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Dual Crosslinked Collagen/Chitosan Film for Potential Biomedical Applications

The application of polymeric biomaterial scaffolds utilizing crosslinking strategy has become an effective approach in these days. In the present study, the development and characterization of collagen–chitosan hydrogel film has been reported on using dual crosslinking agent’s, i.e., tannic acid and...

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Autores principales: Shah, Rushita, Stodulka, Pavel, Skopalova, Katerina, Saha, Petr
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6960699/
https://www.ncbi.nlm.nih.gov/pubmed/31847318
http://dx.doi.org/10.3390/polym11122094
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author Shah, Rushita
Stodulka, Pavel
Skopalova, Katerina
Saha, Petr
author_facet Shah, Rushita
Stodulka, Pavel
Skopalova, Katerina
Saha, Petr
author_sort Shah, Rushita
collection PubMed
description The application of polymeric biomaterial scaffolds utilizing crosslinking strategy has become an effective approach in these days. In the present study, the development and characterization of collagen–chitosan hydrogel film has been reported on using dual crosslinking agent’s, i.e., tannic acid and genipin simultaneously. Incorporation of genipin imparts a greenish-blue color to the polymeric film. The effect of dual crosslinking and their successful interaction within the matrix was evaluated by infrared analysis spectroscopy. The porosity of the film was examined using scanning electron microscopy (SEM). Results of TGA determine the intermediate thermal degradation. Further, the crosslinking phenomenon has found primary impact on the strength of the films. Enzymatic degradation for the films was performed with lysozyme and lipase. The cell adhesion and proliferation was also accomplished using mouse embryonic cell lines wherein the cells cultured on the dual crosslinked film. The thriving utilization of such dual crosslinked polymeric film finds their applications in ophthalmology especially as an implant for temporary injured cornea and skin tissue regeneration.
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spelling pubmed-69606992020-01-23 Dual Crosslinked Collagen/Chitosan Film for Potential Biomedical Applications Shah, Rushita Stodulka, Pavel Skopalova, Katerina Saha, Petr Polymers (Basel) Article The application of polymeric biomaterial scaffolds utilizing crosslinking strategy has become an effective approach in these days. In the present study, the development and characterization of collagen–chitosan hydrogel film has been reported on using dual crosslinking agent’s, i.e., tannic acid and genipin simultaneously. Incorporation of genipin imparts a greenish-blue color to the polymeric film. The effect of dual crosslinking and their successful interaction within the matrix was evaluated by infrared analysis spectroscopy. The porosity of the film was examined using scanning electron microscopy (SEM). Results of TGA determine the intermediate thermal degradation. Further, the crosslinking phenomenon has found primary impact on the strength of the films. Enzymatic degradation for the films was performed with lysozyme and lipase. The cell adhesion and proliferation was also accomplished using mouse embryonic cell lines wherein the cells cultured on the dual crosslinked film. The thriving utilization of such dual crosslinked polymeric film finds their applications in ophthalmology especially as an implant for temporary injured cornea and skin tissue regeneration. MDPI 2019-12-14 /pmc/articles/PMC6960699/ /pubmed/31847318 http://dx.doi.org/10.3390/polym11122094 Text en © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Shah, Rushita
Stodulka, Pavel
Skopalova, Katerina
Saha, Petr
Dual Crosslinked Collagen/Chitosan Film for Potential Biomedical Applications
title Dual Crosslinked Collagen/Chitosan Film for Potential Biomedical Applications
title_full Dual Crosslinked Collagen/Chitosan Film for Potential Biomedical Applications
title_fullStr Dual Crosslinked Collagen/Chitosan Film for Potential Biomedical Applications
title_full_unstemmed Dual Crosslinked Collagen/Chitosan Film for Potential Biomedical Applications
title_short Dual Crosslinked Collagen/Chitosan Film for Potential Biomedical Applications
title_sort dual crosslinked collagen/chitosan film for potential biomedical applications
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6960699/
https://www.ncbi.nlm.nih.gov/pubmed/31847318
http://dx.doi.org/10.3390/polym11122094
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