantification of the number (or amount) of superparamagnetic nanoparticle reporters along the length of an LFA test strip. It has a detection limit of 10(−10) emu, which is equivalent to detecting 4 ng of superparamagnetic iron oxide (Fe(3)O(4)) nanoparticles. The femtoMag was used to quantify the hCG pregnancy hormone by quantifying the number of 200 nm magnetic reporters (superparamagnetic Fe(3)O(4) nanoparticles embedded into a polymer matrix) immuno-captured within the test line of the LFA strip. A sensitivity of 100 pg/mL has been demonstrated. Upon further design and control electronics improvements, the sensitivity is projected to be better than 10 pg/mL. Analysis suggests that an average of 10(9) hCG molecules are needed to specifically bind 10(7) nanoparticles in the test line. The ratio of the number of hCG molecules in the sample to the number of reporters in the test line increases monotonically from 20 to 500 as the hCG concentration increases from 0.1 ng/mL to 10 ng/mL. The low-cost easy-to-use femtoMag platform offers high-sensitivity/high-precision target analyte quantification and promises to bring state-of-the-art medical diagnostic tests to the point of care. |
format | Online Article Text |
id | pubmed-6960494 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-69604942020-01-23 PCB-Based Magnetometer as a Platform for Quantification of Lateral-Flow Assays Khodadadi, Mohammad Chang, Long Trabuco, João R. C. Vu, Binh V. Kourentzi, Katerina Willson, Richard C. Litvinov, Dmitri Sensors (Basel) Article This work presents a proof-of-concept demonstration of a novel inductive transducer, the femtoMag, that can be integrated with a lateral-flow assay (LFA) to provide detection and quantification of molecular biomarkers. The femtoMag transducer is manufactured using a low-cost printed circuit board (PCB) technology and can be controlled by relatively inexpensive electronics. It allows rapid high-precision quantification of the number (or amount) of superparamagnetic nanoparticle reporters along the length of an LFA test strip. It has a detection limit of 10(−10) emu, which is equivalent to detecting 4 ng of superparamagnetic iron oxide (Fe(3)O(4)) nanoparticles. The femtoMag was used to quantify the hCG pregnancy hormone by quantifying the number of 200 nm magnetic reporters (superparamagnetic Fe(3)O(4) nanoparticles embedded into a polymer matrix) immuno-captured within the test line of the LFA strip. A sensitivity of 100 pg/mL has been demonstrated. Upon further design and control electronics improvements, the sensitivity is projected to be better than 10 pg/mL. Analysis suggests that an average of 10(9) hCG molecules are needed to specifically bind 10(7) nanoparticles in the test line. The ratio of the number of hCG molecules in the sample to the number of reporters in the test line increases monotonically from 20 to 500 as the hCG concentration increases from 0.1 ng/mL to 10 ng/mL. The low-cost easy-to-use femtoMag platform offers high-sensitivity/high-precision target analyte quantification and promises to bring state-of-the-art medical diagnostic tests to the point of care. MDPI 2019-12-10 /pmc/articles/PMC6960494/ /pubmed/31835468 http://dx.doi.org/10.3390/s19245433 Text en © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Khodadadi, Mohammad Chang, Long Trabuco, João R. C. Vu, Binh V. Kourentzi, Katerina Willson, Richard C. Litvinov, Dmitri PCB-Based Magnetometer as a Platform for Quantification of Lateral-Flow Assays |
title | PCB-Based Magnetometer as a Platform for Quantification of Lateral-Flow Assays |
title_full | PCB-Based Magnetometer as a Platform for Quantification of Lateral-Flow Assays |
title_fullStr | PCB-Based Magnetometer as a Platform for Quantification of Lateral-Flow Assays |
title_full_unstemmed | PCB-Based Magnetometer as a Platform for Quantification of Lateral-Flow Assays |
title_short | PCB-Based Magnetometer as a Platform for Quantification of Lateral-Flow Assays |
title_sort | pcb-based magnetometer as a platform for quantification of lateral-flow assays |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6960494/ https://www.ncbi.nlm.nih.gov/pubmed/31835468 http://dx.doi.org/10.3390/s19245433 |
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