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Identification and molecular characterization of Wolbachia strains in natural populations of Aedes albopictus in China

BACKGROUND: Aedes albopictus is naturally infected with Wolbachia spp., maternally transmitted bacteria that influence the reproduction of hosts. However, little is known regarding the prevalence of infection, multiple infection status, and the relationship between Wolbachia density and dengue outbr...

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Autores principales: Hu, Yaping, Xi, Zhiyong, Liu, Xiaobo, Wang, Jun, Guo, Yuhong, Ren, Dongsheng, Wu, Haixia, Wang, Xiaohua, Chen, Bin, Liu, Qiyong
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6961339/
https://www.ncbi.nlm.nih.gov/pubmed/31937373
http://dx.doi.org/10.1186/s13071-020-3899-4
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author Hu, Yaping
Xi, Zhiyong
Liu, Xiaobo
Wang, Jun
Guo, Yuhong
Ren, Dongsheng
Wu, Haixia
Wang, Xiaohua
Chen, Bin
Liu, Qiyong
author_facet Hu, Yaping
Xi, Zhiyong
Liu, Xiaobo
Wang, Jun
Guo, Yuhong
Ren, Dongsheng
Wu, Haixia
Wang, Xiaohua
Chen, Bin
Liu, Qiyong
author_sort Hu, Yaping
collection PubMed
description BACKGROUND: Aedes albopictus is naturally infected with Wolbachia spp., maternally transmitted bacteria that influence the reproduction of hosts. However, little is known regarding the prevalence of infection, multiple infection status, and the relationship between Wolbachia density and dengue outbreaks in different regions. Here, we assessed Wolbachia infection in natural populations of Ae. albopictus in China and compared Wolbachia density between regions with similar climates, without dengue and with either imported or local dengue. RESULTS: To explore the prevalence of Wolbachia infection, Wolbachia DNA was detected in mosquito samples via PCR amplification of the 16S rRNA gene and the surface protein gene wsp. We found that 93.36% of Ae. albopictus in China were positive for Wolbachia. After sequencing gatB, coxA, hcpA, ftsZ, fbpA and wsp genes of Wolbachia strains, we identified a new sequence type (ST) of wAlbB (464/465). Phylogenetic analysis indicated that wAlbA and wAlbB strains formed a cluster with strains from other mosquitoes in a wsp-based maximum likelihood (ML) tree. However, in a ML tree based on multilocus sequence typing (MLST), wAlbB STs (464/465) did not form a cluster with Wolbachia strains from other mosquitoes. To better understand the association between Wolbachia spp. and dengue infection, the prevalence of Wolbachia in Ae. albopictus from different regions (containing local dengue cases, imported dengue cases and no dengue cases) was determined. We found that the prevalence of Wolbachia was lower in regions with only imported dengue cases. CONCLUSIONS: The natural prevalence of Wolbachia infections in China was much lower than in other countries or regions. The phylogenetic relationships among Wolbachia spp. isolated from field-collected Ae. albopictus reflected the presence of dominant and stable strains. However, wAlbB (464/465) and Wolbachia strains did not form a clade with Wolbachia strains from other mosquitoes. Moreover, lower densities of Wolbachia in regions with only imported dengue cases suggest a relationship between fluctuations in Wolbachia density in field-collected Ae. albopictus and the potential for dengue invasion into these regions.
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spelling pubmed-69613392020-01-17 Identification and molecular characterization of Wolbachia strains in natural populations of Aedes albopictus in China Hu, Yaping Xi, Zhiyong Liu, Xiaobo Wang, Jun Guo, Yuhong Ren, Dongsheng Wu, Haixia Wang, Xiaohua Chen, Bin Liu, Qiyong Parasit Vectors Research BACKGROUND: Aedes albopictus is naturally infected with Wolbachia spp., maternally transmitted bacteria that influence the reproduction of hosts. However, little is known regarding the prevalence of infection, multiple infection status, and the relationship between Wolbachia density and dengue outbreaks in different regions. Here, we assessed Wolbachia infection in natural populations of Ae. albopictus in China and compared Wolbachia density between regions with similar climates, without dengue and with either imported or local dengue. RESULTS: To explore the prevalence of Wolbachia infection, Wolbachia DNA was detected in mosquito samples via PCR amplification of the 16S rRNA gene and the surface protein gene wsp. We found that 93.36% of Ae. albopictus in China were positive for Wolbachia. After sequencing gatB, coxA, hcpA, ftsZ, fbpA and wsp genes of Wolbachia strains, we identified a new sequence type (ST) of wAlbB (464/465). Phylogenetic analysis indicated that wAlbA and wAlbB strains formed a cluster with strains from other mosquitoes in a wsp-based maximum likelihood (ML) tree. However, in a ML tree based on multilocus sequence typing (MLST), wAlbB STs (464/465) did not form a cluster with Wolbachia strains from other mosquitoes. To better understand the association between Wolbachia spp. and dengue infection, the prevalence of Wolbachia in Ae. albopictus from different regions (containing local dengue cases, imported dengue cases and no dengue cases) was determined. We found that the prevalence of Wolbachia was lower in regions with only imported dengue cases. CONCLUSIONS: The natural prevalence of Wolbachia infections in China was much lower than in other countries or regions. The phylogenetic relationships among Wolbachia spp. isolated from field-collected Ae. albopictus reflected the presence of dominant and stable strains. However, wAlbB (464/465) and Wolbachia strains did not form a clade with Wolbachia strains from other mosquitoes. Moreover, lower densities of Wolbachia in regions with only imported dengue cases suggest a relationship between fluctuations in Wolbachia density in field-collected Ae. albopictus and the potential for dengue invasion into these regions. BioMed Central 2020-01-14 /pmc/articles/PMC6961339/ /pubmed/31937373 http://dx.doi.org/10.1186/s13071-020-3899-4 Text en © The Author(s) 2020 Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.
spellingShingle Research
Hu, Yaping
Xi, Zhiyong
Liu, Xiaobo
Wang, Jun
Guo, Yuhong
Ren, Dongsheng
Wu, Haixia
Wang, Xiaohua
Chen, Bin
Liu, Qiyong
Identification and molecular characterization of Wolbachia strains in natural populations of Aedes albopictus in China
title Identification and molecular characterization of Wolbachia strains in natural populations of Aedes albopictus in China
title_full Identification and molecular characterization of Wolbachia strains in natural populations of Aedes albopictus in China
title_fullStr Identification and molecular characterization of Wolbachia strains in natural populations of Aedes albopictus in China
title_full_unstemmed Identification and molecular characterization of Wolbachia strains in natural populations of Aedes albopictus in China
title_short Identification and molecular characterization of Wolbachia strains in natural populations of Aedes albopictus in China
title_sort identification and molecular characterization of wolbachia strains in natural populations of aedes albopictus in china
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6961339/
https://www.ncbi.nlm.nih.gov/pubmed/31937373
http://dx.doi.org/10.1186/s13071-020-3899-4
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